[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-454376-105":59,"doc-detail-454376-en":134},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":127,"head_meta":129,"extra_data":131,"updated_unix":133},105,"en","murine-implantation-chamber-formation-precedes-natural-and-artificial-decidualization","Murine implantation chamber formation precedes natural and artificial decidualization","","Pregnancy requires a mesenchymal-to-epithelial transition in uterine stroma termed decidualization. In humans, initiation begins without an embryo, motivating identification of embryo-independent molecular cues, while in mice it remains unclear whether implantation is necessary for embryo-free decidualization. Using estrogen-dependent implantation models, implantation chamber formation was shown to occur before decidualization. Focal stimuli—including embryos, Concanavalin A-coated beads, and oil droplets—generated V-shaped implantation chambers, sub-epithelial PTGS2 expression, and decidualization, whereas uncoated beads and un-fertilized eggs failed to do so.",{"@graph":69,"@context":126},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/murine-implantation-chamber-formation-precedes-natural-and-artificial-decidualization/454376/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/murine-implantation-chamber-formation-precedes-natural-and-artificial-decidualization/454376.png","ImageObject",300,407,{"name":92,"@type":93},"Aria Callaghan","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-11-06","2026-09-30",true,{"@type":102,"interactionType":103,"userInteractionCount":24},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118,122],{"name":109,"@type":110,"acceptedAnswer":111},"What cellular process is central to this study on pregnancy?","Question",{"text":112,"@type":113},"The study focuses on decidualization, a mesenchymal to epithelial transition of uterine stromal cells during pregnancy.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How did the researchers test whether implantation is needed for mouse decidualization?",{"text":117,"@type":113},"They used estrogen-dependent implantation models, including natural embryo-based implantation and artificial systems such as sesame oil and bead-based focal stimuli, to compare timing and outcomes.",{"name":119,"@type":110,"acceptedAnswer":120},"Which stimuli induced implantation chamber formation and decidualization?",{"text":121,"@type":113},"Embryos, Concanavalin A-coated beads, and oil droplets induced V-shaped implantation chambers, sub-epithelial PTGS2 expression, and decidualization.",{"name":123,"@type":110,"acceptedAnswer":124},"What evidence supports that collagen changes relate to implantation chamber formation?",{"text":125,"@type":113},"Second harmonic generation showed that during decidualization collagen fibers spread radially away from the implantation chamber regardless of which focal signal induced the chamber, indicating artificial decidualization initiates at that site.","https://schema.org",{"og:url":83,"og:type":128,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":130,"canonical":83},"index,follow",{"doc_id":132,"site_id":62},454376,1791301633,{"code":4,"msg":5,"data":135},{"doc_id":132,"user_id":136,"nickname":92,"user_avatar":137,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":138,"file_id":139,"file_url":140,"file_type":141,"file_size":142,"view_count":24,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":143,"language":144,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":145,"faqs":146,"seo_title":147,"seo_description":67,"update_tm":148,"read_time":149,"preview_page_count":4},962084926284,"https://ap-avatar.wpscdn.com/davatar_29158cc5080c5b710cf443261637dec0","bioRxiv preprint doi:: https://doi://doi.org/10.64898/2025.12.30.697086; this version posted December 31 , 2025. The copyright holder for this preprint (whichich was not certified by peer review) is the author/funder,, who has granted bioRxiv a license to display the preprint in perpetuity. It is made  \navailable under aCC-BY 4.0 International license.  \nMurine implantation chamber formation precedes natural and artificial decidualization  \nHarini Raghu Kumar1,2 , Noura Massri 1,2 , Aishwarya V Bhurke1,2 , Akanksha Kapur1,2 , Pooja Gadhiya1,2 , Ripla Arora1,2,*  \n1 Institute for Quantitative Health Science and Engineering, Michigan State University 2 Department of Obstetrics, Gynecology and Reproductive Biology, Michigan State University  \nShort title: Implantation chamber formation precedes decidualization  \nKeywords: Implantation chamber, Embryo, Decidualization, Pseudopregnant, Concanavalin A beads, Second Harmonic Generation  \n*Corresponding Author  \nRipla Arora  \nAssociate Professor  \nDepartment of Obstetrics, Gynecology and Reproductive Biology  \nInstitute for Quantitative Health Science and Engineering  \nMichigan State University  \n775 Woodlot Drive, Rm\\#3312  \nEast Lansing, MI-48824, USA  \n[ripla@msu.edu](ripla@msu.edu)  \nbioRxiv preprint doi: [https://doi.org/10.64898/2025.12.30.697086](https://doi.org/10.64898/2025.12.30.697086); this version posted December 31, 2025. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made  \navailable under aCC-BY 4.0 International license.  \nABSTRACT  \nDuring pregnancy uterine stromal cells undergo a mesenchymal to epithelial cell transition termed decidualization. In humans initiation of decidualization occurs in the absence of an embryo resulting in a need to identify embryo-independent molecular cues that can initiate decidualization. Although, similar to humans, decidualization in the mouse can be induced in the absence of an embryo, whether an implantation event is prerequisite for such decidualization isnot known. In this study using different models of estrogen-dependent implantation, including natural (embryo) and artificial (sesame oil, agarose only beads, and Concanavalin A coated agarose beads) we determined that implantation chamber formation precedes decidualization.  \nWe show that focal stimuli, including the embryo, Concanavalin A coated bead, and oil droplets, induce V-shaped implantation chambers that lead to sub-epithelial PTGS2 expression and decidualization. Unfertilized eggs and uncoated agarose blue beads fail to form an implantation chamber and do not initiate decidualization. Further, we show that lectins that share sugar binding properties with Concanavalin A can also induce a V-shaped implantation chamber.  \nFinally, using second harmonic generation we show that during decidualization collagen fibers spread radially away from the implantation chamber irrespective of the focal signal used for inducing the chamber. Thus, in the mouse artificial decidualization also initiates at the site of implantation chamber formation. These findings are critical when separating physical stimulusdependent, embryo-dependent and embryo-independent mechanisms of decidualization that underlie a successful pregnancy.  \nbioRxiv preprint doi: [https://doi.org/10.64898/2025.12.30.697086](https://doi.org/10.64898/2025.12.30.697086); this version posted December 31, 2025. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made  \navailable under aCC-BY 4.0 International license.  \n1 INTRODUCTION  \n2 Uterine architectural changes are key to embryo movement, embryo spacing, embryo-uterine  \n3 interactions and implantation chamber formation in early mouse pregnancy (Flores et al. , 2020;  \n4 Madhavan et al. , 2022) . Attachment of t","cbCaiolILKM5FpXv","https://ap.wps.com/l/cbCaiolILKM5FpXv","pdf",15812818,28,"English","# Abstract\n## Introduction","[{\"question\":\"What cellular process is central to this study on pregnancy?\",\"answer\":\"The study focuses on decidualization, a mesenchymal to epithelial transition of uterine stromal cells during pregnancy.\"},{\"question\":\"How did the researchers test whether implantation is needed for mouse decidualization?\",\"answer\":\"They used estrogen-dependent implantation models, including natural embryo-based implantation and artificial systems such as sesame oil and bead-based focal stimuli, to compare timing and outcomes.\"},{\"question\":\"Which stimuli induced implantation chamber formation and decidualization?\",\"answer\":\"Embryos, Concanavalin A-coated beads, and oil droplets induced V-shaped implantation chambers, sub-epithelial PTGS2 expression, and decidualization.\"},{\"question\":\"What evidence supports that collagen changes relate to implantation chamber formation?\",\"answer\":\"Second harmonic generation showed that during decidualization collagen fibers spread radially away from the implantation chamber regardless of which focal signal induced the chamber, indicating artificial decidualization initiates at that site.\"}]","Murine implantation chamber formation precedes natural and artificial decidualization | PDF",1790740590,71]