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MRPL48, a mitochondrial ribosomal protein involved in energy production, has been implicated in other cancers, but its role in HCC is unknown. Methods: Bioinformatics analyses used TCGA, GEO, HCCDB, CPTAC, SMART, UALCAN, Kaplan–Meier plotter, cBioPortal, and MethSurv, followed by in vitro validation with quantitative RT-PCR, immunoblotting, and functional assays. Results: MRPL48 is overexpressed in HCC and shows significant prognostic value, supported by ROC and nomogram analyses, methylation associations, pathway correlations, immune infiltration links, and reduced proliferation, migration, and invasion after knockdown.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/mrpl48-is-a-novel-prognostic-and-predictive-biomarker-of-hepatocellular-carcinoma/381947/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/mrpl48-is-a-novel-prognostic-and-predictive-biomarker-of-hepatocellular-carcinoma/381947.png","ImageObject",300,407,{"name":92,"@type":93},"Guten tag","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-09-25","2026-09-24",true,{"@type":102,"interactionType":103,"userInteractionCount":14},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What is MRPL48, and why was it investigated in hepatocellular carcinoma (HCC)?","Question",{"text":112,"@type":113},"MRPL48 is a mitochondrial ribosomal protein involved in energy production. It has shown prognostic and functional relevance in other cancers, but its role in HCC remained unclear.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How was MRPL48 evaluated in the study?",{"text":117,"@type":113},"The study combined bioinformatics analyses across multiple public datasets with in vitro validation using quantitative RT-PCR, immunoblotting, and functional assays.",{"name":119,"@type":110,"acceptedAnswer":120},"What clinical and biological findings support MRPL48 as a biomarker for HCC?",{"text":121,"@type":113},"MRPL48 was highly overexpressed in HCC, with survival and regression analyses indicating significant prognostic value. ROC and nomogram analyses supported strong predictive ability, and methylation status, pathway correlations, immune infiltration associations, and knockdown effects reinforced its biological relevance.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},381947,1790308014,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":14,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145},687212321768,"https://ap-avatar.wpscdn.com/avatar/a0010bdbe886d2fe77?x-image-process=image/resize,m_fixed,w_180,h_180&k=1789897067658708522","Lin etal. European Journal  \nEuropean Journal of Medical Research (2023) 28:589  \n[https://doi.org/10.1186/s40001-023-01571-z](https://doi.org/10.1186/s40001-023-01571-z of Medical Research)[ of Medical Research](https://doi.org/10.1186/s40001-023-01571-z of Medical Research)  \n RESEARCH Open Access  \nMRPL48 is a novel prognostic and predictive biomarker of hepatocellular carcinoma  \nYu‑Xiang Lin2, Jun‑Yong Pan 1, Wen‑Du Feng 1, Tian‑Cong Huang 1 and Cheng‑Zong Li 1*  \nAbstract  \nBackground Hepatocellular carcinoma (HCC) is one of the most prevalent forms of cancer and poses a threat to the health and survival of humans. Mitochondrial ribosomal protein L48 (MRPL48) belongs to the mitochondrial ribosomal protein family, which participates in energy production. Studies have shown that MRPL48 can predict oste‑ osarcoma incidence and prognosis, as well as promotes colorectal cancer progression. However, the role of MRPL48 in HCC remains unknown.  \nMethods TCGA, GEO, HCCDB, CPTAC, SMART, UALCAN, Kaplan–Meier plotter, cBioPortal, and MethSurv were per‑ formed for bioinformatics purposes. Quantitative RT‑PCR, immunoblotting, and functional studies were conducted to validate the methodology in vitro.  \nResults MRPL48 was greatly overexpressed in HCC tissues, compared with healthy tissue, which was subsequently demonstrated in vitro as well. The survival and regression analyses showed that MRPL48 expression is of signifi‑ cant clinical prognostic value in HCC. The ROC curve and nomogram analysis indicated that MRPL48 is a power‑ ful predictor of HCC. MRPL48 methylation was adversely associated with the expression of MRPL48, and patients with a low level of methylation had poorer overall survival than those with a high level of methylation. GSEA showed that the expression of the MRPL48 was correlated with Resolution of Sister Chromatid Cohesion, Mitotic Prometaphase, Retinoblastoma Gene in Cancer, RHO Gtpases Activate Formins, Mitotic Metaphase and Anaphase, and Cell Cycle Checkpoints. An analysis of immune cell infiltration showed a significant association between MRPL48 and immune cell infiltration subsets, which impacted the survival of HCC patients. Additionally, MRPL48 knockdown reduced HCC cell proliferation, migration, and invasion in vitro.  \nConclusions We demonstrated that MRPL48 expression may be associated with HCC development and prognosis. These findings may open up new research directions and opportunities for the development of HCC treatments. Keywords Hepatocellular carcinoma, MRPL48, Prognostic value, Biomarker, Biological functions  \n*Correspondence: Cheng‑Zong Li[lichengzong@126.com](lichengzong@126.com)  \n1 Department of Hepatobiliary and Pancreatic Surgery, The Second Affiliated Hospital of Fujian Medical University (Donghai District), Quanzhou 36200, People’s Republic of China  \n2 Central Supply Service Department, The Second Affiliated Hospital of Fujian Medical University, Quanzhou 36200, People’s Republic of China  \nIntroduction  \nHepatocellular carcinoma (HCC) is the most common primary liver cancer worldwide and poses a serious public health concern. It is estimated that 905,677 new cases of hepatocellular carcinoma were diagnosed in 2020, and 830,180 died from the disease [1]. HCC has become the sixth most common malignancy and also the third leading cause of cancer-related deaths worldwide [2]. Thereis no doubt that surgical treatment of HCC is crucial for long-term survival. However, because HCC develops insidiously, over 80% of patients with HCC are first  \n© The Author(s) 2023. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in t","cbCaisV5BW4GhSkr","https://ap.wps.com/l/cbCaisV5BW4GhSkr","pdf",7738844,17,"English","# Abstract\n## Background\n## Methods\n## Results\n## Conclusions\n# Introduction","[{\"question\":\"What is MRPL48, and why was it investigated in hepatocellular carcinoma (HCC)?\",\"answer\":\"MRPL48 is a mitochondrial ribosomal protein involved in energy production. It has shown prognostic and functional relevance in other cancers, but its role in HCC remained unclear.\"},{\"question\":\"How was MRPL48 evaluated in the study?\",\"answer\":\"The study combined bioinformatics analyses across multiple public datasets with in vitro validation using quantitative RT-PCR, immunoblotting, and functional assays.\"},{\"question\":\"What clinical and biological findings support MRPL48 as a biomarker for HCC?\",\"answer\":\"MRPL48 was highly overexpressed in HCC, with survival and regression analyses indicating significant prognostic value. ROC and nomogram analyses supported strong predictive ability, and methylation status, pathway correlations, immune infiltration associations, and knockdown effects reinforced its biological relevance.\"}]","MRPL48 is a novel prognostic and predictive biomarker of hepatocellular carcinoma | PDF",1790247924,43]