[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-446627-105":59,"doc-detail-446627-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","oxidoreductase-gene-fabg-contributes-to-fungal-development-cell-wall-integrity-and-virulence-in-aspergillus-fumigatus","Oxidoreductase gene fabG contributes to fungal development, cell wall integrity, and virulence in Aspergillus fumigatus","","Aspergillus fumigatus, a major cause of invasive aspergillosis, depends on oxidoreductases for stress adaptation, yet the oxidoreductase gene fabG in fungal physiology and virulence is unclear. This study tests fabG function by generating fabG knockout and complemented strains, assessing growth, virulence in Galleria mellonella, antifungal susceptibility, transcriptome changes, and cell wall composition. Biochemical assays measure SOD activity, ROS, stress tolerance, macrophage co-culture outcomes, and CWI gene expression under caspofungin/rapamycin, identifying fabG as a regulator of growth, redox balance, CWI activation, and virulence with antifungal potential.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/oxidoreductase-gene-fabg-contributes-to-fungal-development-cell-wall-integrity-and-virulence-in-aspergillus-fumigatus/446627/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/oxidoreductase-gene-fabg-contributes-to-fungal-development-cell-wall-integrity-and-virulence-in-aspergillus-fumigatus/446627.png","ImageObject",300,407,{"name":92,"@type":93},"Mabel","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-04","2026-09-29",true,{"@type":102,"interactionType":103,"userInteractionCount":81},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What role does fabG play in Aspergillus fumigatus?","Question",{"text":112,"@type":113},"The study shows that fabG is critical for growth, environmental adaptation, and virulence by modulating cell wall integrity (CWI) and redox homeostasis.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How were fabG knockout and complemented strains generated and evaluated?",{"text":117,"@type":113},"fabG knockout and complemented strains were constructed via homologous recombination, then tested using phenotypic growth assays, virulence in Galleria mellonella, and antifungal susceptibility, alongside transcriptomic and biochemical measurements.",{"name":119,"@type":110,"acceptedAnswer":120},"What effects did deleting fabG have on oxidative stress and cell wall integrity?",{"text":121,"@type":113},"ΔfabG displayed decreased SOD activity, elevated ROS, reduced stress tolerance, increased susceptibility to macrophage killing, and signs of CWI activation such as increased chitin, while CWI-related gene expression remained unchanged under caspofungin/rapamycin.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},446627,1790837832,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":81,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145},7971474920318,"https://ap-avatar.wpscdn.com/avatar/74000ee537e6b0ca360?x-image-process=image/resize,m_fixed,w_180,h_180&k=1788430171632181674","| Open Peer Review | Clinical Microbiology | Research Article  \nOxidoreductase gene fabG contributes to fungal development, cell wall integrity, and virulence in Aspergillus fumigatus  \nHeng Zhang,1 Hua Ni,2 Xinyi Tao,3 Tian Chen,3 Yi Zhang,4 Xiaolei Zhu,1 Yinping Chen,1 Mengqi Peng,1 Yi Sun1 AUTHOR AFFILIATIONS See affiliation list on p. 18.  \nABSTRACT Aspergillus fumigatus, a major cause of invasive aspergillosis, relies onoxidoreductases for stress adaptation. The role of the oxidoreductase gene fabGin fungal physiology and virulence remains unclear. This study aims to investigate the function of fabG in regulating A. fumigatus growth, virulence, redox homeostasis, and cell wall integrity (CWI) . fabG knockout (ΔfabG) and complemented strains were constructed via homologous recombination. Phenotypic assays evaluated hyphal growth, virulence in Galleria mellonella, and antifungal susceptibility. Transcriptomic profiling, cell wall composition analysis (chitin/β-glucan), biochemical assays (superoxide dismutase [SOD] activity, reactive oxygen species [ROS] levels, and stress tolerance), and macrophage co-culture