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Normal and cancerous breast and prostate cell lines were treated with exogenous zinc across a time course (T0 and T120). A top-down proteomics workflow combining two-dimensional gel electrophoresis and liquid chromatography-tandem mass spectrometry quantified zinc-driven changes in protein expression. Differentially expressed proteins were identified in MCF-7 and PC3 cells versus their matched untreated normal controls, revealing zinc-responsive candidates and highlighting distinct cancer-specific protein landscapes.",{"@graph":69,"@context":123},[70,84,106],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/molecular-insights-into-the-breast-and-prostate-cancer-cells-in-response-to-the-change-of-extracellular-zinc/329999/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/molecular-insights-into-the-breast-and-prostate-cancer-cells-in-response-to-the-change-of-extracellular-zinc/329999.png","ImageObject",300,407,{"name":92,"@type":93},"Jiven","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-11-06","2026-09-21",true,{"@type":102,"interactionType":103,"userInteractionCount":105},"InteractionCounter",{"@type":104},"ViewAction",14,{"@type":107,"mainEntity":108},"FAQPage",[109,115,119],{"name":110,"@type":111,"acceptedAnswer":112},"What is the main research focus of this study?","Question",{"text":113,"@type":114},"The study investigates molecular changes in breast and prostate cancer cells in response to changes in extracellular zinc.","Answer",{"name":116,"@type":111,"acceptedAnswer":117},"Which experimental approaches were used to detect protein changes?",{"text":118,"@type":114},"Proteomic profiling was performed using a top-down approach with two-dimensional gel electrophoresis followed by liquid chromatography-tandem mass spectrometry.",{"name":120,"@type":111,"acceptedAnswer":121},"How were breast and prostate cells compared in the analysis?",{"text":122,"@type":114},"Cancer cell lines were compared against matched normal epithelial cell lines without zinc treatment at T0, and zinc-treated samples were analyzed across time points including T120.","https://schema.org",{"og:url":83,"og:type":125,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":127,"canonical":83},"index,follow",{"doc_id":129,"site_id":62},329999,1790081574,{"code":4,"msg":5,"data":132},{"doc_id":129,"user_id":133,"nickname":92,"user_avatar":134,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":135,"file_id":136,"file_url":137,"file_type":138,"file_size":139,"view_count":105,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":140,"language":141,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":142,"faqs":143,"seo_title":144,"seo_description":67,"update_tm":145,"read_time":146,"preview_page_count":4},1099513958607,"https://ap-avatar.wpscdn.com/avatar/100002390cf8733938c?x-image-process=image/resize,m_fixed,w_180,h_180&k=1778829742770036399","Hindawi  \nJournal of Oncology  \nVolume 2024, Article ID 9925970, 21 pages [https://doi.org/10.1155/2024/9925970](https://doi.org/10.1155/2024/9925970)  \nResearch Article  \nMolecular Insights into the Breast and Prostate Cancer Cells in Response to the Change of Extracellular Zinc  \nShital K. Barman , 1 Monokesh K. Sen ,2 David A. Mahns ,3 Ming J. Wu , 1 and Chandra S. Malladi 4  \n1 School of Science, Western Sydney University, Locked Bag 1797, Penrith, NSW 2751, Australia  \n2 Charles Perkins Centre, School of Medical Sciences, Faculty of Medicine and Health, Te University of Sydney,  \nCamperdown 2006, NSW, Australia  \n3 School of Medicine, Western Sydney University, Locked Bag 1797, Penrith, NSW 2751, Australia  \n4 Proteomics and Lipidomics Lab, School of Medicine, Western Sydney University, Locked Bag 1797, Penrith, NSW 2751, Australia Correspondence should be addressed to Ming J. Wu; [m.wu@westernsydney.edu.au and Chandra](m.wu@westernsydney.edu.au and Chandra) S. Malladi;  \n[c.malladi@westernsydney.edu.au](c.malladi@westernsydney.edu.au)  \nReceived 28 August 2023; Revised 29 November 2023; Accepted 18 December 2023; Published 12 January 2024  \nAcademic Editor: Yuan Seng Wu  \nCopyright © 2024 Shital K. Barman et al. Tis is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.  \nZinc dyshomeostasis is manifested in breast and prostate cancer cells. Tis study attempted to uncover the molecular details prodded by the change of extracellular zinc by employing a panel of normal and cancerous breast and prostate cell lines coupled with the top-down proteomics with two-dimensional gel electrophoresis followed by liquid chromatography-tandem mass spectrometry. Te protein samples were generated from MCF-7 breast cancer cells, MCF10A normal breast cells, PC3 prostate cancer cells, and RWPE-1 normal prostate cells with or without exogenous zinc exposure in a time course ( T0 and T120) . By comparing the cancer cells vs respective normal epithelial cells without zinc treatment ( T0), diferentially expressed proteins (23 upregulated and 18 downregulated in MCF-7 cells; 14 upregulated and 30 downregulated in PC3 cells) were identifed, which provides insights into the intrinsic diferences of breast and prostate cancer cells. Te dynamic protein landscapes in the cancer cells prodded by the extracellular zinc treatment reveal the potential roles of the identifed zinc-responsive proteins (e.g., triosephosphate isomerase, S100A13, tumour proteins hD53 and hD54, and tumour suppressor prohibitin) in breast and prostate cancers. Tis study, for the frst time, simultaneously investigated the two kinds of cancer cells related to zinc dyshomeostasis, and the fndings shed light on the molecular understanding of the breast and prostate cancer cells in response to extracellular zinc variation.  \n1. Introduction  \nZinc (Zn2+) is essential to life. It functions in the cell asa cofactor for well over 300 enzymes and as a structural compon∼ent for approximately 10% of the human pro  \nteome ( 3000 proteins) [1] . Consequently, the cell has developed an elaborate molecular network over the extensive evolutionary timeline to maintain zinc homeostasis. Any disruption of such a network may lead to zinc dyshomeostasis, resulting in health problems such as cancers. Breast cancer is the most common malignancy in  \nfemales worldwide [2, 3], and prostate cancer in males is the second and ffth highest in incidence and mortality, respectively [2] . Both breast and prostate cancers are associated with intracellular zinc dysregulation. Breast cancer cells exhibit elevated intracellular zinc levels compared to their normal epithelial cells [4], while prostate cancer cells show decreased intracellular zinc levels compared to their normal counterparts [5] . Such diametrically opposite zinc profles of breast and prostate can","cbCailtgb6wsgE1h","https://ap.wps.com/l/cbCailtgb6wsgE1h","pdf",3578162,21,"English","# Introduction\n# Zinc homeostasis networks (ZIP, ZnT, and metallothionein)\n## Cellular zinc homeostasis and transporters\n## Proteomics as a tool for cancer research","[{\"question\":\"What is the main research focus of this study?\",\"answer\":\"The study investigates molecular changes in breast and prostate cancer cells in response to changes in extracellular zinc.\"},{\"question\":\"Which experimental approaches were used to detect protein changes?\",\"answer\":\"Proteomic profiling was performed using a top-down approach with two-dimensional gel electrophoresis followed by liquid chromatography-tandem mass spectrometry.\"},{\"question\":\"How were breast and prostate cells compared in the analysis?\",\"answer\":\"Cancer cell lines were compared against matched normal epithelial cell lines without zinc treatment at T0, and zinc-treated samples were analyzed across time points including T120.\"}]","Molecular Insights into the Breast and Prostate Cancer Cells in Response to the Change of Extracellular Zinc | PDF",1789986490,53]