[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-457550-105":59,"doc-detail-457550-en":131},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":124,"head_meta":126,"extra_data":128,"updated_unix":130},105,"en","neo1-modulates-the-a1-astrocyte-polarization-in-subarachnoid-hemorrhage-through-the-cpla2-mavs-signaling-pathway","NEO1 modulates the A1 astrocyte polarization in subarachnoid hemorrhage through the cPLA2-MAVS signaling pathway","","Neuroinflammation after subarachnoid hemorrhage (SAH) drives multiple pathological cascades and worsens outcomes. Astrocytes can polarize into pro-inflammatory A1 and anti-inflammatory A2 phenotypes, yet the post-SAH polarization dynamics remain incompletely defined. Single-cell RNA sequencing shows increased A1 astrocytes after SAH and implicates NEO1 in this process. Astrocyte-specific NEO1 conditional knockout suppresses A1 polarization, reduces inflammatory factor release, and transcriptome data indicate decreased cPLA2, MAVS, and NF-κB signaling. cPLA2 overexpression reverses NEO1-loss effects in vitro, and miglustat administration mitigates neuroinflammation and improves functional recovery.",{"@graph":69,"@context":123},[70,84,106],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/neo1-modulates-the-a1-astrocyte-polarization-in-subarachnoid-hemorrhage-through-the-cpla2-mavs-signaling-pathway/457550/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/neo1-modulates-the-a1-astrocyte-polarization-in-subarachnoid-hemorrhage-through-the-cpla2-mavs-signaling-pathway/457550.png","ImageObject",300,407,{"name":92,"@type":93},"Bulrr","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-11-05","2026-09-30",true,{"@type":102,"interactionType":103,"userInteractionCount":105},"InteractionCounter",{"@type":104},"ViewAction",15,{"@type":107,"mainEntity":108},"FAQPage",[109,115,119],{"name":110,"@type":111,"acceptedAnswer":112},"What is the main role of NEO1 in astrocyte polarization after SAH?","Question",{"text":113,"@type":114},"NEO1 downregulation significantly inhibits A1 astrocyte polarization after SAH, reducing associated inflammatory factor release.","Answer",{"name":116,"@type":111,"acceptedAnswer":117},"How did the study assess astrocyte polarization and the underlying mechanism?",{"text":118,"@type":114},"Single-cell RNA sequencing identified increased A1 astrocytes after SAH, and transcriptome sequencing after astrocyte NEO1 knockout revealed reduced mRNA levels of cPLA2, MAVS, and NF-κB.",{"name":120,"@type":111,"acceptedAnswer":121},"What evidence supports the involvement of the cPLA2-MAVS signaling pathway?",{"text":122,"@type":114},"cPLA2-MAVS interaction is described as important for activating the NF-κB transcriptional program, and cPLA2 overexpression in primary astrocytes reverses the suppression of A1 polarization caused by NEO1 knockout.","https://schema.org",{"og:url":83,"og:type":125,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":127,"canonical":83},"index,follow",{"doc_id":129,"site_id":62},457550,1790822257,{"code":4,"msg":5,"data":132},{"doc_id":129,"user_id":133,"nickname":92,"user_avatar":134,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":135,"file_id":136,"file_url":137,"file_type":138,"file_size":139,"view_count":105,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":140,"language":141,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":142,"faqs":143,"seo_title":144,"seo_description":67,"update_tm":145,"read_time":146,"preview_page_count":4},5909892115043,"https://ap-avatar.wpscdn.com/davatar_6f874abed73319feea01a86fa6f0fab8","Wu et al. Journal of Neuroinflammation (2026) 23:6 Journal of Neuroinflammation  \n[https://doi.org/10.1186/s12974-025-03643-9](https://doi.org/10.1186/s12974-025-03643-9)  \nRESEARCH Open Access  \nNEO1 modulates the A1 astrocyte polarization in subarachnoid hemorrhage through the cPLA2-MAVS signaling