[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"doc-seo-376046-105":3,"doc-detail-376046-en":81,"detail-sidebar-cat-0-en-105":99},{"code":4,"msg":5,"data":6},0,"ok",{"site_id":7,"language":8,"slug":9,"title":10,"keywords":11,"description":12,"schema_data":13,"social_meta":74,"head_meta":76,"extra_data":78,"updated_unix":80},105,"en","nano-topography-enhanced-topological-cell-analysis-in-radiation-therapy-perspective","Nano-Topography Enhanced Topological-Cell-Analysis in Radiation-Therapy - Perspective","","Radiotherapy exposes cells to ionizing radiation and offers cancer control, yet radio-resistance can limit effectiveness. This perspective hypothesizes that nanoscale surface topography can alter the topology of cancer cell networks under radiation, enabling improved assessment of intrinsic or acquired radiation resistance. It proposes an experimental plan using cancer cells exposed to different RT forms, comparing 2D surface and 3D scaffold nanoscale architectures, and contrasting with traditional clonogenic assays.",{"@graph":14,"@context":73},[15,34,56],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/healthcare/","Healthcare",3,{"item":32,"name":10,"@type":21,"position":33},"https://docshare.wps.com/document/nano-topography-enhanced-topological-cell-analysis-in-radiation-therapy-perspective/376046/",4,{"url":32,"name":10,"@type":35,"image":36,"author":41,"headline":10,"publisher":44,"fileFormat":47,"inLanguage":8,"description":12,"dateModified":48,"datePublished":49,"encodingFormat":47,"isAccessibleForFree":50,"interactionStatistic":51},"DigitalDocument",{"url":37,"@type":38,"width":39,"height":40},"https://docshare.wps.com/thumbnails/nano-topography-enhanced-topological-cell-analysis-in-radiation-therapy-perspective/376046.png","ImageObject",300,407,{"name":42,"@type":43},"Aditya","Person",{"url":19,"name":45,"@type":46},"DocShare","Organization","application/pdf","2026-10-29","2026-09-24",true,{"@type":52,"interactionType":53,"userInteractionCount":55},"InteractionCounter",{"@type":54},"ViewAction",12,{"@type":57,"mainEntity":58},"FAQPage",[59,65,69],{"name":60,"@type":61,"acceptedAnswer":62},"What is the core hypothesis of this perspective on radiotherapy?","Question",{"text":63,"@type":64},"Nanoscale surface topography can influence the topology of cancer-cell networks under radiation, which may improve the assessment of radio-resistance in radiotherapy applications.","Answer",{"name":66,"@type":61,"acceptedAnswer":67},"How does the proposed approach differ from traditional methods like clonogenic assays?",{"text":68,"@type":64},"It analyzes how 2D surface and 3D scaffold nanoscale architectures affect cancer-cell network organization, offering a topology-centered viewpoint rather than relying primarily on clonogenic readouts.",{"name":70,"@type":61,"acceptedAnswer":71},"What elements does the perspective propose to study together?",{"text":72,"@type":64},"The combined effects of surface topography, scaffold geometry, and radiation treatment on the network topology, including how these responses are modulated by each cell population’s intrinsic radiation resistance.","https://schema.org",{"og:url":32,"og:type":75,"og:title":10,"og:site_name":45,"og:description":12},"article",{"robots":77,"canonical":32},"index,follow",{"doc_id":79,"site_id":7},376046,1790507635,{"code":4,"msg":82,"data":83},"success",{"doc_id":79,"user_id":84,"nickname":42,"user_avatar":85,"doc_module":4,"category_id":86,"category_name":29,"doc_title":10,"doc_description":12,"doc_content":87,"file_id":88,"file_url":89,"file_type":90,"file_size":91,"view_count":55,"is_deleted":4,"is_public":22,"is_downloadable":22,"audit_status":22,"page_count":92,"language":93,"language_code":8,"site_id":7,"html_lang":8,"table_of_contents":94,"faqs":95,"seo_title":96,"seo_description":12,"update_tm":97,"read_time":98},962085564549,"https://ap-avatar.wpscdn.com/davatar_085a072bc5b1113ac321206ff7593b45",7,"PERSPECTIVE  \n[www.advhealthmat.de](www.advhealthmat.de)  \nNano-Topography Enhanced Topological-Cell-Analysis in Radiation-Therapy  \nFrancesca Pagliari, Maria-Francesca Spadea, Pierre Montay-Gruel,  \nAnggraeini Puspitasari-Kokko, Joao Seco, Luca Tirinato, Angelo Accardo,* Francesco De Angelis,* and Francesco Gentile*  \nRadiotherapy (RT) is a cancer treatment technique that involves exposing cells to ionizing radiation, including X-rays, electrons, or protons. RT offers promise to treat cancer, however, some inherent limitations can hamper its performance. Radio-resistance, whether innate or acquired, refers to the ability of tumor cells to withstand treatment, making it a key factor in RT failure. This perspective hypothesizes that nanoscale surface topography can impact on the topology of cancer cells network under radiation, and that this understanding can possibly advance the assessment of cell radio-resistance in RT applications. An experimental plan is proposed to test this hypothesis, using cancer cells exposed to various RT forms. By examining the influence of 2D surface and 3D scaffold nanoscale architecture on cancer cells, this approach diverges from traditional methodologies, such as clonogenic assays, offering a novel viewpoint that integrates fields such as tissue engineering, artificial intelligence, and nanotechnology. The hypotheses at the base of this perspective not only may advance cancer treatment but also offers insights into the broader field of structural biology. Nanotechnology and label-free Raman phenotyping of biological samples are lenses through which scientists can possibly better elucidate the structure-function relationship in biological systems.  \n1. Introduction  \nThis perspective proposes to explore the combined effects of surface topography, scaffold geometry, and RT treatment on the topology of cancer cell networks, examining how these responses are modulated by the intrinsic radiation resistance (RR) of cells (Figure 1) . The work is structured as follows.  \nIn the introduction, we first briefly describe how RT works, and suggest that surface-enhanced topological cell analysis can be a powerful tool for RT assessment (1.1). Then, we discuss the notion that nanotopography can efficiently guide cell behavior, and the spatial arrangement of cells, i.e., structure (1.2) . Subsequently, we recall that, in biological systems, structure and function are correlated (1.3) providing examples of how, by measuring the internal architecture of a system one can have an indication of how that system works (1.4) . In the last section of the introduction,  \nF. Pagliari, J. Seco  \nDivision of BioMedical Physics in Radiation Oncology German Cancer Research Center  \n69120 Heidelberg, Germany M.-F. Spadea  \nInstitute of Biomedical Engineering Karlsruhe Institute of Technology (KIT)  \n76131 Karlsruhe, Germany  \nP. Montay-Gruel  \nRadiation Oncology Department Iridium Netwerk  \nAntwerp 2610, Belgium  \nThe ORCID identification number(s) for the author(s) of this article  \ncan be found under [https://doi.org/10.1002/adhm.202405187](https://doi.org/10.1002/adhm.202405187)[ ](https://doi.org/10.1002/adhm.202405187)© 2025 The Author(s). Advanced Healthcare Materials published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.  \nDOI: 10.1002/adhm.202405187  \nP. Montay-Gruel  \nAntwerp Research in Radiation Oncology (AreRO) Center for Oncological Research (CORE)  \nUniversity of Antwerp Antwerp 2020, Belgium  \nA. Puspitasari-Kokko  \nResearch and Development-HollandPTC Delft University of Technology  \nDelft, The Netherlands  \nJ. Seco  \nDepartment of Physics and Astronomy Heidelberg University  \nIm Neuenheimer Feld 227, 69120 Heidelberg, Germany L. Tirinato  \nDepartment of Medical and Surgical Sciences University Magna Graecia of Catanzaro Catanzaro 88100, Italy  \nA. Acca","cbCaiuUnEGVo3BYn","https://ap.wps.com/l/cbCaiuUnEGVo3BYn","pdf",9682396,15,"English","# 1. Introduction\n## RT mechanism and assessment with topological cell analysis\n## Nanotopography effects on cell behavior and structure\n## Structure-function correlation in biological systems\n# 2. Review and related methods\n## Conventional RT analysis and clonogenic assays\n## Cell-network topology techniques\n## Nanotopography as regulator of cell behavior\n## 3D scaffolds as in-vitro models for RT studies\n## Nanomedicine strategies to overcome radiation resistance\n# Proposed perspective approach\n## Experimental plan for nano-topography-enhanced topological cell analysis\n## Integration with tissue engineering, nanotechnology, and AI","[{\"question\":\"What is the core hypothesis of this perspective on radiotherapy?\",\"answer\":\"Nanoscale surface topography can influence the topology of cancer-cell networks under radiation, which may improve the assessment of radio-resistance in radiotherapy applications.\"},{\"question\":\"How does the proposed approach differ from traditional methods like clonogenic assays?\",\"answer\":\"It analyzes how 2D surface and 3D scaffold nanoscale architectures affect cancer-cell network organization, offering a topology-centered viewpoint rather than relying primarily on clonogenic readouts.\"},{\"question\":\"What elements does the perspective propose to study together?\",\"answer\":\"The combined effects of surface topography, scaffold geometry, and radiation treatment on the network topology, including how these responses are modulated by each cell population’s intrinsic radiation resistance.\"}]","Nano-Topography Enhanced Topological-Cell-Analysis in Radiation-Therapy - Perspective | PDF",1790217094,38,{"code":4,"msg":82,"data":100},[101,105,109,113,118,123,126,131,136,139,143],{"id":22,"doc_module":4,"doc_module_name":25,"category_name":102,"show_sort_weight":103,"slug":104},"Story & Novel",90,"story-novel",{"id":26,"doc_module":4,"doc_module_name":25,"category_name":106,"show_sort_weight":107,"slug":108},"Literature",80,"literature",{"id":33,"doc_module":4,"doc_module_name":25,"category_name":110,"show_sort_weight":111,"slug":112},"Exam",70,"exam",{"id":114,"doc_module":4,"doc_module_name":25,"category_name":115,"show_sort_weight":116,"slug":117},5,"Comic",60,"comic",{"id":119,"doc_module":4,"doc_module_name":25,"category_name":120,"show_sort_weight":121,"slug":122},6,"Technology",50,"technology",{"id":86,"doc_module":4,"doc_module_name":25,"category_name":29,"show_sort_weight":124,"slug":125},40,"healthcare",{"id":127,"doc_module":4,"doc_module_name":25,"category_name":128,"show_sort_weight":129,"slug":130},8,"Research & Report",30,"research-report",{"id":132,"doc_module":4,"doc_module_name":25,"category_name":133,"show_sort_weight":134,"slug":135},9,"Religion & Spirituality",20,"religion-spirituality",{"id":134,"doc_module":4,"doc_module_name":25,"category_name":137,"show_sort_weight":134,"slug":138},"World Cup","world-cup",{"id":140,"doc_module":4,"doc_module_name":25,"category_name":141,"show_sort_weight":140,"slug":142},10,"Lifestyle","lifestyle",{"id":144,"doc_module":4,"doc_module_name":25,"category_name":145,"show_sort_weight":114,"slug":146},19,"General","general"]