[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-459589-105":59,"doc-detail-459589-en":131},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":124,"head_meta":126,"extra_data":128,"updated_unix":130},105,"en","oip5-as1mir-223-3p-provides-potential-biomarkers-for-chronic-periodontitis-and-its-regulatory-role-in-inflammatory-responses","OIP5-AS1/miR-223-3p provides potential biomarkers for chronic periodontitis and its regulatory role in inflammatory responses","","Chronic periodontitis (CP) is a common chronic oral infectious disease. This study evaluates the clinical significance of the differentially expressed lncRNA OIP5-AS1 and miR-223-3p in CP patients and investigates the OIP5-AS1/miR-223-3p axis in an LPS-induced cell model. Serum OIP5-AS1 is downregulated while miR-223-3p is upregulated, and both show diagnostic potential by ROC analysis. Functional assays indicate OIP5-AS1 sponges miR-223-3p to inhibit LPS-driven inflammation, improve cell viability, and promote osteogenic differentiation.",{"@graph":69,"@context":123},[70,84,106],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/oip5-as1mir-223-3p-provides-potential-biomarkers-for-chronic-periodontitis-and-its-regulatory-role-in-inflammatory-responses/459589/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/oip5-as1mir-223-3p-provides-potential-biomarkers-for-chronic-periodontitis-and-its-regulatory-role-in-inflammatory-responses/459589.png","ImageObject",300,407,{"name":92,"@type":93},"WPS_1786070896","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-11-10","2026-09-30",true,{"@type":102,"interactionType":103,"userInteractionCount":105},"InteractionCounter",{"@type":104},"ViewAction",11,{"@type":107,"mainEntity":108},"FAQPage",[109,115,119],{"name":110,"@type":111,"acceptedAnswer":112},"What expression pattern of OIP5-AS1 and miR-223-3p is observed in CP patients?","Question",{"text":113,"@type":114},"OIP5-AS1 expression is lower in CP patients than in controls, while miR-223-3p expression is higher (both P \u003C 0.001).","Answer",{"name":116,"@type":111,"acceptedAnswer":117},"How were OIP5-AS1 and miR-223-3p evaluated for diagnostic value?",{"text":118,"@type":114},"Reverse transcription quantitative PCR measured expression levels, and receiver operating characteristic (ROC) analysis assessed the ability to diagnose CP versus healthy controls.",{"name":120,"@type":111,"acceptedAnswer":121},"What role does the OIP5-AS1/miR-223-3p axis play in LPS-induced cellular inflammation?",{"text":122,"@type":114},"Experiments indicate OIP5-AS1 sponges miR-223-3p, inhibiting LPS-induced inflammatory responses and improving impaired cell proliferation.","https://schema.org",{"og:url":83,"og:type":125,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":127,"canonical":83},"index,follow",{"doc_id":129,"site_id":62},459589,1790811731,{"code":4,"msg":5,"data":132},{"doc_id":129,"user_id":133,"nickname":92,"user_avatar":134,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":135,"file_id":136,"file_url":137,"file_type":138,"file_size":139,"view_count":105,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":105,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145,"preview_page_count":4},549768072016,"https://ap-avatar.wpscdn.com/davatar_155a257f0dc6eb9ab79c44ca47cae57d","[www. nature.com/scientificreports](www. nature.com/scientificreports)  \nOPEN  \nOIP5-AS1/miR-223-3p provides potential biomarkers for chronic periodontitis and its regulatory role in inflammatory responses  \nHuijie Yu1,3, Tianhua Li1,3, Jie Liu1, Fengchun Hu1, Ling Yu2 & Yonggang Wang1􀀍  \nChronic periodontitis (CP) is a common chronic oral infectious disease. This study evaluated the clinical significance of differentially expressed OIP5-AS1 and miR-223-3p in CP patients and investigated the biological function of the OIP5-AS1/miR-223-3p axis in a cell model induced by LPS. One hundred and eighteen CP patients were enrolled to provide clinical samples and data. OIP5-AS1 and miR-223-3p expression were detected by reverse transcription quantitative PCR, and their clinical application in diagnose CP was evaluated by receiver operative curve (ROC) analysis. Bioinformatics prediction and dual-luciferase reporter assay confirmed the interaction between OIP5-AS1 and miR-223-3p. Human periodontal ligament cells (HPLCs) were treated with LPS to establish