pathway  \nYu Wu 1†, Boyang Wei 1†, Lei Jin 1†, Longxiang Li 1, Zeyu Yang 1, Can Li 1, Jiaming Zhou 1, Wenzhi Gong 1, Xin Feng 1, Shenquan Guo 1, Wenping Cheng 1, Ran Li 1, Shuyin Liang 1, Shixing Su 1, Fa Jin 1, Xin Zhang 1, Yanchao Liu 1, Chuanzhi Duan 1*, Xifeng Li 1* and Wenchao Liu 1*  \nAbstract  \nNeuroinflammation following subarachnoid hemorrhage (SAH) plays a critical role in multiple pathological pathways and poor prognosis. Several studies have indicated that resting astrocytes can polarize into at least two distinct phenotypes following SAH, pro-inflammatory A1 phenotype and anti-inflammatory A2 phenotype. However, the specific polarization patterns of astrocytes after SAH have not been fully elucidated. Targeting A1 astrocytes after SAH may represents a promising strategy for mitigating neuroinflammatory and enhancing neurological recovery. Our single-cell RNA sequencing revealed an increase in A1 astrocytes after SAH. Furthermore, the data suggested that NEO1 may be involved in astrocyte polarization. Then, we generated astrocyte-specific NEO1 conditional knockout (cKO) mice by crossing NEO1fl/fl with GFAP-Cre mice. Using an intravascular punctureinduced SAH mouse model, we demonstrated that NEO1 downregulation significantly inhibits A1 astrocyte polarization and reduces associated inflammatory factor release. To investigate the mechanisms underlying NEO1-mediated astrocyte polarization, we performed transcriptome sequencing. This analysis revealed significantly decreased mRNA levels of cPLA2, MAVS, and NF-κB following NEO1 knockout in astrocyte. cPLA2-MAVS interaction plays an important role in activating the NF-κB transcriptional program. Based on these findings, we hypothesized that NEO1 functions through this signaling pathway. To validate our hypothesis, we overexpressed cPLA2 in primary astrocytes using lentiviral vectors and performed in vitro experiments. Results demonstrated that cPLA2 overexpression effectively reversed NEO1 knockout-induced suppression of A1 polarization. Furthermore, miglustat administration significantly attenuated neuroinflammation and improved neurological functional recovery following SAH. Collectively, our findings reveal that NEO1 inhibition alleviates A1 astrocyte polarization following SAH through the cPLA2-MAVS pathway.  \n†Yu Wu, Boyang Wei and Lei Jin contributed equally to this work.  \n*Correspondence: Chuanzhi Duan [doctor_duanzj@163.com](doctor_duanzj@163.com)[ ](doctor_duanzj@163.com)Xifeng Li  \n[nflxf@126.com](nflxf@126.com)[ ](nflxf@126.com)Wenchao Liu [liuwenchao_zj@126.com](liuwenchao_zj@126.com)  \nFull list of author information is available at the end of the article  \n© The Author(s) 2025. Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit [http://creati](http://creati","cbCaigE87VBgQVBK","https://ap.wps.com/l/cbCaigE87VBgQVBK","pdf",14568519,18,"English","# Abstract\n# Introduction","[{\"question\":\"What is the main role of NEO1 in astrocyte polarization after SAH?\",\"answer\":\"NEO1 downregulation significantly inhibits A1 astrocyte polarization after SAH, reducing associated inflammatory factor release.\"},{\"question\":\"How did the study assess astrocyte polarization and the underlying mechanism?\",\"answer\":\"Single-cell RNA sequencing identified increased A1 astrocytes after SAH, and transcriptome sequencing after astrocyte NEO1 knockout revealed reduced mRNA levels of cPLA2, MAVS, and NF-κB.\"},{\"question\":\"What evidence supports the involvement of the cPLA2-MAVS signaling pathway?\",\"answer\":\"cPLA2-MAVS interaction is described as important for activating the NF-κB transcriptional program, and cPLA2 overexpression in primary astrocytes reverses the suppression of A1 polarization caused by NEO1 knockout.\"}]","NEO1 modulates the A1 astrocyte polarization in subarachnoid hemorrhage through the cPLA2-MAVS signaling pathway | PDF",1790749702,45]