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Although TERT promoter mutations are the most commonly altered site in HBV-related HCC, their prognostic value remains insufficiently defined. This study assessed the prognostic impact of TERT promoter mutations and the performance of optimized digital PCR. Among 66 post-hepatectomy patients, mutation status was linked to worse survival and higher recurrence risk.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/optimized-digital-polymerase-chain-reaction-enables-detection-of-telomerase-reverse-transcriptase-c228t-mutation-for-prognostic-assessment-in-hepatocellular-carcinoma/406605/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/optimized-digital-polymerase-chain-reaction-enables-detection-of-telomerase-reverse-transcriptase-c228t-mutation-for-prognostic-assessment-in-hepatocellular-carcinoma/406605.png","ImageObject",300,407,{"name":92,"@type":93},"McGucket","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-11-04","2026-09-28",true,{"@type":102,"interactionType":103,"userInteractionCount":52},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What clinical problem does the study address in HBV-related hepatocellular carcinoma?","Question",{"text":112,"@type":113},"It targets recurrence-driven poor prognosis and evaluates whether TERT promoter mutation status can improve prognostic assessment after hepatectomy in HBV-related HCC.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How was digital polymerase chain reaction (dPCR) optimized in this work?",{"text":117,"@type":113},"The dPCR assay was optimized by adding 7-deaza-dGTP, CviQ1, and EDTA to enhance detection sensitivity.",{"name":119,"@type":110,"acceptedAnswer":120},"What were the key findings comparing dPCR with Sanger sequencing?",{"text":121,"@type":113},"TERT promoter mutations were detected more often by dPCR than Sanger sequencing, with high concordance between methods. dPCR showed 100% sensitivity and 90% specificity, and TERT-mutant patients had reduced overall survival and higher recurrence risk.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},406605,1790699615,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":52,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":46,"language":139,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":140,"faqs":141,"seo_title":142,"seo_description":67,"update_tm":143,"read_time":31,"preview_page_count":4},1236954412713,"https://us-avatar.wpscdn.com/davatar_29158cc5080c5b710cf443261637dec0","| W | J | G | O | World Journal of\u003Cbr>Gastrointestinal Oncology |\n| --- | --- | --- | --- | --- |\n\nSubmit a Manuscript: [https://www.f6publishing.com](https://www.f6publishing.com) World J Gastrointest Oncol 2025 December 15; 17(12): 113289  \nDOI: 10.4251/wjgo.v17.i12.113289 ISSN 1948-5204 (online)  \nORIGINAL ARTICLE  \nBasic Study  \nOptimized digital polymerase chain reaction enables detection of telomerase reverse transcriptase C228T mutation for prognostic assessment in hepatocellular carcinoma  \nZulihumaer Aizimuaji, Nan Hu, Hai-Yang Li, Xi-Jun Wang, Sheng Ma, Ya-Ru Wang, Rui-Qi Zheng, Zhuo Li, Huan Zhao, Wei-Qi Rong, Ting Xiao  \nSpecialty type: Oncology  \nProvenance and peer review:  \nUnsolicited article; Externally peer reviewed.  \nPeer-review model: Single blind  \nPeer-review report’s classification Scientific Quality: Grade C  \nNovelty: Grade C  \nCreativity or Innovation: Grade C  \nScientific Significance: Grade B  \nP-Reviewer: Tan BB, PhD, Chief Physician, Professor, China  \nReceived: August 21, 2025  \nRevised: September 30, 2025  \nAccepted: October 29, 2025  \nPublished online: December 15, 2025  \nProcessing time: 112 Days and 18.3 Hours  \nZulihumaer Aizimuaji, Hai-Yang Li, Xi-Jun Wang, Sheng Ma, Ya-Ru Wang, Rui-Qi Zheng, Ting Xiao,  \nState Key Laboratory of Molecular Oncology, Department of Etiology and Carcinogenesis, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China  \nNan Hu, Wei-Qi Rong, Department of Hepatobiliary Surgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China  \nZhuo Li, Huan Zhao, Department of Pathology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and  \nPeking Union Medical College, Beijing 100021, China Co-first authors: Zulihumaer Aizimuaji and Nan Hu.  \nCo-corresponding authors: Wei-Qi Rong and Ting Xiao.  \nCorresponding author: Wei-Qi Rong, MD, Department of Hepatobiliary Surgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, No. 17 Panjiayuan South Lane, Beijing 100021, [China.](China. dr_rongweiqi@163.com)[ dr_rongweiqi@163.com](China. dr_rongweiqi@163.com)  \nAbstract  \nBACKGROUND  \nRecurrence remains the leading cause of poor prognosis in hepatocellular carcinoma (HCC), particularly among patients infected with hepatitis B virus (HBV) . The telomerase reverse transcriptase (TERT) promoter is the most frequently mutated site in HBV-related HCC; however, its prognostic significance isnot fully established.  \nAIM  \nTo evaluate the prognostic impact of TERT promoter mutations and efficiency of digital polymerase chain reaction (dPCR) .  \n WJGO  [https://www.wjgnet.com](https://www.wjgnet.com) 1 December 15, 2025  Volume 17  Issue 12   \nAizimuaji Z et al. TERT C228T mutations to prognose HBV-related HCC patients  \nMETHODS  \nA total of 66 HBV-related HCC patients who underwent hepatectomy were enrolled in this study. DNA extracted from fresh tumor tissues was analyzed for TERT promoter mutations using Sanger sequencing and dPCR. The dPCR assay was optimized by adding 7-deaza-dGTP, CviQ1, and ethylenediaminetetraacetic acid to improve detection sensitivity. Concordance between methods was assessed, and nomogram survival prediction models were developed to evaluate prognostic value based on mutation status.  \nRESULTS  \nTERT promoter mutations were detected in 26/66 (39.39%) cases by Sanger sequencing and 30/66 (45.45%) by dPCR. The two methods showed high concordance (93.939%, κ = 0.876), with dPCR demonstrating 100% sensitivity and 90% specificity. Patients harboring TERT promoter mutations exhibited reduced overall survival and higher recurrence risk. Nomogram models ","cbCaioBn4kN5033L","https://ap.wps.com/l/cbCaioBn4kN5033L","pdf",5701595,"English","# Abstract\n## Background\n## Aim\n## Methods\n## Results\n## Conclusion\n# Core Tip\n# Citation","[{\"question\":\"What clinical problem does the study address in HBV-related hepatocellular carcinoma?\",\"answer\":\"It targets recurrence-driven poor prognosis and evaluates whether TERT promoter mutation status can improve prognostic assessment after hepatectomy in HBV-related HCC.\"},{\"question\":\"How was digital polymerase chain reaction (dPCR) optimized in this work?\",\"answer\":\"The dPCR assay was optimized by adding 7-deaza-dGTP, CviQ1, and EDTA to enhance detection sensitivity.\"},{\"question\":\"What were the key findings comparing dPCR with Sanger sequencing?\",\"answer\":\"TERT promoter mutations were detected more often by dPCR than Sanger sequencing, with high concordance between methods. dPCR showed 100% sensitivity and 90% specificity, and TERT-mutant patients had reduced overall survival and higher recurrence risk.\"}]","Optimized digital polymerase chain reaction enables detection of telomerase reverse transcriptase C228T mutation for prognostic assessment in hepatocellular carcinoma | PDF",1790566693]