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Experimental evidence used western blot and multiple colon cancer functional assays (CCK-8, clonogenicity, wound healing, and Transwell).",{"@graph":14,"@context":73},[15,34,56],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/research-report/","Research & Report",3,{"item":32,"name":10,"@type":21,"position":33},"https://docshare.wps.com/document/nxph4-can-be-used-as-a-biomarker-for-pan-cancer-promotes-colon-cancer-progression/330477/",4,{"url":32,"name":10,"@type":35,"image":36,"author":41,"headline":10,"publisher":44,"fileFormat":47,"inLanguage":8,"description":12,"dateModified":48,"datePublished":49,"encodingFormat":47,"isAccessibleForFree":50,"interactionStatistic":51},"DigitalDocument",{"url":37,"@type":38,"width":39,"height":40},"https://docshare.wps.com/thumbnails/nxph4-can-be-used-as-a-biomarker-for-pan-cancer-promotes-colon-cancer-progression/330477.png","ImageObject",300,407,{"name":42,"@type":43},"Emma Wilson","Person",{"url":19,"name":45,"@type":46},"DocShare","Organization","application/pdf","2026-10-30","2026-09-21",true,{"@type":52,"interactionType":53,"userInteractionCount":55},"InteractionCounter",{"@type":54},"ViewAction",5,{"@type":57,"mainEntity":58},"FAQPage",[59,65,69],{"name":60,"@type":61,"acceptedAnswer":62},"How was NXPH4 evaluated as a pan-cancer biomarker?","Question",{"text":63,"@type":64},"Pan-cancer transcriptome and clinical data were obtained from TCGA, followed by Kaplan-Meier survival analysis and univariate Cox regression. Differential expression was validated in colon cancer by western blot.","Answer",{"name":66,"@type":61,"acceptedAnswer":67},"What methods were used to study the tumor immune microenvironment?",{"text":68,"@type":64},"CIBERSORT and TIMER algorithms quantified immune cell infiltration, and GSEA was applied to investigate NXPH4-associated functional pathways.",{"name":70,"@type":61,"acceptedAnswer":71},"What functional effects did NXPH4 show in colon cancer cells?",{"text":72,"@type":64},"NXPH4 knockdown significantly suppressed proliferation, invasion, and migration in HT-29 and HCT116 cells, supported by CCK-8, plate clonogenicity, wound healing, and Transwell assays.","https://schema.org",{"og:url":32,"og:type":75,"og:title":10,"og:site_name":45,"og:description":12},"article",{"robots":77,"canonical":32},"index,follow",{"doc_id":79,"site_id":7},330477,1790145546,{"code":4,"msg":82,"data":83},"success",[84,88,92,96,100,105,110,114,119,122,126],{"id":22,"doc_module":4,"doc_module_name":25,"category_name":85,"show_sort_weight":86,"slug":87},"Story & Novel",90,"story-novel",{"id":26,"doc_module":4,"doc_module_name":25,"category_name":89,"show_sort_weight":90,"slug":91},"Literature",80,"literature",{"id":33,"doc_module":4,"doc_module_name":25,"category_name":93,"show_sort_weight":94,"slug":95},"Exam",70,"exam",{"id":55,"doc_module":4,"doc_module_name":25,"category_name":97,"show_sort_weight":98,"slug":99},"Comic",60,"comic",{"id":101,"doc_module":4,"doc_module_name":25,"category_name":102,"show_sort_weight":103,"slug":104},6,"Technology",50,"technology",{"id":106,"doc_module":4,"doc_module_name":25,"category_name":107,"show_sort_weight":108,"slug":109},7,"Healthcare",40,"healthcare",{"id":111,"doc_module":4,"doc_module_name":25,"category_name":29,"show_sort_weight":112,"slug":113},8,30,"research-report",{"id":115,"doc_module":4,"doc_module_name":25,"category_name":116,"show_sort_weight":117,"slug":118},9,"Religion & Spirituality",20,"religion-spirituality",{"id":117,"doc_module":4,"doc_module_name":25,"category_name":120,"show_sort_weight":117,"slug":121},"World Cup","world-cup",{"id":123,"doc_module":4,"doc_module_name":25,"category_name":124,"show_sort_weight":123,"slug":125},10,"Lifestyle","lifestyle",{"id":127,"doc_module":4,"doc_module_name":25,"category_name":128,"show_sort_weight":55,"slug":129},19,"General","general",{"code":4,"msg":82,"data":131},{"doc_id":79,"user_id":132,"nickname":42,"user_avatar":133,"doc_module":4,"category_id":111,"category_name":29,"doc_title":10,"doc_description":12,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":55,"is_deleted":4,"is_public":22,"is_downloadable":22,"audit_status":22,"page_count":139,"language":140,"language_code":8,"site_id":7,"html_lang":8,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":12,"update_tm":144,"read_time":145,"preview_page_count":4},3848291630094,"https://eur-avatar.wpscdn.com/davatar_085a072bc5b1113ac321206ff7593b45","[www.aging-us.com](www.aging-us.com) AGING 2024, Vol. 16, No. 7  \nResearch Paper  \nNXPH4 can be used as a biomarker for pan-cancer and promotes colon cancer progression  \nZhipeng Zhang1,*, Pengfei Wang2,*, Siwen Chen3, Dezhi