[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"doc-seo-373720-105":3,"doc-detail-373720-en":81,"detail-sidebar-cat-0-en-105":99},{"code":4,"msg":5,"data":6},0,"ok",{"site_id":7,"language":8,"slug":9,"title":10,"keywords":11,"description":12,"schema_data":13,"social_meta":74,"head_meta":76,"extra_data":78,"updated_unix":80},105,"en","multi-omics-dissection-of-fatty-acid-metabolism-heterogeneity-identifies-prdx1-as-a-prognostic-marker-in-bladder-cancer","Multi-omics dissection of fatty acid metabolism heterogeneity identifies PRDX1 as a prognostic marker in bladder cancer","","Fatty-acid metabolism (FAM) is rewired in bladder cancer, but how this reshapes intratumoral diversity and influences patient outcome remains unclear. The study integrates spatial and single-cell transcriptomic data to quantify FAM heterogeneity, using hdWGCNA and multiple enrichment scoring methods. Machine learning then pinpoints a high-predictive gene signature validated with functional assays and clinical specimens. PRDX1 emerges as a FAM-linked oncogenic biomarker associated with poorer prognosis.",{"@graph":14,"@context":73},[15,34,56],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/healthcare/","Healthcare",3,{"item":32,"name":10,"@type":21,"position":33},"https://docshare.wps.com/document/multi-omics-dissection-of-fatty-acid-metabolism-heterogeneity-identifies-prdx1-as-a-prognostic-marker-in-bladder-cancer/373720/",4,{"url":32,"name":10,"@type":35,"image":36,"author":41,"headline":10,"publisher":44,"fileFormat":47,"inLanguage":8,"description":12,"dateModified":48,"datePublished":49,"encodingFormat":47,"isAccessibleForFree":50,"interactionStatistic":51},"DigitalDocument",{"url":37,"@type":38,"width":39,"height":40},"https://docshare.wps.com/thumbnails/multi-omics-dissection-of-fatty-acid-metabolism-heterogeneity-identifies-prdx1-as-a-prognostic-marker-in-bladder-cancer/373720.png","ImageObject",300,407,{"name":42,"@type":43},"Franzy","Person",{"url":19,"name":45,"@type":46},"DocShare","Organization","application/pdf","2026-11-03","2026-09-23",true,{"@type":52,"interactionType":53,"userInteractionCount":55},"InteractionCounter",{"@type":54},"ViewAction",8,{"@type":57,"mainEntity":58},"FAQPage",[59,65,69],{"name":60,"@type":61,"acceptedAnswer":62},"What question does the study address about fatty-acid metabolism in bladder cancer?","Question",{"text":63,"@type":64},"It examines how fatty-acid metabolism heterogeneity relates to intratumoral diversity and patient outcome in bladder cancer.","Answer",{"name":66,"@type":61,"acceptedAnswer":67},"How did the researchers characterize FAM heterogeneity?",{"text":68,"@type":64},"They integrated spatial and single-cell transcriptomic approaches, applying hdWGCNA plus multiple enrichment/scoring methods to identify high-FAM modules.",{"name":70,"@type":61,"acceptedAnswer":71},"What is the significance of PRDX1 in this work?",{"text":72,"@type":64},"PRDX1 is upregulated in tumors, associates with poorer prognosis, is enriched in malignant epithelial cells, and its silencing reduces bladder cancer cell proliferation, migration, and invasion.","https://schema.org",{"og:url":32,"og:type":75,"og:title":10,"og:site_name":45,"og:description":12},"article",{"robots":77,"canonical":32},"index,follow",{"doc_id":79,"site_id":7},373720,1790624026,{"code":4,"msg":82,"data":83},"success",{"doc_id":79,"user_id":84,"nickname":42,"user_avatar":85,"doc_module":4,"category_id":86,"category_name":29,"doc_title":10,"doc_description":12,"doc_content":87,"file_id":88,"file_url":89,"file_type":90,"file_size":91,"view_count":55,"is_deleted":4,"is_public":22,"is_downloadable":22,"audit_status":22,"page_count":92,"language":93,"language_code":8,"site_id":7,"html_lang":8,"table_of_contents":94,"faqs":95,"seo_title":96,"seo_description":12,"update_tm":97,"read_time":98},2336478945519,"https://ap-avatar.wpscdn.com/davatar_085a072bc5b1113ac321206ff7593b45",7,"TYPE Original Research PUBLISHED 11 September 2025 DOI 10.3389/fimmu.2025.1669822  \nOPEN ACCESS  \nEDITED BY  \nGiandomenico Roviello, University of Firenze, Italy  \nREVIEWED BY  \nPietro Pepe, Cannizzaro Hospital, Italy Xinti Sun,  \nChinese Academy of Medical Sciences and Peking Union Medical College, China Chengjiang Liu,  \nAnhui Medical University, China  \n*CORRESPONDENCE  \nShan hui Liu  \n [liushh2014@lzu.edu.cn](liushh2014@lzu.edu.cn)[ ](liushh2014@lzu.edu.cn)Li Yang  \n [ery_yangli@lzu.edu.cn](ery_yangli@lzu.edu.cn)  \n†These authors have contributed equally to this work  \nRECEIVED 20 July 2025  \nACCEPTED 29 August 2025  \nPUBLISHED 11 September 2025  \nCITATION  \nWang L, Chang Z, Chen S-y, Yang J-w, Wang K-y, Li K-p, Wan S, Liu Sh and Yang L (2025) Multi-omics dissection of fatty acid metabolism heterogeneity identifies PRDX1 as a prognostic marker in bladder cancer. Front. Immunol. 16:1669822 .  \ndoi: 10.3389/fimmu.2025.1669822  \nCOPYRIGHT  \n© 2025 Wang, Chang, Chen, Yang, Wang, Li, Wan, Liu and Yang. This is an open-access article distributed under the terms of the  \nCreative Commons Attribution License (CC BY) .  \nThe use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.  \nMulti-omics dissection of fatty acid metabolism heterogeneity identifies PRDX1 as a prognostic marker in bladder cancer  \nLi Wang1,2†, Zhe Chang1,2†, Si-yu Chen 1,2†, Jian-wei Yang 1,2, Kang-yu Wang 1,2, Kun-peng Li 1,2, Shun Wan 1,2,  \nShan hui Liu 1,2* and Li Yang 1,2*  \n1 Department of Urology, Gansu Province Clinical Research Center for Urinary System Disease, The Second Hospital & Clinical Medical School, Lanzhou University, Lanzhou, China, 2 Institute of Urology, Gansu Province Clinical Research Center for Urinary System Disease, The Second Hospital & Clinical Medical School, Lanzhou University, Lanzhou, China  \nBackground: Fatty−acid metabolism (FAM) is rewired in bladder cancer (BLCA), yet its impact on intratumoral diversity and patient outcome is unclear. Methods: To characterize FAM heterogeneity, we integrated spatial and singlecell transcriptomic approaches . We employed high-dimensional weighted correlation network analysis (hdWGCNA) alongside five distinct enrichment methods (ssGSEA, AddModuleScore, AUCell, singscore, and UCell) to identify modules with elevated FAM activity. Subsequently, machine learning algorithms were applied to bulk RNA sequencing datasets to pinpoint the key gene with highest predictive value. This candidate underwent validation through functional experiments and analysis of clinical specimens.  \nResults: Malignant epithelial cells displayed the strongest FAM activity. Cross −platform scoring and co−expression analysis produced a refined high−FAM gene set. Integrating this signature with bulk datasets singled out PRDX1 as a key driver. PRDX1 was up−regulated in tumors, predicted poorer prognosis, and was enriched in malignant epithelial cells. Silencing PRDX1 curtailed BLCA cell proliferation, migration, and invasion.  \nConclusions: PRDX1 emerges as a FAM−linked oncogenic biomarker that fosters BLCA progression. These findings define the metabolic hierarchy of BLCA and nominate PRDX1 as a candidate target for personalized therapy.  \nKEYWORDS  \nfatty acid metabolism, BLCA, PRDX1, machine learning, urology  \n1 Introduction  \nBladder cancer (BLCA) represents a major urological malignancy, accounting for roughly 573,000 newly diagnosed patients and 212,000 fatalities worldwide during 2020 (1, 2) . Urothelial carcinoma comprises more than 90% of diagnoses in industrialized nations,  \nFrontiers in Immunology 01 [frontiersin.org](frontiersin.org)  \nwhere both geographic location and patient age significantly affect disease occurrence (3, 4) . Even with progre","cbCaihzn3SfA1djG","https://ap.wps.com/l/cbCaihzn3SfA1djG","pdf",9014688,17,"English","# Background\n# Methods\n# Results\n# Conclusions\n# Key evidence for molecular findings","[{\"question\":\"What question does the study address about fatty-acid metabolism in bladder cancer?\",\"answer\":\"It examines how fatty-acid metabolism heterogeneity relates to intratumoral diversity and patient outcome in bladder cancer.\"},{\"question\":\"How did the researchers characterize FAM heterogeneity?\",\"answer\":\"They integrated spatial and single-cell transcriptomic approaches, applying hdWGCNA plus multiple enrichment/scoring methods to identify high-FAM modules.\"},{\"question\":\"What is the significance of PRDX1 in this work?\",\"answer\":\"PRDX1 is upregulated in tumors, associates with poorer prognosis, is enriched in malignant epithelial cells, and its silencing reduces bladder cancer cell proliferation, migration, and invasion.\"}]","Multi-omics dissection of fatty acid metabolism heterogeneity identifies PRDX1 as a prognostic marker in bladder cancer | PDF",1790200303,43,{"code":4,"msg":82,"data":100},[101,105,109,113,118,123,126,130,135,138,142],{"id":22,"doc_module":4,"doc_module_name":25,"category_name":102,"show_sort_weight":103,"slug":104},"Story & Novel",90,"story-novel",{"id":26,"doc_module":4,"doc_module_name":25,"category_name":106,"show_sort_weight":107,"slug":108},"Literature",80,"literature",{"id":33,"doc_module":4,"doc_module_name":25,"category_name":110,"show_sort_weight":111,"slug":112},"Exam",70,"exam",{"id":114,"doc_module":4,"doc_module_name":25,"category_name":115,"show_sort_weight":116,"slug":117},5,"Comic",60,"comic",{"id":119,"doc_module":4,"doc_module_name":25,"category_name":120,"show_sort_weight":121,"slug":122},6,"Technology",50,"technology",{"id":86,"doc_module":4,"doc_module_name":25,"category_name":29,"show_sort_weight":124,"slug":125},40,"healthcare",{"id":55,"doc_module":4,"doc_module_name":25,"category_name":127,"show_sort_weight":128,"slug":129},"Research & Report",30,"research-report",{"id":131,"doc_module":4,"doc_module_name":25,"category_name":132,"show_sort_weight":133,"slug":134},9,"Religion & Spirituality",20,"religion-spirituality",{"id":133,"doc_module":4,"doc_module_name":25,"category_name":136,"show_sort_weight":133,"slug":137},"World Cup","world-cup",{"id":139,"doc_module":4,"doc_module_name":25,"category_name":140,"show_sort_weight":139,"slug":141},10,"Lifestyle","lifestyle",{"id":143,"doc_module":4,"doc_module_name":25,"category_name":144,"show_sort_weight":114,"slug":145},19,"General","general"]