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This work compares MPXV clade IIb replication efficiency with clades Ia and IIa by examining viral gene expression. OPG175 is highly expressed in IIb-infected cells, and manipulating OPG175 alters infectious titers. OPG175 overexpression activates Wnt signaling–related gene expression, and Wnt activation suppresses MPXV replication efficiency.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/mpox-virus-opg175-negatively-regulates-viral-replication-by-controlling-wnt-signaling/411483/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/mpox-virus-opg175-negatively-regulates-viral-replication-by-controlling-wnt-signaling/411483.png","ImageObject",300,407,{"name":92,"@type":93},"supergirl","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-11-05","2026-09-28",true,{"@type":102,"interactionType":103,"userInteractionCount":44},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What relationship does OPG175 have with MPXV replication efficiency?","Question",{"text":112,"@type":113},"OPG175 overexpression decreases MPXV infection efficiency, while OPG175 knockdown increases infection efficiency.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"Which MPXV clade shows higher OPG175 expression?",{"text":117,"@type":113},"OPG175 is highly expressed in MPXV clade IIb-infected cells.",{"name":119,"@type":110,"acceptedAnswer":120},"How does Wnt signaling influence MPXV replication in this study?",{"text":121,"@type":113},"OPG175 overexpression enhances Wnt signaling-related gene expression, and activation of Wnt signaling decreases MPXV replication efficiency.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},411483,1790766998,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":44,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145,"preview_page_count":4},962088121634,"https://ap-avatar.wpscdn.com/davatar_9964176cb1d06d4a9deccf72a44ae3dc","iScience Article  \nMpox virus OPG175 negatively regulates viral replication by controlling Wnt signaling  \nAuthors  \nYoshitaka Nakata, Masako Yamasaki, Yukio Watanabe,   , Kei Sato, Akatsuki Saito, Kazuo Takayama  \nCorrespondence  \n[keisato@g.ecc.u-tokyo.ac.jp](keisato@g.ecc.u-tokyo.ac.jp) (K.S.),  \n[sakatsuki@cc.miyazaki-u.ac.jp](sakatsuki@cc.miyazaki-u.ac.jp) (A.S.),  \n[takayama.kazuo@tmd.ac.jp](takayama.kazuo@tmd.ac.jp) (K.T.)  \nIn brief  \nImmunology; Virology; Transcriptomics  \n• OPG175 overexpression decreased MPXV infection efficiency  \n• OPG175 knockdown increased MPXV infection efficiency  \n• OPG175 overexpression activated Wnt signaling  \nNakata et al., 2025, iScience 28, 114105  \nDecember 19, 2025 © 2025 The Author(s) . Published by Elsevier Inc.  \n[https://doi.org/10.1016/j.isci.2025.114105](https://doi.org/10.1016/j.isci.2025.114105)  \nll  \niScience  \nll  \nOPEN ACCESS  \nArticle  \nMpox virus OPG175 negatively regulates viral replication by controlling Wnt signaling  \nYoshitaka Nakata,1,2 Masako Yamasaki,1 Yukio Watanabe,2 Keiya Uriu,3 Rina Hashimoto,1 Takuya Yamamoto,2,4,5 The Genotype to Phenotype Japan (G2P-Japan) Consortium, Kei Sato,3,6,7,8,9,10,11,* Akatsuki Saito,12,13,14,*  \nand Kazuo Takayama1,2,15,*  \n1Department of Synthetic Human Body System, Medical Research Laboratory, Institute of Integrated Research, Institute of Science Tokyo, Tokyo 113-8510, Japan  \n2Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto 606-8507, Japan  \n3Division of Systems Virology, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan  \n4Medical-risk Avoidance based on iPS Cells Team, RIKEN Center for Advanced Intelligence Project (AIP), Kyoto 606-8507, Japan  \n5Institute for the Advanced Study of Human Biology (WPI-ASHBi), Kyoto University, Kyoto 606-8303, Japan  \n6Graduate School of Medicine, The University of Tokyo, Tokyo 113-8654, Japan  \n7Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa 277-0882, Japan  \n8International Research Center for Infectious Diseases, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan  \n9International Vaccine Design Center, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan  \n10Collaboration Unit for Infection, Joint Research Center for Human Retrovirus infection, Kumamoto University, Kumamoto 860-0811, Japan  \n11Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand  \n12Department of Veterinary Science, Faculty of Agriculture, University of Miyazaki, Miyazaki 889-2192, Japan  \n13Graduate School of Medicine and Veterinary Medicine, University of Miyazaki, Miyazaki 889-1692, Japan  \n14Center for Animal Disease Control, University of Miyazaki, Miyazaki 889-2192, Japan  \n15Lead contact  \n*Correspondence: [keisato@g.ecc.u-tokyo.ac.jp](keisato@g.ecc.u-tokyo.ac.jp) (K.S.), [sakatsuki@cc.miyazaki-u.ac.jp](sakatsuki@cc.miyazaki-u.ac.jp) (A.S.), [takayama.kazuo@tmd.ac.jp](takayama.kazuo@tmd.ac.jp) (K.T.) [https://doi.org/10.1016/j.isci.2025.114105](https://doi.org/10.1016/j.isci.2025.114105)  \nSUMMARY  \nMpox virus (MPXV) infection exhibits different case fatality rates and symptoms depending on its clade. However, there has been insufficient molecular biological analysis of the differences between clades. Here, we investigated whether clades can be distinguished by focusing on the expression of MPXV genes. The replication efficiency of MPXV clade IIb, responsible for the 2022 mpox outbreak, was lower than that of clades Ia and IIa. We found that OPG175 was highly expressed in MPXV clade IIb-infected cells. Suppression of OPG175 expression significantly increased the infectious titer of MPXV, whereas OPG175 overexpression significantly decreased it. We also found that OPG175 overexpression enhanced the expression of Wnt signaling-related genes, and activation ofWnt signaling decreased MPXV replication efficiency. Therefore","cbCaiaFKg0M1YRcB","https://ap.wps.com/l/cbCaiaFKg0M1YRcB","pdf",3668124,15,"English","# Summary\n# Introduction\n## Mpox clades and outbreak context\n## MPXV genome structure and protein functions","[{\"question\":\"What relationship does OPG175 have with MPXV replication efficiency?\",\"answer\":\"OPG175 overexpression decreases MPXV infection efficiency, while OPG175 knockdown increases infection efficiency.\"},{\"question\":\"Which MPXV clade shows higher OPG175 expression?\",\"answer\":\"OPG175 is highly expressed in MPXV clade IIb-infected cells.\"},{\"question\":\"How does Wnt signaling influence MPXV replication in this study?\",\"answer\":\"OPG175 overexpression enhances Wnt signaling-related gene expression, and activation of Wnt signaling decreases MPXV replication efficiency.\"}]","Mpox virus OPG175 negatively regulates viral replication by controlling Wnt signaling | PDF",1790588683,38]