disease cell model, and the effects of OIP5-AS1/miR-223-3p on cell viability and inflammation were examined using CCK-8 and  \nELISA. In CP patients, OIP5-AS1 expression was lower and miR-223-3p expression was higher than in the control group (both P \u003C 0.001). Serum OIP5-AS1 and miR-223-3p showed considerable ability to diagnose CP from healthy controls (AUC = 0.816, sensitivity = 70.34%, specificity = 81.97% for OIP5-AS1; AUC = 0.837, sensitivity = 80.51%, specificity = 72.13% for miR-223-3p) . Cell experiments showed OIP5-AS1 improved LPS-induced impaired cell proliferation by sponging miR-223-3p. Additionally, OIP5-AS1 targets miR-223-3p and inhibits the inflammatory response induced by LPS, while promoting osteogenic differentiation of HPDLCs. Serum upregulatedOIP5-AS1 and downregulated miR-223-3p serve as potential diagnostic biomarkers for CP, and OIP5-AS1 might be involved in CP progression by sponging miR-223-3p.  \nKeywords Chronic periodontitis, OIP5-AS1, miR-223-3p, Inflammatory factors, Transfection  \nChronic periodontitis (CP) is a common chronic oral infectious disease worldwide1. The long-term accumulation of dental plaque around the gums triggers CP by inducing persistent inflammation in the gingival tissues2. The release of inflammatory factors disrupts the absorption and balance of alveolar bone, resulting in bone loss3. In diagnosis and evaluation of periodontal diseases, especially CP, attachment loss (AL), periodontal probing depth (PD), and gingival sulcus bleeding index (SBI) are three core indicators4,5. They are interrelated and respectively reflect different pathological conditions of periodontal tissues. Research has found that as periodontitis progresses, it can also induce other diseases, such as rheumatoid arthritis, chronic kidney disease, and cardiovascular diseases6–8. Thus, exploring novel potential biomarkers to achieve early diagnosis of CP, disease monitoring, and early warning of systemic risk has become a current research hotspot.  \nLong non-coding RNAs (lncRNAs) and microRNAs (miRNAs) are two important types of non-coding RNAs, which both playing key role in the regulation of gene expression and the occurrence and development of diseases9, 10. Many lncRNAs have been demonstrated to play key roles in the pathogenesis of diseases by acting as miRNA sponges11. Studies have found that lncRNA OIP5-AS1 plays a role in periodontitis12, 13. According to the detection of periodontal tissue specimens, the study found that OIP5-AS1 levels were significantly downregulated in periodontitis tissues compared with healthy controls14. More importantly, in the inflammatory cell model of human periodontal ligament cells (HPDLCs), the expression of OIP5-AS1 was downregulated, and it was further confirmed to regulate inflammatory responses15. However, the understanding of OIP5-AS1 in the progression of periodontitis, especially the related mechanisms, remains limited a","cbCaidc0eo10fPIN","https://ap.wps.com/l/cbCaidc0eo10fPIN","pdf",3041853,"English","# Background\n## Clinical indicators and disease impact\n## Non-coding RNAs in periodontitis\n# Study Design and Methods\n## Patient cohort and sample analysis\n## Expression detection and diagnostic evaluation\n## Interaction validation and cell modeling\n# Key Findings\n## Differential expression in CP patients\n## ROC diagnostic performance\n## OIP5-AS1/miR-223-3p regulatory effects on inflammation and viability","[{\"question\":\"What expression pattern of OIP5-AS1 and miR-223-3p is observed in CP patients?\",\"answer\":\"OIP5-AS1 expression is lower in CP patients than in controls, while miR-223-3p expression is higher (both P \\u003c 0.001).\"},{\"question\":\"How were OIP5-AS1 and miR-223-3p evaluated for diagnostic value?\",\"answer\":\"Reverse transcription quantitative PCR measured expression levels, and receiver operating characteristic (ROC) analysis assessed the ability to diagnose CP versus healthy controls.\"},{\"question\":\"What role does the OIP5-AS1/miR-223-3p axis play in LPS-induced cellular inflammation?\",\"answer\":\"Experiments indicate OIP5-AS1 sponges miR-223-3p, inhibiting LPS-induced inflammatory responses and improving impaired cell proliferation.\"}]","OIP5-AS1/miR-223-3p provides potential biomarkers for chronic periodontitis and its regulatory role in inflammatory responses | PDF",1790756315,28]