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This study cloned and characterized full-length goose IGF2BP2 cDNA and genomic DNA, revealing conserved RNA-binding domains, 12 exons, 60 variants, and a frameshift-causing deletion (g.2299delG). Expression was elevated in liver, heart, and muscle across embryonic, growing, and laying stages. Overexpression in skeletal muscle satellite cells altered muscle-development and signaling genes, and protein–protein interaction analysis suggested coordinated regulation.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/molecular-characterization-and-functional-insights-into-goose-igf2bp2-during-skeletal-muscle-development/464853/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/molecular-characterization-and-functional-insights-into-goose-igf2bp2-during-skeletal-muscle-development/464853.png","ImageObject",300,407,{"name":92,"@type":93},"Liam","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-04","2026-09-30",true,{"@type":102,"interactionType":103,"userInteractionCount":81},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What is the main focus of this study on goose IGF2BP2?","Question",{"text":112,"@type":113},"The study examines how IGF2BP2 contributes to skeletal muscle development in geese by characterizing the gene and testing functional effects in muscle-related cells.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"What genetic and sequence features of goose IGF2BP2 were identified?",{"text":117,"@type":113},"The authors cloned full-length IGF2BP2 cDNA and genomic DNA, reporting a 2957 bp cDNA with a 1662 bp open reading frame and genomic organization of 12 exons and 11 introns, along with 60 variants including g.2299delG.",{"name":119,"@type":110,"acceptedAnswer":120},"How did IGF2BP2 overexpression affect skeletal muscle satellite cells?",{"text":121,"@type":113},"Overexpression in skeletal muscle satellite cells produced significant changes in genes linked to muscle development and signaling pathways, including upregulation of several factors (e.g., IGF1, EGFR, FGF19) and downregulation of multiple others, with associated pathway regulation inferred from interaction analyses.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},464853,1790811959,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":81,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145},8796095461564,"https://ap-avatar.wpscdn.com/davatar_155a257f0dc6eb9ab79c44ca47cae57d","Article  \nMolecular Characterization and Functional Insights into Goose IGF2BP2 During Skeletal Muscle Development  \nCui Wang 1,†, Yi Liu 1,†, Jiuli Dai 2, Shufang Chen 2 and Daqian He 1, *  \nAcademic Editor: Minli Yu  \nReceived: 26 November 2025  \nRevised: 19 December 2025  \nAccepted: 23 December 2025  \nPublished: 24 December 2025  \nCopyright: © 2025 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.  \n1 Institute of Animal Science and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China; [cuiwang518@saas.sh.cn](cuiwang518@saas.sh.cn) (C.W.); [liuyi20031194@163.com](liuyi20031194@163.com) (Y.L.)  \n2 Ningbo Academy of Agricultural Sciences, Ningbo 315040, China; [13858357201@163.com](13858357201@163.com) (J.D.); [13606780161@163.com](13606780161@163.com) (S.C.)  \n* Correspondence: [shwaterfowl@163.com](shwaterfowl@163.com)  \n† These authors contributed equally to this work.  \nSimple Summary  \nThis study investigates the role of insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2) in geese. We found that IGF2BP2 is highly expressed in muscle tissues and influences key cellular processes. By increasing its activity in muscle cells, we demonstrated that IGF2BP2 may regulate a network of genes critical for muscle development. Our findings indicate that IGF2BP2 acts as an important regulator of muscle development in geese.  \nAbstract  \nInsulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2) is an RNA-binding protein known to play critical roles in metabolism, cell proliferation, and tumorigenesis. Although its involvement in muscle development has been documented in several species, the function of goose IGF2BP2 remains largely unexplored. In this study, we cloned and characterized the full-length cDNA and genomic DNA sequences of goose IGF2BP2 . The cDNA is 2957 bp in length and contains a 1662 bp open reading frame encoding a 553-amino acid protein with five conserved RNA-binding domains. The genomic sequence spans 12,183 bp and consists of 12 exons and 11 introns. A total of 60 genetic variants were identified, including a deletion of a G base at position 2299 (g.2299delG) that results in aframeshift mutation. Expression analysis revealed high levels of IGF2BP2 mRNA in the liver, heart, and muscle tissues of female geese across embryonic (E25d), growing (A70d), and laying (L270d) stages, consistent with a potential role in muscle development (p \u003C 0.05) . Functionally, overexpression of IGF2BP2 in skeletal muscle satellite cells (SMSCs) was associated with significant changes in the expression of several genes linked to muscle development and signaling pathways, including upregulation of IGF1, EGFR, FGF19, BMP6, BMP2, ACVR1C and WNT5A and downregulation of MYBPC3, NODAL, HOXD13, TNXB, and ADD2 (Padj \u003C 0.01) . Furthermore, protein–protein interaction (PPI) network analysis of these genes suggests that IGF2BP2 may coordinate key genes, contributing to its potential role in skeletal muscle development in geese.  \nKeywords: goose; IGF2BP2; expression profile; SMSCs; overexpression; RNA-seq  \n1. Introduction  \nInsulin-like growth factor 2 mRNA-binding proteins (IGF2BPs or IMPs) constitute a conserved family of RNA-binding proteins with three known members: IGF2BP1, IGF2BP2  \nand IGF2BP3 . These proteins play pivotal roles in post-transcriptional gene regulation, critically influencing cellular processes such as proliferation, differentiation, migration, and metabolism [1–3] . As a core member of this family, IGF2BP2 functions as an N6-methyladenosine (m6A) reader, modulating the stability, localization, and translation of target mRNAs involved in metabolic and developmental pathways [4] .  \nThe role of IGF2BP2 in myogenesis is increasingly recognized across species. In mice, IGF2BP2 promotes myoblast proliferation by binding to and stabilizing key transc","cbCaisH038xro2Rq","https://ap.wps.com/l/cbCaisH038xro2Rq","pdf",4608052,17,"English","# Simple Summary\n# Abstract\n# Keywords\n# 1. Introduction","[{\"question\":\"What is the main focus of this study on goose IGF2BP2?\",\"answer\":\"The study examines how IGF2BP2 contributes to skeletal muscle development in geese by characterizing the gene and testing functional effects in muscle-related cells.\"},{\"question\":\"What genetic and sequence features of goose IGF2BP2 were identified?\",\"answer\":\"The authors cloned full-length IGF2BP2 cDNA and genomic DNA, reporting a 2957 bp cDNA with a 1662 bp open reading frame and genomic organization of 12 exons and 11 introns, along with 60 variants including g.2299delG.\"},{\"question\":\"How did IGF2BP2 overexpression affect skeletal muscle satellite cells?\",\"answer\":\"Overexpression in skeletal muscle satellite cells produced significant changes in genes linked to muscle development and signaling pathways, including upregulation of several factors (e.g., IGF1, EGFR, FGF19) and downregulation of multiple others, with associated pathway regulation inferred from interaction analyses.\"}]","Molecular Characterization and Functional Insights into Goose IGF2BP2 During Skeletal Muscle Development | PDF",1790768805,43]