[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"doc-seo-450318-105":3,"detail-sidebar-cat-0-en-105":80,"doc-detail-450318-en":130},{"code":4,"msg":5,"data":6},0,"ok",{"site_id":7,"language":8,"slug":9,"title":10,"keywords":11,"description":12,"schema_data":13,"social_meta":73,"head_meta":75,"extra_data":77,"updated_unix":79},105,"en","mini-cutting-protocol-and-anatomical-basis-of-adventitious-rooting-in-ocotea-odorifera-journal-article","Mini-cutting protocol and anatomical basis of adventitious rooting in Ocotea odorifera - Journal article","","Ocotea odorifera, a threatened Atlantic Forest tree, requires efficient propagation to support conservation and restoration. The study tested rooting of 2023 spring, summer, autumn, and winter mini-cuttings treated for ten seconds with hydroalcoholic IBA at 0, 1500, 3000, 4500, and 6000 mg L−1, then grown for ninety days under greenhouse conditions. No season–IBA interaction occurred. Rooting peaked at 68.2% with 3000 mg L−1 IBA, while 6000 mg L−1 increased root number and length. Anatomical observations showed roots formed from the vascular cambium.",{"@graph":14,"@context":72},[15,34,55],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/research-report/","Research & Report",3,{"item":32,"name":10,"@type":21,"position":33},"https://docshare.wps.com/document/mini-cutting-protocol-and-anatomical-basis-of-adventitious-rooting-in-ocotea-odorifera-journal-article/450318/",4,{"url":32,"name":10,"@type":35,"image":36,"author":41,"headline":10,"publisher":44,"fileFormat":47,"inLanguage":8,"description":12,"dateModified":48,"datePublished":49,"encodingFormat":47,"isAccessibleForFree":50,"interactionStatistic":51},"DigitalDocument",{"url":37,"@type":38,"width":39,"height":40},"https://docshare.wps.com/thumbnails/mini-cutting-protocol-and-anatomical-basis-of-adventitious-rooting-in-ocotea-odorifera-journal-article/450318.png","ImageObject",300,407,{"name":42,"@type":43},"Aurelia","Person",{"url":19,"name":45,"@type":46},"DocShare","Organization","application/pdf","2026-10-06","2026-09-30",true,{"@type":52,"interactionType":53,"userInteractionCount":30},"InteractionCounter",{"@type":54},"ViewAction",{"@type":56,"mainEntity":57},"FAQPage",[58,64,68],{"name":59,"@type":60,"acceptedAnswer":61},"What experimental factors were tested in the mini-cutting study?","Question",{"text":62,"@type":63},"The study evaluated mini-cuttings collected across the four seasons of 2023 and treated with five concentrations of indole-3-butyric acid (IBA), using a 4×5 factorial arrangement.","Answer",{"name":65,"@type":60,"acceptedAnswer":66},"Which IBA concentration produced the highest rooting percentage?",{"text":67,"@type":63},"The highest rooting percentage (68.2%) occurred with 3000 mg L−1 IBA.",{"name":69,"@type":60,"acceptedAnswer":70},"Where did the adventitious roots originate anatomically?",{"text":71,"@type":63},"Anatomical analysis showed that adventitious roots originated from the vascular cambium.","https://schema.org",{"og:url":32,"og:type":74,"og:title":10,"og:site_name":45,"og:description":12},"article",{"robots":76,"canonical":32},"index,follow",{"doc_id":78,"site_id":7},450318,1791161309,{"code":4,"msg":81,"data":82},"success",[83,87,91,95,100,105,110,114,119,122,126],{"id":22,"doc_module":4,"doc_module_name":25,"category_name":84,"show_sort_weight":85,"slug":86},"Story & Novel",90,"story-novel",{"id":26,"doc_module":4,"doc_module_name":25,"category_name":88,"show_sort_weight":89,"slug":90},"Literature",80,"literature",{"id":33,"doc_module":4,"doc_module_name":25,"category_name":92,"show_sort_weight":93,"slug":94},"Exam",70,"exam",{"id":96,"doc_module":4,"doc_module_name":25,"category_name":97,"show_sort_weight":98,"slug":99},5,"Comic",60,"comic",{"id":101,"doc_module":4,"doc_module_name":25,"category_name":102,"show_sort_weight":103,"slug":104},6,"Technology",50,"technology",{"id":106,"doc_module":4,"doc_module_name":25,"category_name":107,"show_sort_weight":108,"slug":109},7,"Healthcare",40,"healthcare",{"id":111,"doc_module":4,"doc_module_name":25,"category_name":29,"show_sort_weight":112,"slug":113},8,30,"research-report",{"id":115,"doc_module":4,"doc_module_name":25,"category_name":116,"show_sort_weight":117,"slug":118},9,"Religion & Spirituality",20,"religion-spirituality",{"id":117,"doc_module":4,"doc_module_name":25,"category_name":120,"show_sort_weight":117,"slug":121},"World Cup","world-cup",{"id":123,"doc_module":4,"doc_module_name":25,"category_name":124,"show_sort_weight":123,"slug":125},10,"Lifestyle","lifestyle",{"id":127,"doc_module":4,"doc_module_name":25,"category_name":128,"show_sort_weight":96,"slug":129},19,"General","general",{"code":4,"msg":81,"data":131},{"doc_id":78,"user_id":132,"nickname":42,"user_avatar":133,"doc_module":4,"category_id":111,"category_name":29,"doc_title":10,"doc_description":12,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":30,"is_deleted":4,"is_public":22,"is_downloadable":22,"audit_status":22,"page_count":139,"language":140,"language_code":8,"site_id":7,"html_lang":8,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":12,"update_tm":144,"read_time":145},1099514068365,"https://ap-avatar.wpscdn.com/avatar/10000253d8d9f28188e?