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This study investigates metabolic and epigenetic regulation mediated by nicotinamide N-methyltransferase (NNMT) in LUAD. Untargeted metabolomic analyses, single-cell RNA sequencing and multiplex immunofluorescence are used to identify disrupted metabolic states. Results indicate NNMT links nicotinamide metabolism to metastatic behavior and is enriched in cancer-associated fibroblasts.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/nicotinamide-n-methyltransferase-negatively-regulates-metastasis-promoting-property-of-cancer-associated-fibroblasts-in-lung-adenocarcinoma/331759/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/nicotinamide-n-methyltransferase-negatively-regulates-metastasis-promoting-property-of-cancer-associated-fibroblasts-in-lung-adenocarcinoma/331759.png","ImageObject",300,407,{"name":92,"@type":93},"Valentina","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-31","2026-09-21",true,{"@type":102,"interactionType":103,"userInteractionCount":56},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What problem does the study address in lung adenocarcinoma?","Question",{"text":112,"@type":113},"It focuses on recurrence and metastasis after radical resection and the unclear mechanisms driving them in LUAD.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How is NNMT investigated in this research?",{"text":117,"@type":113},"NNMT-related metabolic and epigenetic regulation is assessed using untargeted metabolomics, single-cell RNA sequencing, multiplex immunofluorescence, and RNA-seq plus chromatin immunoprecipitation-PCR.",{"name":119,"@type":110,"acceptedAnswer":120},"What are the key findings about NNMT and metastasis?",{"text":121,"@type":113},"NNMT expression shows an inverse association with LUAD metastasis, and NNMT is predominantly expressed in cancer-associated fibroblasts within tumor stromal regions.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},331759,1790097300,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":56,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145,"preview_page_count":4},13056703020460,"https://ap-avatar.wpscdn.com/avatar/be000253dac470eee5d?_k=1778207105932848923","DOI: 10.1002/cac2.12633  \nORIGINAL ARTICLE  \nNicotinamide N-methyltransferase negatively regulates metastasis-promoting property of  \ncancer-associated fibroblasts in lung adenocarcinoma  \nPeiyu Wang1,2, 3,4   Guangxi Wang5  Haoran Li1,2, 3  Yuyao Yuan5  Haiming Chen1,2, 3  Shaodong Wang1, 2, 3  Zewen Sun1,2, 3  Fanjie Meng6   \nYun Li1,2, 3  Fan Yang1,2, 3  Jun Wang1,2, 3  Kezhong Chen1,2, 3  Mantang Qiu1,2, 3   \n1 Department of Thoracic Surgery, Peking University People’s Hospital, Beijing, P. R. China  \n2Thoracic Oncology Institute, Peking University People’s Hospital, Beijing, P. R. China  \n3 Research Unit of Intelligence Diagnosis and Treatment in Early Non-small Cell Lung Cancer, Chinese Academy of Medical Sciences, 2021RU002, Peking University People’s Hospital, Beijing, P. R. China  \n4 Department of Thoracic Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, P. R. China  \n5 Department of Pathology, School of Basic Medical Sciences, Institute of Systems Biomedicine, Peking-Tsinghua Center for Life Sciences, Peking University Health Science Center, Beijing, P. R. China  \n6 Department of Thoracic Surgery, Beijing Institute of Respiratory Medicine and Beijing Chao Yang Hospital, Capital Medical University, Beijing, P. R. China  \nCorrespondence  \nMantang Qiu, Thoracic Oncology Institute/Department of Thoracic Surgery, Peking University People’s Hospital, No.11 Xizhimen South Street, Beijing 100044, P.  \nR. China.  \n[Email:](Email: qiumantang@163.com)[ qiumantang@163.com](Email: qiumantang@163.com)  \nKezhong Chen, Thoracic Oncology Institute/Department of Thoracic Surgery, Peking University People’s Hospital, No.11 Xizhimen South Street, Beijing 100044, P.  \nR. China.  \n[Email:](Email: mdkzchen@163.com)[ mdkzchen@163.com](Email: mdkzchen@163.com)  \nAbstract  \nBackground: Recurrence and metastasis remain significant challenges in lung adenocarcinoma (LUAD) after radical resection. The mechanisms behind the recurrence and metastasis of LUAD remain elusive, and deregulated cellular metabolism is suspected to play a significant role. This study explores the metabolic and epigenetic regulation mediated by nicotinamide N-methyl transferase (NNMT) in LUAD.  \nMethods: Untargeted metabolomic analyses were performed to detect metabolism irregularities. Single-cell RNA sequencing (RNA-seq) databasesand multiplex immunofluorescence analysis were used to identify the location  \nAbbreviations: AOX1, Aldehyde oxidase 1; CAF, Cancer-associated fibroblast; CCK-8, Cell Counting Kit-8; ChIP, Chromatin immunoprecipitation; COL3A1, Collagen type III alpha 1 chain; COL5A1, Collagen type V alpha 1 chain; DMEM, Dulbecco’s modified Eagle’s medium; ECM, Extracellular matrix; EGFR, Epidermal Growth Factor Receptor; FAP, Fibroblast activation protein; FBS, Fetal bovine serum; FFPE, Formalin-fixed  \nparaffin-embedded; GO, Gene ontology; GSEA, Gene Set Enrichment Analysis; H3K4me3, Histone H3 Lysine 4 Trimethylation; HE, Hematoxylin and Eosin; IHC, Immunohistochemistry; ITGA, Integrin alpha; KEGG, Kyoto Encyclopedia of Genes and Genome; LC-MS, Liquid chromatography-mass spectrometry; LNM, Lymph node metastasis; log2FC, log2FoldChange; LUAD, Lung adenocarcinoma; LUSC, Lung squamous cell carcinoma; MMP11, Matrix metalloproteinase 11; MNA, Methylnicotinamide; NAD+ , Nicotinamide adenine dinucleotide; NF, Normal fibroblast; NNMT, Nicotinamide N-methyl transferase; NSCLC, Non-small cell lung cancer; PBS, Phosphate Buffered Saline; QC, Quality control; RNA-seq, RNA sequencing;  \nRT-qPCR, Quantitative real-time PCR; SAH, S-adenosyl-L-homocysteine; SAM, S-adenosyl-methionine; SET1/MLL, Histone methyltransferases/mixed lineage leukemia; siRNA, Small interfering RNA; SMA, Smooth muscle actin (alpha); TP53, Tumor Protein 53; UHPLC, Ultra-High Performance Liquid Chromatography; WDR5, WD repeat domain 5 .  \nThis is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use","cbCaiq06JAaD0LGc","https://ap.wps.com/l/cbCaiq06JAaD0LGc","pdf",21340599,28,"English","# Abstract\n## Background\n## Methods\n## Results\n## Abbreviations","[{\"question\":\"What problem does the study address in lung adenocarcinoma?\",\"answer\":\"It focuses on recurrence and metastasis after radical resection and the unclear mechanisms driving them in LUAD.\"},{\"question\":\"How is NNMT investigated in this research?\",\"answer\":\"NNMT-related metabolic and epigenetic regulation is assessed using untargeted metabolomics, single-cell RNA sequencing, multiplex immunofluorescence, and RNA-seq plus chromatin immunoprecipitation-PCR.\"},{\"question\":\"What are the key findings about NNMT and metastasis?\",\"answer\":\"NNMT expression shows an inverse association with LUAD metastasis, and NNMT is predominantly expressed in cancer-associated fibroblasts within tumor stromal regions.\"}]","Nicotinamide N-methyltransferase negatively regulates metastasis-promoting property of cancer-associated fibroblasts in lung adenocarcinoma | PDF",1789995003,71]