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This study analyzes miR-149-3p levels in lung tissues and cells and evaluates lung cancer cell behaviors using H446/DDP and A549/DDP models. Bioinformatics and confirmation identify TMPRSS4 as a downstream target of miR-149-3p. Western blotting assesses migration, invasion, apoptosis, and TMPRSS4 expression, while a nude-mouse graft model tests tumor growth effects.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/mir-149-3p-targeting-tmprss4-regulates-the-sensitivity-to-cisplatin-to-inhibit-the-progression-of-lung-cancer/331637/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/mir-149-3p-targeting-tmprss4-regulates-the-sensitivity-to-cisplatin-to-inhibit-the-progression-of-lung-cancer/331637.png","ImageObject",300,407,{"name":92,"@type":93},"Genevieve","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-22","2026-09-21",true,{"@type":102,"interactionType":103,"userInteractionCount":39},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"Why is improving cisplatin (DDP) sensitivity important in lung cancer?","Question",{"text":112,"@type":113},"Continuous chemotherapy often leads to DDP resistance in lung cancer cells, which reduces therapeutic efficacy. Improving DDP sensitivity is therefore crucial for better treatment outcomes.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"What role does miR-149-3p play in lung cancer DDP sensitivity?",{"text":117,"@type":113},"miR-149-3p expression is reduced in lung cancer tissues and cells. Overexpression of miR-149-3p inhibits progression, promotes apoptosis, and enhances chemosensitivity to DDP, including in vivo.",{"name":119,"@type":110,"acceptedAnswer":120},"How is TMPRSS4 connected to miR-149-3p and the observed cancer effects?",{"text":121,"@type":113},"Bioinformatics predicts TMPRSS4 as a downstream target of miR-149-3p, which is confirmed experimentally. miR-149-3p negatively regulates TMPRSS4, reducing malignancy-associated characteristics and further improving DDP sensitivity.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},331637,1790269435,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":39,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":41},1374391974585,"https://ap-avatar.wpscdn.com/davatar_276721f389ce27ea32af1340a28f341c","RESEARCH ARTICLE  \nmiR-149-3p targeting TMPRSS4 regulates the sensitivity to cisplatin to inhibit the progression of lung cancer  \nBeibei Qin 1, 2\\#, Dongfang Tang 3\\#, and Mingzhi Zhang 1 ∗  \nLung cancer cells tend to develop resistance to cisplatin (DDP) during continuous chemotherapy, making it crucial to improve DDP sensitivity to enhance therapeutic outcomes. The levels of miR-149-3p in lung tissues and cells, as well as the biological behaviors of lung cancer cells, were analyzed. H446/DDP and A549/DDP cell lines were established to investigate how miR-149-3p affects lung cancer cells’ sensitivity to DDP. Bioinformatics analysis predicted transmembrane serine protease 4 (TMPRSS4) as a downstream target of miR-149-3p, which was subsequently confirmed. Western blot analysis was used to examine proteins related to migration, invasion, apoptosis, and TMPRSS4 expression. Additionally, a subcutaneous graft tumor model in nude mice was created to assess the impact of miR-149-3p on tumor growth. In lung cancer tissues and cells, miR-149-3p expression was reduced, while TMPRSS4 expression was elevated. Overexpression of miR-149-3p inhibited cancer progression, promoted apoptosis, and enhanced the chemosensitivity of lung cancer cells to DDP. Moreover, miR-149-3p negatively regulated TMPRSS4, reducing malignancy-associated characteristics of lung cancer cells and further improving their DDP sensitivity. In vivo, high miR-149-3p expression increased the chemosensitivity of cancer cells. In conclusion, miR-149-3p suppresses the aggressive progression of lung cancer by directly downregulating TMPRSS4 and enhances the responsiveness of lung cancer cells to DDP.  \nKeywords: miR-149-3p, lung cancer, TMPRSS4, DDP sensitivity.  \nIntroduction  \nLung cancer, a frequently diagnosed condition, affected 2.2 million people and resulted in 1.8 million deaths worldwide in 2020 [1, 2] . Lung cancer is divided into two major types: small cell lung cancer (SCLC) and non-small cell lung cancer (NSCLC) [3, 4] . SCLC accounts for approximately 15% of lung cancer cases, while NSCLC makes up 85% . NSCLC has several subtypes [5, 6] and is highly malignant, progressing rapidly. Consequently, most patients are diagnosed at a late stage, for which chemotherapy, particularly platinum-based treatments, is often the preferred option [7, 8] . However, prolonged use of cis-diamminedichloroplatinum (DDP) in chemotherapy leads to drug resistance in lung cancer cells, reducing its therapeutic efficacy [9, 10] . Thus, enhancing the sensitivity of lung cancer cells to DDP is crucial.  \nMicroRNAs (miRNAs), approximately 22–24 nucleotides long, belong to the category of non-coding RNAs. Many studies have shown that miRNAs play key roles in regulating various cellular processes, including apoptosis, proliferation, and disease progression [11, 12] . miRNAs target specific genes by basepairing and binding to the target gene’s 3􀀃 -UTR, resulting in mRNA degradation or translation inhibition [13] . Depending on their regulation in tumors, miRNAs can function as oncogenesor tumor suppressors [14] . miR-149-3p, located on chromosome  \n2q37.3, is encoded by the precursor gene miR-149 . Numerous studies have demonstrated that dysregulation of miR-149-3pis associated with pathological processes in cancer, including tumor cell proliferation, metastasis, and apoptosis [15, 16] . Shenet al. [17] found that miR-149-3p overexpression reduced the proliferation and viability of oralsquamous cell carcinoma cells. Another study [18] demonstrated a link between miR-149-3p and DDP resistance in ovarian cancer cells. However, its role in lung cancer progression and its impact on DDP sensitivity remain unclear.  \nTransmembrane serine protease 4 (TMPRSS4), a type II transmembrane serine protease, is located on chromosome 11q23 .3 (Gene ID: 56649) . It is expressed in several human organs, including the esophagus, stomach, small intestine, colon, bladder, and kidney, though its physiological functi","cbCaik4AaLwoBe6t","https://ap.wps.com/l/cbCaik4AaLwoBe6t","pdf",5362202,12,"English","# Introduction\n# Materials and methods","[{\"question\":\"Why is improving cisplatin (DDP) sensitivity important in lung cancer?\",\"answer\":\"Continuous chemotherapy often leads to DDP resistance in lung cancer cells, which reduces therapeutic efficacy. Improving DDP sensitivity is therefore crucial for better treatment outcomes.\"},{\"question\":\"What role does miR-149-3p play in lung cancer DDP sensitivity?\",\"answer\":\"miR-149-3p expression is reduced in lung cancer tissues and cells. Overexpression of miR-149-3p inhibits progression, promotes apoptosis, and enhances chemosensitivity to DDP, including in vivo.\"},{\"question\":\"How is TMPRSS4 connected to miR-149-3p and the observed cancer effects?\",\"answer\":\"Bioinformatics predicts TMPRSS4 as a downstream target of miR-149-3p, which is confirmed experimentally. miR-149-3p negatively regulates TMPRSS4, reducing malignancy-associated characteristics and further improving DDP sensitivity.\"}]","miR-149-3p targeting TMPRSS4 regulates the sensitivity to cisplatin to inhibit the progression of lung cancer | PDF",1789994396]