[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-331589-105":59,"doc-detail-331589-en":131},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":124,"head_meta":126,"extra_data":128,"updated_unix":130},105,"en","mir-373-3p-promotes-aerobic-glycolysis-in-colon-cancer-cells-by-targeting-mfn2","miR-373-3p promotes aerobic glycolysis in colon cancer cells by targeting MFN2","","MicroRNAs (miRNAs) are linked to cancer development and may represent therapeutic targets, yet their mechanisms in cancers remain insufficiently clarified. This study examines miR-373-3p in colon cancer cells and shows that miR-373-3p mimics enhance, while miR-373-3p inhibition suppresses, aerobic glycolysis and cell proliferation. Mechanistically, miR-373-3p downregulates MFN2, a glycolysis-suppressing gene, thereby activating glycolysis and promoting proliferation.",{"@graph":69,"@context":123},[70,84,106],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/mir-373-3p-promotes-aerobic-glycolysis-in-colon-cancer-cells-by-targeting-mfn2/331589/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/mir-373-3p-promotes-aerobic-glycolysis-in-colon-cancer-cells-by-targeting-mfn2/331589.png","ImageObject",300,407,{"name":92,"@type":93},"Evangeline","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-29","2026-09-21",true,{"@type":102,"interactionType":103,"userInteractionCount":105},"InteractionCounter",{"@type":104},"ViewAction",14,{"@type":107,"mainEntity":108},"FAQPage",[109,115,119],{"name":110,"@type":111,"acceptedAnswer":112},"What role does miR-373-3p play in colon cancer cells?","Question",{"text":113,"@type":114},"miR-373-3p promotes aerobic glycolysis and proliferation in colon cancer cells, while inhibiting miR-373-3p suppresses both processes.","Answer",{"name":116,"@type":111,"acceptedAnswer":117},"How does miR-373-3p regulate aerobic glycolysis?",{"text":118,"@type":114},"miR-373-3p inhibits MFN2 expression; since MFN2 suppresses glycolysis, its downregulation activates glycolysis and supports proliferation.",{"name":120,"@type":111,"acceptedAnswer":121},"What do the in vivo and clinical observations indicate?",{"text":122,"@type":114},"In a nude mouse tumor model, miR-373-3p increases tumor growth via enhanced lactate formation, and miR-373-3p antagomir blunts tumor growth by reducing lactate production; in human colon cancers, miR-373-3p is elevated while MFN2 mRNA is decreased, with inverse association.","https://schema.org",{"og:url":83,"og:type":125,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":127,"canonical":83},"index,follow",{"doc_id":129,"site_id":62},331589,1790527251,{"code":4,"msg":5,"data":132},{"doc_id":129,"user_id":133,"nickname":92,"user_avatar":134,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":135,"file_id":136,"file_url":137,"file_type":138,"file_size":139,"view_count":105,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":140,"language":141,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":142,"faqs":143,"seo_title":144,"seo_description":67,"update_tm":145,"read_time":146},13056703019662,"https://ap-avatar.wpscdn.com/avatar/be000253a8e92610077?_k=1778726343310543188","Original Article  \nmiR-373-3p promotes aerobic glycolysis in colon cancer cells by targeting MFN2  \nYu Wang 1,2,†, Jie Lun 1,3,†, Yuying Zhang4, Mengchao Yu5, Xingqian Liu 1,2, Jing Guo 1, Hongwei Zhang6, Wensheng Qiu 1, *, and Jing Fang 1, *  \n1 Department of Oncology, the Affiliated Hospital of Qingdao University, Qingdao University, Qingdao Cancer Institute, Qingdao 266071, China, 2School of Basic Medicine, Qingdao University, Qingdao 266071, China, 3School of Pharmacy, Qingdao University, Qingdao 266071, China, 4School of Public Health, Qingdao University, Qingdao 266071, China, 5Central Laboratory, Qingdao Municipal Hospital, Qingdao 266071, China,  \nand 6Shandong Provincial Maternal and Child Health Care Hospital Affiliated to Qingdao University, Jinan 250014, China †These authors contributed equally to this work.  \n*Correspondence address. Tel: +86-13806424200; E-mail: [jfang@qdu.edu.cn](jfang@qdu.edu.cn) (J. F. ) / Tel: +86-17853299199; [E-mail:](E-mail: wsqiuqd@163.com)[ wsqiuqd@163.com](E-mail: wsqiuqd@163.com) (W. Q. ) Received 4 January 2024 Accepted 18 March 2024  \nAbstract  \nMicroRNAs (miRNAs) are implicated in the development of cancers and may serve as potential targets for therapy. However, the functions and underlying mechanisms of miRNAs in cancers are not well understood. This work aims to study the role of miR-373-3p in colon cancer cells. We find that the expression of miR-373-3p mimics promotesand the miR-373-3p inhibitor suppresses aerobic glycolysis and proliferation of colon cancer cells. Mechanistically, miR-373-3p inhibits the expression of MFN2, a gene that is known to suppress glycolysis, which leads to the activation of glycolysis and eventually the proliferation of cells. In a nude mouse tumor model, the expression of miR-373-3p in colon cancer cells promotes tumor growth by enhancing lactate formation, which is inhibited by the co-expression of MFN2 in the cells. Administration of the miR-373-3p antagomir blunts in vivo tumor growth by decreasing lactate production. In addition, in human colon cancers, the expression levels of miR-373-3p are increased, while those of MFN2 mRNA are decreased, and the increase of miR-373-3p is associated with the decrease of MFN2 mRNA. Our results reveal a previously unknown function and underlying mechanism of miR-373-3p in the regulation of glycolysis and proliferation in cancer cells and underscore the potential of targeting miR-373-3p for colon cancer treatment.  \nKey words miRNA, colon cancer, glycolysis, cell proliferation, miR-373-3p, MFN2  \nIntroduction  \nMicroRNAs (miRNAs) are a subclass of small noncoding RNAs (ncRNAs) . They are single-stranded RNA molecules of approximately 19‒24 nucleotides (nt) that are typically derived from 60-to 110-nt RNA hairpin precursors [1] . miRNAs are transcribed as primary miRNAs (pri-miRNAs), which are subsequently cleaved into precursor miRNAs (pre-miRNAs) and further processed into mature single-stranded ~22-nt miRNAs [2] . The biogenesis of miRNAs involves a complex protein system that includes the RNase III enzymes DROSHA and DICER1 [3] . The classic function of miRNAs is post-transcriptionally repressing expressions of specific target proteins by either promoting mRNA decay or dampening translation [1,4] . Recent studies have demonstrated that miRNAs may also be involved in translational upregulation, epigenetic  \nregulation, and transcriptional activation [5] .  \nmiRNAs are involved in biological processes, including cell proliferation, differentiation, and apoptosis [1], and are expressed in distinct spatial and temporal patterns, both during embryonic and postnatal development and in adult tissues [6] . miRNAs have regulatory effects on metabolic enzymes, signaling pathways, and transcription factors involved in glucose and lipid metabolism [7,8] . miRNAs are associated with cancers [9,10] and have been proven to drive or repress tumorigenesis [11] . In cancer cells, miRNAs were found to control the Warburg effec","cbCaintpSjFnUx5A","https://ap.wps.com/l/cbCaintpSjFnUx5A","pdf",2637940,11,"English","# Abstract\n## Introduction\n## Materials and Methods","[{\"question\":\"What role does miR-373-3p play in colon cancer cells?\",\"answer\":\"miR-373-3p promotes aerobic glycolysis and proliferation in colon cancer cells, while inhibiting miR-373-3p suppresses both processes.\"},{\"question\":\"How does miR-373-3p regulate aerobic glycolysis?\",\"answer\":\"miR-373-3p inhibits MFN2 expression; since MFN2 suppresses glycolysis, its downregulation activates glycolysis and supports proliferation.\"},{\"question\":\"What do the in vivo and clinical observations indicate?\",\"answer\":\"In a nude mouse tumor model, miR-373-3p increases tumor growth via enhanced lactate formation, and miR-373-3p antagomir blunts tumor growth by reducing lactate production; in human colon cancers, miR-373-3p is elevated while MFN2 mRNA is decreased, with inverse association.\"}]","miR-373-3p promotes aerobic glycolysis in colon cancer cells by targeting MFN2 | PDF",1789994208,28]