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This study enrolled 100 STB patients and 100 healthy controls to identify differential microRNAs, validate them through RT-qPCR, and relate miR-29a-3p levels to clinical features and diagnostic accuracy using correlation tests and ROC 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tuberculosis?",{"text":72,"@type":64},"miR-29a-3p was significantly lower in STB patients, and ROC results indicated diagnostic performance (AUC = 0.864), with further improvement when combined with conventional tests and indicators.","https://schema.org",{"og:url":32,"og:type":75,"og:title":10,"og:site_name":45,"og:description":12},"article",{"robots":77,"canonical":32},"index,follow",{"doc_id":79,"site_id":7},442630,1790825461,{"code":4,"msg":82,"data":83},"success",[84,88,92,96,101,106,110,115,120,123,127],{"id":22,"doc_module":4,"doc_module_name":25,"category_name":85,"show_sort_weight":86,"slug":87},"Story & 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BMC Musculoskeletal Disorders (2026) 27:4  \n[https://doi.org/10.1186/s12891-025-09374-5](https://doi.org/10.1186/s12891-025-09374-5)  \nBMC Musculoskeletal Disorders  \nRESEARCH Open Access  \nmiR-29a-3p serves as a potential biomarker  for diagnosing spinal tuberculosis: a comprehensive analysis integrating clinical data and molecular markers  \nMengqi Zhu 1,2†, Rong Fu4†, YuXing Gao 1,2, Linan Wang 1,2 and NingKui Niu 1,3*  \nAbstract  \nBackground Spinal tuberculosis (STB), the most common bone and joint tuberculosis, often causes spinal deformity and impairs quality of life. While microRNAs (miRNAs) regulate various disease pathogenesis, their expression and function in STB remain poorly understood.  \nMethods 100 STB patients (observation group) from Ningxia Medical University Affiliated Hospital (Jan 2021-Dec 2024) and 100 healthy controls were enrolled. miRNA high-throughput sequencing of 3 samples per group identified differential ly expressed miRNAs, validated via known STB signaling pathways. Real-time fluorescence quantitative reverse transcription PCR(RT-qPCR) quantified peripheral blood miR-29a-3p in all participants. Clinical data were collected. Independent samples t-tests, linear regression, Spearman correlation, and receiver operating characteristic(ROC) curves analyzed miR-29a-3p’s expression differences, associations with clinical parameters, and diagnostic performance (alone or combined with conventional indicators) .  \nResults Peripheral blood miR-29a-3p was significantly lower in STB patients (AUC = 0 . 864) . It negatively correlated with erythrocyte sedimentation rate(ESR) and C-reactive protein(CRP) but not with white blood cell count, disease duration, or neutrophil percentage. Combining miR-29a-3p with bacteriological testing, T-cell spot test(T-SPOT), and imaging significantly improved diagnostic performance.  \nConclusion miR-29a-3p shows distinct low expression in STB patients’ peripheral blood, associated with inflammation activity and conventional diagnostic indicators, making it a promising auxiliary biomarker. Critically, integrating miR-29a-3p into combined detection strategies enhances and optimizes current STB diagnostic frameworks, addressing unmet clinical needs for more accurate and efficient diagnosis, thus holding substantial value for improving STB management.  \nKeywords Spinal tuberculosis, MiRNA-seq, MiR-29a-3p, Biomarker  \n†Mengqi Zhu and Rong Fu contributed equally to this work.  \n*Correspondence:  \nNingKui Niu  \n[niuningkui6743242@163.com](niuningkui6743242@163.com)  \n1Department of Orthopedic, General Hospital of Ningxia Medical University, Ningxia Hui Autonomous Region, No.804 Shengli Street, Yinchuan 750004, China  \n2Ningxia Medical University, Ningxia Hui Autonomous Region, Yinchuan 750004, China  \n3Research Center for Prevention and Control of Bone and Joint Tuberculosis, General Hospital of Ningxia Medical University, Yinchuan, Ningxia 750004, China  \n4Shenyang Medical College, Shenyang 110034, China  \n© The Author(s) 2025. Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence,","cbCaiglihXY3wXuE","https://ap.wps.com/l/cbCaiglihXY3wXuE","pdf",2222819,11,"English","# Abstract\n# Introduction\n## Background of spinal tuberculosis\n## Role of microRNAs in tuberculosis","[{\"question\":\"What is the main objective of the study on miR-29a-3p?\",\"answer\":\"To evaluate whether peripheral blood miR-29a-3p can serve as an auxiliary biomarker for diagnosing spinal tuberculosis and to examine its relationship with clinical indicators.\"},{\"question\":\"How were miR-29a-3p levels measured in the participants?\",\"answer\":\"Differential microRNAs were identified by high-throughput sequencing, and peripheral blood miR-29a-3p was quantified using RT-qPCR across all enrolled participants.\"},{\"question\":\"What diagnostic value did miR-29a-3p show for spinal tuberculosis?\",\"answer\":\"miR-29a-3p was significantly lower in STB patients, and ROC results indicated diagnostic performance (AUC = 0.864), with further improvement when combined with conventional tests and indicators.\"}]","miR-29a-3p serves as a potential biomarker for diagnosing spinal tuberculosis - a comprehensive analysis integrating clinical data and molecular markers | PDF",1790701034,28]