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Using GEO datasets and expression validation by qRT-PCR, the study links miR-3195 with SOX4 and tests functional effects on proliferation, migration, invasion, and apoptosis, supporting miR-3195/SOX4 as a therapeutic 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is the main focus of the study on osteosarcoma?","Question",{"text":62,"@type":63},"The study examines whether miR-3195 suppresses malignant progression in osteosarcoma cells by targeting SOX4 and influencing key cellular behaviors such as proliferation, migration, invasion, and apoptosis.","Answer",{"name":65,"@type":60,"acceptedAnswer":66},"How were miR-3195 and SOX4 relationships investigated?",{"text":67,"@type":63},"The relationship was analyzed using GEO datasets for differential expression, validated with qRT-PCR, and assessed between miR-3195 and the SOX4 3’-UTR using Pearson correlation and a dual-luciferase reporter gene assay.",{"name":69,"@type":60,"acceptedAnswer":70},"What were the observed effects of miR-3195 overexpression in OS cells?",{"text":71,"@type":63},"Overexpression of miR-3195 significantly inhibited proliferation, migration, and invasiveness while promoting apoptosis, and these effects were reversed by increasing SOX4 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\n[https://doi.org/10.1186/s13018-023-04321-3](https://doi.org/10.1186/s13018-023-04321-3)  \nJournal of Orthopaedic Surgery and Research  \n RESEARCH ARTICLE Open Access  \nmiR-3195 suppresses the malignant  \nprogression of osteosarcoma cells via targeting SOX4  \nJianwei Liang1, Dandan Bao2, ZhanYe1, Binhao Cao1, Guojun Jin1, Zhenyu Lu 1 and Jianjun Chen1*  \nAbstract  \nBackground Osteosarcoma (OS) is a highly invasive primary malignancy of the bone that is common in children and adolescents. MicroRNAs (miRNAs) are novel diagnostic and predictive biomarkers for cancers. The miRNA miR- 3195 is aberrantly expressed in multiple types of tumors. However, the expression levels and biological functions of miR-3195 in OS remain unclear.  \nMethods Two Gene Expression Omnibus (GEO) datasets (GSE69470 and GSE16088) were used to analyze differentially expressed miRNAs and mRNAs in osteosarcoma cell lines and OS tissues. Quantitative RT-PCR was used to detect the expression levels of miR-3195 and the SRY-box transcription factor 4 (SOX4) mRNA in OS tissues and cell lines. The relationship between miR-3195 and the 3’-upstream region (3’-UTR) in the SOX4 mRNA (predicted through bioinformatics) was analyzed using Pearson’s correlation analysis and confirmed by a dual-luciferase reporter gene experiment. Cell counting kit-8 assays, colony formation assays, flow cytometry, wound healing assays, transwell assays, and western blotting were performed to explore the effects of miR-3195 levels on SOX4 affected OS cell biological behavior.  \nResults Our results revealed that miR-3195 was the most down-regulated miRNA and SOX4 was the most upregulated mRNA by Bioinformatic analysis. It was further confirmed miR-3195 had low expression, and SOX4 had high expression levels in clinical OS tissue samples; the expression levels of both genes were negatively correlated with each other in OS tissues. Overexpression of miR-3195 in OS cell lines significantly inhibited cell proliferation, migration, and invasiveness, while promoting apoptosis; all these effects were reversed by increasing SOX4 expression levels. We also found that miR-3195 could directly bind with the SOX4 gene and down-regulate SOX4 expression. Conclusions miR-3195 can modulate proliferation, migration, invasiveness, and apoptosis in OS cells by regulating the SOX4 gene. Thus, the miR-3195/SOX4 signaling may be a novel therapeutic target in OS treatment.  \nKeywords miR-3195, SRY-box transcription factor 4, Osteosarcoma, Proliferation, Migration, Invasiveness, Apoptosis  \n*Correspondence: Jianjun Chen [tzfphcjj@sina.com](tzfphcjj@sina.com)  \n1 Department of Orthopedics, The First People’s Hospital of Taizhou, No.218 Hengjie Road, Huangyan District, Taizhou City 318020, Zhejiang Province, China  \n2 Department of Pharmacy, The First People’s Hospital of Taizhou, No.218 Hengjie Road, Huangyan District, Taizhou City 318020, Zhejiang Province, China  \nIntroduction  \nOsteosarcoma (OS) is a highly invasive primary malignancy of the bone that is common in children and young adults; this cancer is also prone to recurrence after therapy [1] . Although great strides have been made over the last few decades in treating OS with primary chemotherapy, tumor excision, and multi-agent adjuvant chemotherapy, patients with recurrent tumorsand distant metastases have low survival rates; this is  \n© The Author(s) 2023. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. I","cbCaivfXwogVxBen","https://ap.wps.com/l/cbCaivfXwogVxBen","pdf",5204091,11,"English","# Abstract\n## Background\n## Methods\n## Results\n## Conclusions\n# Keywords\n# Introduction","[{\"question\":\"What is the main focus of the study on osteosarcoma?\",\"answer\":\"The study examines whether miR-3195 suppresses malignant progression in osteosarcoma cells by targeting SOX4 and influencing key cellular behaviors such as proliferation, migration, invasion, and apoptosis.\"},{\"question\":\"How were miR-3195 and SOX4 relationships investigated?\",\"answer\":\"The relationship was analyzed using GEO datasets for differential expression, validated with qRT-PCR, and assessed between miR-3195 and the SOX4 3’-UTR using Pearson correlation and a dual-luciferase reporter gene assay.\"},{\"question\":\"What were the observed effects of miR-3195 overexpression in OS cells?\",\"answer\":\"Overexpression of miR-3195 significantly inhibited proliferation, migration, and invasiveness while promoting apoptosis, and these effects were reversed by increasing SOX4 expression.\"}]","miR-3195 suppresses the malignant progression of osteosarcoma cells via targeting SOX4 | PDF",1790261481,28]