[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-618928-105":59,"doc-detail-618928-en":124},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":117,"head_meta":119,"extra_data":121,"updated_unix":123},105,"en","npc1-function-requirement-for-ebola-and-marburg-virus-infection","NPC1 Function Requirement for Ebola and Marburg Virus Infection","","This research document, published in Nature, identifies the crucial role of the Niemann-Pick C1 (NPC1) protein in the entry and infection process of Ebola (EboV) and Marburg (MarV) viruses. Through a series of experiments using U18666A inhibitors, shRNA knockdown techniques in human umbilical vein endothelial cells (HUVECs), and mouse challenge models, the study demonstrates that NPC1 is an essential host factor for these viruses. The findings reveal that without functional NPC1, the viruses fail to initiate effective infection, highlighting a significant vulnerability in the viral entry pathway. The paper includes detailed data on viral growth yields, infection rates, survival percentages in wild-type versus NPC1-knockout mice, and a mechanistic model illustrating the virus's dependence on the HOPS complex, cathepsin (CTSB), and NPC1 for successful cytoplasmic release. This work provides fundamental insights into the cellular biology of filovirus infections and suggests potential therapeutic targets for intervention.",{"@graph":69,"@context":116},[70,84,99],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/npc1-function-requirement-for-ebola-and-marburg-virus-infection/618928/",{"url":83,"name":65,"@type":85,"author":86,"headline":65,"publisher":89,"fileFormat":92,"inLanguage":63,"description":67,"dateModified":93,"datePublished":93,"encodingFormat":92,"isAccessibleForFree":94,"interactionStatistic":95},"DigitalDocument",{"name":87,"@type":88},"Rhys","Person",{"url":74,"name":90,"@type":91},"DocShare","Organization","application/octet-stream","2026-10-20",true,{"@type":96,"interactionType":97,"userInteractionCount":4},"InteractionCounter",{"@type":98},"ViewAction",{"@type":100,"mainEntity":101},"FAQPage",[102,108,112],{"name":103,"@type":104,"acceptedAnswer":105},"What is the role of the NPC1 protein in viral infection according to the study?","Question",{"text":106,"@type":107},"The study demonstrates that NPC1 is a critical host factor required for the successful infection and entry of both Ebola and Marburg viruses into cells.","Answer",{"name":109,"@type":104,"acceptedAnswer":110},"How did the researchers test the effect of NPC1 on viral growth?",{"text":111,"@type":107},"The researchers utilized U18666A drug treatment, shRNA-mediated knockdown of NPC1, and genetically modified knockout mice to compare viral growth and host survival against control groups.",{"name":113,"@type":104,"acceptedAnswer":114},"What are the primary factors identified for viral entry in the provided model?",{"text":115,"@type":107},"The model identifies CTSB, the HOPS complex (including various VPS subunits), and NPC1 as the critical factors facilitating the cytoplasmic release of the virus.","https://schema.org",{"og:url":83,"og:type":118,"og:title":65,"og:site_name":90,"og:description":67},"article",{"robots":120,"canonical":83},"index,follow",{"doc_id":122,"site_id":62},618928,1792462014,{"code":4,"msg":5,"data":125},{"doc_id":122,"user_id":126,"nickname":87,"user_avatar":127,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":128,"file_id":129,"file_url":130,"file_type":131,"file_size":132,"view_count":4,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":8,"language":133,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":134,"faqs":135,"seo_title":136,"seo_description":67,"update_tm":123,"read_time":81},687207024643,"https://ap-avatar.wpscdn.com/davatar_3d24733baf745e90a7e4bdd5f77d97b2","JE Carette et al. Nature 000, 1-4 (2011) doi:10.1038/nature10348\nNPC1 function is required for infection by \u000bauthentic Ebola and Marburg viruses.","cbCaidVxtvvJIniQ","https://ap.wps.com/l/cbCaidVxtvvJIniQ","ppt",589824,"English","# Viral Infection and NPC1 Dependence\n## Experimental Evidence for NPC1 Requirement\n## Mechanistic Model of Viral Entry","[{\"question\":\"What is the role of the NPC1 protein in viral infection according to the study?\",\"answer\":\"The study demonstrates that NPC1 is a critical host factor required for the successful infection and entry of both Ebola and Marburg viruses into cells.\"},{\"question\":\"How did the researchers test the effect of NPC1 on viral growth?\",\"answer\":\"The researchers utilized U18666A drug treatment, shRNA-mediated knockdown of NPC1, and genetically modified knockout mice to compare viral growth and host survival against control groups.\"},{\"question\":\"What are the primary factors identified for viral entry in the provided model?\",\"answer\":\"The model identifies CTSB, the HOPS complex (including various VPS subunits), and NPC1 as the critical factors facilitating the cytoplasmic release of the virus.\"}]","NPC1 Function Requirement for Ebola and Marburg Virus Infection | PPT"]