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This study evaluated lidocaine’s effects on oral epidermoid carcinoma OECM-1 and gingival epithelial SG cells using viability testing, flow cytometry, qPCR, western blotting, electric cell-substrate impedance sensing, and RNA sequencing. Lidocaine showed cell-type specific actions: suppression of OECM-1 proliferation and apoptosis with mitochondrial changes and autophagy, contrasted by distinct SG cell cycle and ROS responses, including synergism with cisplatin and 5-fluorouracil, suggesting therapeutic and wound-healing relevance beyond pain control.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/molecular-level-insights-into-the-therapeutic-potential-of-lidocaine-effects-on-proliferation-autophagy-and-cellular-impedance-in-human-oral-cells/352145/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/molecular-level-insights-into-the-therapeutic-potential-of-lidocaine-effects-on-proliferation-autophagy-and-cellular-impedance-in-human-oral-cells/352145.png","ImageObject",300,407,{"name":92,"@type":93},"anakgang17","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-25","2026-09-22",true,{"@type":102,"interactionType":103,"userInteractionCount":24},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What was the main goal of the study on lidocaine?","Question",{"text":112,"@type":113},"To determine how lidocaine affects oral cancer-related cell behaviors in the perioperative context, focusing on proliferation, autophagy, and cellular impedance signals.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"Which experimental methods were used to assess lidocaine’s effects?",{"text":117,"@type":113},"The study used cell viability assays, flow cytometry, real-time PCR, western blotting, electric cell-substrate impedance sensing, and RNA-sequencing analyses.",{"name":119,"@type":110,"acceptedAnswer":120},"How did lidocaine’s effects differ between OECM-1 and SG cells?",{"text":121,"@type":113},"In OECM-1 cells, lidocaine suppressed metabolic activity and proliferation and altered mitochondrial potential while inducing hypoxia and autophagy. In SG cells, lidocaine produced different responses, shifting cell-cycle profiles, increasing apoptotic cells and cytosolic ROS, and changing mRNA expression.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},352145,1790152924,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":24,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":56,"language":139,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":140,"faqs":141,"seo_title":142,"seo_description":67,"update_tm":143,"read_time":144},962090883568,"https://ap-avatar.wpscdn.com/davatar_a8503ba1806abce46bf441b54a3ca4cd","Huang et al. BMCOral Health (2026) 26:1648 BMC Oral Health  \n[https://doi.org/10.1186/s12903-026-08877-4](https://doi.org/10.1186/s12903-026-08877-4)  \nRESEARCH Open Access  \nMolecular-level insights into the therapeutic  potential of lidocaine: effects on proliferation, autophagy, and cellular impedance in human oral cells  \nWei-Zhi Huang1,2, Gunng-Shinng Chen1,3, Shu-Ting Liu4, Shiao-Pieng Lee1,3, Shih-Ming Huang4 and Jia-Lin Chen5,6*  \nAbstract  \nBackground Local anesthesia is widely used in dental treatments such as extractions, implants, gum grafts, and jaw surgery. Recent studies have examined the effects of anesthetics, particularly lidocaine, on postoperative outcomesin patients with cancer; however, its specific role in cancer progression in the perioperative context remains to be explored.  \nMethods In this study, we aimed to explore the effects of lidocaine on oral epidermoid carcinoma meng-1 (OECM- 1) and gingival epithelial Smulow–Glickman (SG) cells using cell viability, flow cytometry, real-time polymerase chain reaction, western blotting, Electric Cell-Substrate Impedance Sensing, and RNA-sequencing analyses.  \nResults Our findings revealed contrasting effects of lidocaine on these cell types. In OECM-1 cells, lidocaine suppressed metabolic activity (> 4 mM), cellular proliferation, S-phase population, and late apoptosis (\u003C 4 mM) . It also altered mitochondrial membrane potential, induced hypoxia and autophagy, and affected the expression of specific proteins and mRNAs. In contrast, SG cells exhibited different responses, with lidocaine influencing cell cycle profiles; increasing the number of apoptotic cells, cytosolic reactive oxygen species (ROS), and JC-1 aggregates; and altering mRNA expression. Additionally, the combination index analysis showed that lidocaine worked synergistically with cisplatin and 5-fluorouracil in both OECM-1 and SG cells. The cellular impedance patterns indicated similarities between the effects of lidocaine and those of lidocaine and cisplatin combination therapy.  \nConclusion In this study, we identified significant differences between OECM-1 and SG cells in terms of the effects of lidocaine on cell cycle profiles, cytosolic ROS, and mitochondrial potential. These results suggest that local anesthetics, such as lidocaine, may play crucial roles in combination therapies for oral cancers and in wound healing in post-gum grafts beyond their conventional use in pain management.  \nKeywords Autophagy, Combination therapy, Cytotoxicity, Cellular impedance, Lidocaine, Mitochondrial membrane potential, Oral cells, Reactive oxygen species  \n*Correspondence:  \nJia-Lin Chen [babe.ane@gmail.com](babe.ane@gmail.com)  \nFull list of author information is available at the end of the article  \n© The Author(s) 2026. Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit [http://creati](http://creati)[vecommons.org/licenses/by-nc-nd/4.0/](vecommons.org/licenses/by-nc-nd/4.0/.)[.](vecommons.org/licenses/by-nc-nd/4.0/.)  \nHuang et al. BMC Oral Health (2026) 26:1648  \nIntroduction  \nLocal anesthetics eff","cbCaiic4Jrq42StC","https://ap.wps.com/l/cbCaiic4Jrq42StC","pdf",9182011,"English","# Background\n# Methods\n# Results\n# Conclusion\n# Introduction","[{\"question\":\"What was the main goal of the study on lidocaine?\",\"answer\":\"To determine how lidocaine affects oral cancer-related cell behaviors in the perioperative context, focusing on proliferation, autophagy, and cellular impedance signals.\"},{\"question\":\"Which experimental methods were used to assess lidocaine’s effects?\",\"answer\":\"The study used cell viability assays, flow cytometry, real-time PCR, western blotting, electric cell-substrate impedance sensing, and RNA-sequencing analyses.\"},{\"question\":\"How did lidocaine’s effects differ between OECM-1 and SG cells?\",\"answer\":\"In OECM-1 cells, lidocaine suppressed metabolic activity and proliferation and altered mitochondrial potential while inducing hypoxia and autophagy. In SG cells, lidocaine produced different responses, shifting cell-cycle profiles, increasing apoptotic cells and cytosolic ROS, and changing mRNA expression.\"}]","Molecular-level insights into the therapeutic potential of lidocaine: effects on proliferation, autophagy, and cellular impedance in human oral cells | PDF",1790097938,48]