[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-454048-105":59,"doc-detail-454048-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","novel-adenovirus-vaccine-vectors-lacking-thrombosis-associated-interactions-with-platelet-factor-4","Novel adenovirus vaccine vectors lacking thrombosis-associated interactions with platelet factor 4","","Adenovirus-based COVID-19 vaccines have been linked to rare thrombosis cases, associated with vector binding to platelet factor 4 (PF4). To reduce thrombosis risk, researchers screened 50 natural and hexon-modified adenoviruses, finding that Ad34, Ad80, and PF4-non-binding Ad5 variants lacking or shielding HVR1 do not bind PF4. PF4 interactions also altered Ad5 infectivity across diverse cell types. HVR1-deleted Ad5 and Ad34 vectors expressing SARS-CoV-2 spike S1 induced strong cellular immune responses in mice, supporting PF4 non-binding vectors as safer vaccine candidates.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/novel-adenovirus-vaccine-vectors-lacking-thrombosis-associated-interactions-with-platelet-factor-4/454048/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/novel-adenovirus-vaccine-vectors-lacking-thrombosis-associated-interactions-with-platelet-factor-4/454048.png","ImageObject",300,407,{"name":92,"@type":93},"Jake","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-11-04","2026-09-30",true,{"@type":102,"interactionType":103,"userInteractionCount":29},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What was the main goal of the study on adenovirus vaccine vectors?","Question",{"text":112,"@type":113},"To identify adenovirus vectors with lower thrombosis risk by reducing thrombosis-associated interactions with platelet factor 4 (PF4).","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"Which adenovirus vectors did not bind to PF4?",{"text":117,"@type":113},"Ad34, Ad80, and Ad5 vectors with deleted or chemically shielded hexon HVR1 did not bind to PF4.",{"name":119,"@type":110,"acceptedAnswer":120},"How did PF4 influence adenovirus infectivity and immune responses?",{"text":121,"@type":113},"PF4 interactions substantially modified Ad5 infectivity in multiple cell types, and PF4 non-binding vectors induced robust cellular immune responses in mice when expressing SARS-CoV-2 spike S1.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},454048,1790779192,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":29,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":46,"language":139,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":140,"faqs":141,"seo_title":142,"seo_description":67,"update_tm":143,"read_time":31,"preview_page_count":4},962084928904,"https://ap-avatar.wpscdn.com/davatar_155a257f0dc6eb9ab79c44ca47cae57d","iScience Article  \nNovel adenovirus vaccine vectors lacking thrombosis-associated interactions with platelet factor 4  \nGraphical abstract  \nHighlights  \n• We developed higher-throughput methods to study adenovirus binding to PF4  \n• Ad5-ΔHVR1, Ad34, and Ad80 do not bind to PF4  \n• Ad5-ΔHVR1 was non-inferior to Ad5 as a vaccine vector in mice  \n• PF4 modified the infectivity of adenoviruses that bind PF4  \nAuthors  \nErwan Sallard, Daniel Pembaur,  \nMatias Ciancaglini, ..., Daniel Pinschewer, Florian Kreppel, Anja Ehrhardt  \nCorrespondence  \n[florian.kreppel@uni-wh.de](florian.kreppel@uni-wh.de) (F.K.),  \n[anja.ehrhardt@uni-wh.de](anja.ehrhardt@uni-wh.de) (A.E.)  \nIn brief  \nMolecular biology; Immune response  \nSallard et al., 2026, iScience 29, 114329  \nJanuary 16, 2026 © 2025 Published by Elsevier Inc.  \n[https://doi.org/10.1016/j.isci.2025.114329](https://doi.org/10.1016/j.isci.2025.114329)  \nll  \niScience  \nll  \nOPEN ACCESS  \nArticle  \nNovel adenovirus vaccine vectors lacking thrombosis-associated interactions with platelet factor 4  \nErwan Sallard,1 Daniel Pembaur,2 Matias Ciancaglini,3 Lucie Manov-Bouard,4 Denice Weklak,2 Firas Hamdan,5,6,7,8 Chun Kit Chan,9,10 Franziska J¨onsson,2 Elise Chabot,1,11 Carmen Musielak,2 Elena Scurti,12 Sara Feola,5,6,7,8 Sebastian Schellhorn,1 Nissai Beaude,1,13 Katrin Schr¨oer,1 Daipayan Sarkar,9,14 Georgia Koukou,2 Xiaoyan Wang,15 Natascha Schmidt,2 Wibke Bayer,15 Malik Aydin,1,16 Vera Kemp,17 Alan L. Parker,18 Dirk Grimm,19 Tapani Viitala,12,20 Vincenzo Cerullo,5,6,7,8,21 Abhishek Singharoy,9 Alexander T. Baker,4,9,18 Wenli Zhang,1 Daniel Pinschewer,3  \nFlorian Kreppel,2,22,* and Anja Ehrhardt1,22,23,*  \n1Virology and Microbiology, Center for Biomedical Education and Research (ZBAF), Department of Human Medicine, Faculty of Health, Witten/Herdecke University, 58453 Witten, Germany  \n2Chair of Biochemistry and Molecular Medicine, Center for Biomedical Education and Research (ZBAF), Department of Human Medicine, Faculty of Health, Witten/Herdecke University, 58453 Witten, Germany  \n3Division of Experimental Virology, Department of Biomedicine, University of Basel, 4051 Basel, Switzerland  \n4Accession Therapeutics Limited, OX4 2JZ Oxford, UK  \n5University of Helsinki, Faculty of Pharmacy, Laboratory of Immunovirotherapy, Drug Research Program Helsinki, Uusimaa, Helsinki, Finland  \n6Helsinki Institute of Life Science (HiLIFE), Fabianinkatu 33, University of Helsinki, 00710 Helsinki, Finland  \n7Translational Immunology Program (TRIMM), Faculty of Medicine Helsinki University, Helsinki, Finland  \n8Digital Precision Cancer Medicine Flagship (iCAN), University of Helsinki, Helsinki, Finland  \n9Biodesign Institute Center for Applied Structural Discovery & Biosense Network, School of Molecular Science, Arizona State University, Tempe, AZ, USA  \n10NIH P41 Biotechnology Resource Center for Macromolecular Modeling and Bioinformatics, Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana Champaign, Champaign, IL, USA  \n11´Ecole Normale Sup´erieure de Lyon, Lyon 1 Claude Bernard University, Lyon, France  \n12University of Helsinki, Faculty of Pharmacy, Drug Research Program, Division of Pharmaceutical Chemistry and Technology, 00014 Helsinki, Finland  \n13AgroParisTech, Paris-Saclay University, Saclay, France  \n14MSU-DOE Plant Research Laboratory, East Lansing, MI, USA  \n15Institute for Virology, University Hospital Essen, University Duisburg-Essen, Essen, Germany  \n16Laboratory of Experimental Pediatric Pneumology and Allergology, Center for Biomedical Education and Research (ZBAF), Department of Human Medicine, Faculty of Health, Witten/Herdecke University, 58453 Witten, Germany  \n17Department of Cell & Chemical Biology, Leiden University Medical Center, Leiden, the Netherlands  \n18Division of Cancer and Genetics, Cardiff University School of Medicine, Heath Park, CF14 4XN Cardiff, UK  \n19Department of Infectious Diseases/Virology, Section Viral Vector Technologies, Med","cbCait77CEqQKwgV","https://ap.wps.com/l/cbCait77CEqQKwgV","pdf",5821979,"English","# Highlights\n## PF4 binding screening and vector selection\n## Vaccine-vector performance in mice\n## PF4 effects on infectivity and tropism","[{\"question\":\"What was the main goal of the study on adenovirus vaccine vectors?\",\"answer\":\"To identify adenovirus vectors with lower thrombosis risk by reducing thrombosis-associated interactions with platelet factor 4 (PF4).\"},{\"question\":\"Which adenovirus vectors did not bind to PF4?\",\"answer\":\"Ad34, Ad80, and Ad5 vectors with deleted or chemically shielded hexon HVR1 did not bind to PF4.\"},{\"question\":\"How did PF4 influence adenovirus infectivity and immune responses?\",\"answer\":\"PF4 interactions substantially modified Ad5 infectivity in multiple cell types, and PF4 non-binding vectors induced robust cellular immune responses in mice when expressing SARS-CoV-2 spike S1.\"}]","Novel adenovirus vaccine vectors lacking thrombosis-associated interactions with platelet factor 4 | PDF",1790740078]