Xiang1, Jinzhen Chen1, Wanchang Huang1, Xiao Liu1, Tongwen Yi1, Dawei Wang4 ,Yunfei Pu4, Longfu He1, Hao Zhang5  \n1 Department of Anorectal Surgery, The Fifth Affiliated Hospital of Zunyi Medical University, Zhuhai, China 2 Department of Gastrointestinal Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an, China 3The Sixth Affiliated Hospital of Harbin Medical University, Harbin, China  \n4 Key Laboratory of Hepatosplenic Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, China  \n5 Department of Physiology, Zhuhai Campus of Zunyi Medical University, Zhuhai, China  \n*Equal contribution  \nCorrespondence to: Longfu He, Hao Zhang; email: [helongfu@zmuzh.edu.cn](helongfu@zmuzh.edu.cn), [zhanghao@zmuzh.edu.cn](zhanghao@zmuzh.edu.cn)[ ](zhanghao@zmuzh.edu.cn)[Keywords:](Keywords: NXPH4)[ NXPH4](Keywords: NXPH4), colon cancer, pan-cancer, biomarker, bioinformatics  \nReceived: August 29, 2023 Accepted: January 17, 2024 Published: April 12, 2024  \nCopyright: © 2024 Zhang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.  \nABSTRACT  \nNXPH4 promotes cancer proliferation and invasion. However, its specific role and mechanism in cancer remain unclear. Transcriptome and clinical data for pan-cancer were derived from the TCGA database. K-M survival curve and univariate Cox were used for prognostic analysis. CIBERSORT and TIMER algorithms were employed to calculate immune cell infiltration. Gene set enrichment analysis (GSEA) was employed for investigating the function of NXPH4 . Western blot verified differential expression of NXPH4 in colon cancer. Functional assays (CCK-8, plate clonogenicity assay, wound healing assay, and Transwell assay) confirmed the impact of NXPH4 on proliferation, invasion, and migration of colon cancer cells. Dysregulation of NXPH4 in pan-cancer suggests its potential as a diagnostic and prognostic marker for certain cancers, including colon and liver cancer. High expression of NXPH4 in pan-cancer might be associated with the increase in copy number and hypomethylation. NXPH4 expression in pan-cancer is substantially linked to immune cell infiltration in the immune microenvironment. NXPH4 expression is associated with the susceptibility to immunotherapy and chemotherapy. Western blot further confirmed the higher expression of NXPH4 in colon cancer. Knockdown of NXPH4 significantly suppresses proliferation, invasion, and migration of colon cancer cell lines HT-29 and HCT116, as validated by functional assays.  \nINTRODUCTION  \nThe incidence of cancer has gradually increased since the start of the twenty-first century. As a result, it has grown to be a significant factor in both quality of life and life expectancy of people. It is primarily caused by genetic and environmental influences [1, 2] . Malignancies have a complex microenvironment of malignant cells, which makes it difficult to eradicate them [3] . Moreover, several reports have highlighted  \nthat the phenotype of malignancies exhibits a strong correlation with the tumor immune microenvironment [4] . The International Agency for Research on Cancer (IARC) of the World Health Organization reported over 19 million new cancer cases and nearly 10 million deaths from cancer in the world last year alone [1] . It is generally accepted that if a country has a relatively high life expectancy, a high level of education, and a high standard of living, it tends to have a higher incidence of cancer [5] . Surgery, radiotherapy, and chemotherapy  \n5866  \nare the traditional means of cancer treatment in clinical pr","cbCaifam2WlWf1fm","https://ap.wps.com/l/cbCaifam2WlWf1fm","pdf",4191971,21,"English","# Abstract\n# Introduction\n# Materials and Methods\n# Results\n## Prognostic and survival analyses\n## Immune infiltration and enrichment\n# Discussion","[{\"question\":\"How was NXPH4 evaluated as a pan-cancer biomarker?\",\"answer\":\"Pan-cancer transcriptome and clinical data were obtained from TCGA, followed by Kaplan-Meier survival analysis and univariate Cox regression. Differential expression was validated in colon cancer by western blot.\"},{\"question\":\"What methods were used to study the tumor immune microenvironment?\",\"answer\":\"CIBERSORT and TIMER algorithms quantified immune cell infiltration, and GSEA was applied to investigate NXPH4-associated functional pathways.\"},{\"question\":\"What functional effects did NXPH4 show in colon cancer cells?\",\"answer\":\"NXPH4 knockdown significantly suppressed proliferation, invasion, and migration in HT-29 and HCT116 cells, supported by CCK-8, plate clonogenicity, wound healing, and Transwell assays.\"}]","NXPH4 can be used as a biomarker for pan-cancer - promotes colon cancer progression | PDF",1789989104,53]