_k=1776742907772140068","[www. nature.com/scientificreports](www. nature.com/scientificreports)  \nOPEN  \nMini-cutting protocol and anatomical basis of adventitious rooting in Ocotea odorifera  \nSuelen Caroline Da Luz1􀀍, Giovana Bomfim De Alcantara1,  \nLuciana Lopes Fortes Ribas2, Deborah Das Almas1, Henrique Soares Koehler1 & Katia Christina Zuffellato-Ribas2  \nOcotea odorifera (Vell.) Rohwer (Lauraceae), commonly known as canela-sassafrás, is a tree species native to the Atlantic Forest and valued for its essential oils used in cosmetics, pharmaceuticals, and bioinsecticides. Developing an efficient mini-cutting protocol is fundamental for conservation efforts, as it provides a viable alternative for producing seedlings of this threatened species. This study evaluated the rooting of mini-cuttings collected during the four seasons of 2023 and treated with five concentrations of indole-3-butyric acid (IBA), and investigated anatomical features associated with adventitious root formation. The experiment followed a completely randomized design in a 4 × 5 factorial arrangement, with four replications often mini-cuttings per experimental unit. Mini-cuttings, five centimeters in length and containing a pair of apical leaves, were obtained from established ministock plants and subjected to basal immersion in hydroalcoholic IBA solutions (0, 1500, 3000, 4500, and 6000 mg L ⁻ 1 ) for ten seconds, followed by ninety days in a greenhouse under 25 ± 2 °C and 85% RH. No significant interaction was observed between season and IBA concentration. The highest rooting percentage (68.2%) occurred with 3000 mg L ⁻ 1 IBA, whereas 6000 mg L ⁻ 1 resulted in a greater mean number of roots and greater root length. Spring exhibited the best overall performance, with ninety percent rooting. Anatomical analysis revealed that roots originated from the vascular cambium. Based on these findings, we recommend collecting mini-cuttings in spring and applying 3000 mg L ⁻1 IBA  \nKeywords Vegetative propagation, Cutting, Auxin, Plant production, Macropropagation  \nOcotea odorifera (Vell.) Rohwer, commonly known as canela-sassafrás, is an endemic Brazilian tree species belonging to the Lauraceae family. Due to decades of overexploitation, natural populations have been severely depleted, resulting in its classification as a threatened species1.  \nOcotea odorifera has a predominantly Atlantic Forest distribution, occurring mainly in Mixed Ombrophile’s Forest with Araucaria and in Seasonal Forest formations. The species also occurs in the Pampa biome, although in a more restricted manner. Occurrence records confirm its presence primarily in the states of Espírito Santo, Bahia, Paraná, and Rio Grande do Sul. Belonging to the Lauraceae family, it is an evergreen, secondary tree species that can reach 10 to 28 m in height and up to 120 cm in diameter at maturity. Its flowers are unisexual and arranged in panicles, and its fruits are globose berries, each containing a single seed surrounded by a lignified tegument2.  \nBeyond its ecological significance, O. odorifera has notable economic value. The species produces essential oils widely used in cosmetics, perfumery, and pharmaceuticals3. Safrole, distilled from its trunk, bark, leaves, and roots, is internationally recognized for its application in biodegradable insecticides4,5. Additionally, O. odorifera shows potential in agroforestry systems, particularly as a shade provider for pastures due to its symmetrical canopy, and is suitable for reforestation and ecological restoration projects, capable of producing up to 1.7 tons of biomass per hectare. The bark and flowers possess stimulant, sudorific, and diuretic properties, traditionally used in folk medicine for digestive disorders6,7.  \nDespite these attributes, natural regeneration of O. odorifera is limited. The species is slow-growing, reaching reproductive maturity only after 20 years, with irregular fruiting and recalcitrant seeds exhibiting both embryo  \n1Agricultural Sciences Sector, Dep","cbCaicUJTmW59yn4","https://ap.wps.com/l/cbCaicUJTmW59yn4","pdf",3462640,13,"English","# Introduction\n## Species background and economic/ecological relevance\n## Limits to regeneration and conventional propagation\n# Materials and Methods\n## Experimental design and IBA treatments\n## Mini-cutting collection and greenhouse conditions\n# Results\n## Rooting responses across seasons and IBA doses\n# Anatomical Basis\n## Origin of adventitious roots from vascular cambium\n# Conclusions","[{\"question\":\"What experimental factors were tested in the mini-cutting study?\",\"answer\":\"The study evaluated mini-cuttings collected across the four seasons of 2023 and treated with five concentrations of indole-3-butyric acid (IBA), using a 4×5 factorial arrangement.\"},{\"question\":\"Which IBA concentration produced the highest rooting percentage?\",\"answer\":\"The highest rooting percentage (68.2%) occurred with 3000 mg L−1 IBA.\"},{\"question\":\"Where did the adventitious roots originate anatomically?\",\"answer\":\"Anatomical analysis showed that adventitious roots originated from the vascular cambium.\"}]","Mini-cutting protocol and anatomical basis of adventitious rooting in Ocotea odorifera - Journal article | PDF",1790732861,33]