experiments (phagocytosis/killing) were performed. CWI-related gene expression was assessed under caspofungin/rapamycin treatment. ΔfabG showed inhibited hyphal growth, reduced virulence, and decreased caspofungin sensitivity. Transcriptomics revealed altered oxidoreductase and mitogen-activated protein kinase signaling genes. Cell wall thickening (increased chitin) and enhanced tolerance to cell wall stressors indicated CWI activation. ΔfabG exhibited decreased SOD activity, elevated ROS, reduced stress tolerance, and increased susceptibility to macrophage killing. CWI-related gene expression was unchanged under caspofungin/rapamycin. fabG critically regulates A. fumigatus growth, environmental adaptation, and virulence by modulating CWI and redox homeostasis, highlighting its potential as an antifungal target.  \nIMPORTANCE Aspergillus fumigatus is a harmful fungus that can cause severe and often deadly infections in people with weakened immune systems. Understanding how this fungus adapts to stress and causes disease is crucial for developing new treatments. Our study reveals that the gene fabG plays a vital role in regulating the growth, stress response, and virulence of A. fumigatus. When FabG is missing, the fungus becomes less able to grow, resists environmental stresses poorly, and is less harmful to infected hosts. This gene controls the fungus’s ability to maintain internal balance during oxidative stress and strengthens its cell wall, a protective structure. Since FabG is critical for the fungus’s survival and virulence, it could be a promising target for new antifungal drugs. By blocking fabG, we might be able to develop more effective treatments against this dangerous pathogen.  \nKEYWORDS fabG, Aspergillus fumigatus, oxidoreductase, cell wall integrity, virulence  \nT he genus Aspergillus, comprising filamentous fungi ubiquitously distributed in soil,  \ndecaying organic matter, and air, includes Aspergillus fumigatus as one of the most virulent human pathogens (1, 2) . This species thrives due to its unique biological traits, such as thermotolerance, robust oxidative stress adaptation, and efficient spore dispersal (3–8) .  \nEditor Vanessa Ann Varaljay, The Ohio State University, Columbus, Ohio, USA  \nPeer Reviewer Hee-Soo Park, Kyungpook National University, Daegu, Republic of Korea  \nAddress correspondence to Yi Sun, [jzzxyysy@163.com](jzzxyysy@163.com).  \nHeng Zhang and Hua Ni contributed equally to this article. The author order was determined based on their contribution to the article.  \nauthors declare conflict of interest  \nThe rising prevalence of immunosuppressive therapies and organ transplantation has dramatically expanded the population at risk for invasive aspergillosis (IA) . Epidemiological data indicate that approximately 30 million individuals worldwide are threatened by IA, with over 1 mil","cbCaitfevKX2nI1E","https://ap.wps.com/l/cbCaitfevKX2nI1E","pdf",14088358,21,"English","# Abstract\n## Study aims and strategy\n## Experimental assays and analyses\n## Key findings and significance","[{\"question\":\"What role does fabG play in Aspergillus fumigatus?\",\"answer\":\"The study shows that fabG is critical for growth, environmental adaptation, and virulence by modulating cell wall integrity (CWI) and redox homeostasis.\"},{\"question\":\"How were fabG knockout and complemented strains generated and evaluated?\",\"answer\":\"fabG knockout and complemented strains were constructed via homologous recombination, then tested using phenotypic growth assays, virulence in Galleria mellonella, and antifungal susceptibility, alongside transcriptomic and biochemical measurements.\"},{\"question\":\"What effects did deleting fabG have on oxidative stress and cell wall integrity?\",\"answer\":\"ΔfabG displayed decreased SOD activity, elevated ROS, reduced stress tolerance, increased susceptibility to macrophage killing, and signs of CWI activation such as increased chitin, while CWI-related gene expression remained unchanged under caspofungin/rapamycin.\"}]","Oxidoreductase gene fabG contributes to fungal development, cell wall integrity, and virulence in Aspergillus fumigatus | PDF",1790716348,53]