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This study shows that miR-497 is down-regulated in esophageal cancer specimens and cells. Forced miR-497 expression suppresses proliferation, tube formation, and migration. Mechanistically, miR-497 directly binds the 3′-UTR of QKI, reducing QKI expression, and enhances chemotherapy effects in vitro while preventing tumor growth in vivo, indicating miR-497 suppression increases QKI and therapeutic resistance.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/microrna-497-slows-esophageal-cancer-development-and-reverses-chemotherapy-resistance-through-its-target-qki-research-paper/382732/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/microrna-497-slows-esophageal-cancer-development-and-reverses-chemotherapy-resistance-through-its-target-qki-research-paper/382732.png","ImageObject",300,407,{"name":92,"@type":93},"Melati","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-09-25","2026-09-24",true,{"@type":102,"interactionType":103,"userInteractionCount":14},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What did the study find about miR-497 expression in esophageal cancer?","Question",{"text":112,"@type":113},"miR-497 levels were down-regulated in esophageal cancer specimens and cells compared with controls.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How does miR-497 affect esophageal cancer cell behavior?",{"text":117,"@type":113},"Forced miR-497 expression inhibited cell proliferation, tube formation, and migration in esophageal cancer cells.",{"name":119,"@type":110,"acceptedAnswer":120},"What is the direct molecular target of miR-497 identified in this work?",{"text":121,"@type":113},"miR-497 directly binds the 3′-UTR of QKI, and miR-497 overexpression suppresses QKI expression.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},382732,1790367010,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":14,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":36},962085570644,"https://ap-avatar.wpscdn.com/davatar_994ba38a5ba835b3df7d355c54d3ed8d","[www.aging-us.com](www.aging-us.com) AGING 2023, Vol. 15, No. 9  \nResearch Paper  \nmicroRNA-497 slows esophageal cancer development and reverses chemotherapy resistance through its target QKI  \nYun-Xia Xie1, Zhi-Hao Zhou2, Shu-Wen Liu1, Ye Zhang3, Wen-Jing Liu3, Rui-Ke Zhang1, Ming-Liang He4, Jian-Ge Qiu1, Lin Wang1,2, Bing-Hua Jiang1  \n1Academy of Medical Science, Zhengzhou University, Zhengzhou 450000, China  \n2 BGI College and Henan Institute of Medical and Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450000, China  \n3The Affiliated Cancer Hospital of Zhengzhou University and Henan Cancer Hospital, Zhengzhou University, Zhengzhou 450000, China  \n4 Department of Biomedical Sciences, City University of Hong Kong, Hong Kong 999077, Hong Kong  \nCorrespondence to: Lin Wang, Bing-Hua Jiang; [email:](email: wanglinzz@zzu.edu.cn)[ ](email: wanglinzz@zzu.edu.cn)[wanglinzz@zzu.edu.cn](email: wanglinzz@zzu.edu.cn), [binghjiang@zzu.edu.cn](binghjiang@zzu.edu.cn)[ ](binghjiang@zzu.edu.cn)[Keywords:](Keywords: miRNA-497)[ miRNA-497](Keywords: miRNA-497), QKI, esophageal cancer, chemotherapy resistance, tumor growth  \nReceived: February 2, 2023 Accepted: May 1, 2023 Published: May 11, 2023  \nCopyright: © 2023 Xie et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.  \nABSTRACT  \nEsophageal cancer (EC) is considered one of the most lethal cancers in human beings, and multiple miRNAs have been investigated to be involved in EC development by targeting their target genes. However, the function and related mechanism of miRNA-497 on EC tumorigenesis remain uncertain. This study first demonstrated that the expression levels of miR-497 in esophageal cancer specimens and cells were down-regulated. Forced expression of miR-497 inhibited cell proliferation, tube formation and migration in EC cells. To further investigate the potential molecular mechanism of miR-497 suppression in regulating EC, we found that miR-497 directly binds to the 3'-untranslational region of QKI, miR-497 overexpression suppressed QKI expression. We further found that overexpression of miR-497 enhanced the effect of chemotherapy in EC cell lines, and prevented the tumor growth of EC in vivo. Our findings indicated that miR-497 suppression increased QKI expression and therapeutic resistance of esophageal cancer, which is likely to be a biomarker of EC progression and potential therapeutic target.  \nINTRODUCTION  \nEsophageal cancer is considered one of the most common malignancies in human digestive system [1] . Despite advances in the esophageal cancer management and therapy in patients, the 5-year overall survival rates (~ 10%) and postesophagectomy survival rates (~ 15- 40%) remain quite low [2] . Esophageal cancer is often first diagnosed at advanced clinical stages, due to mostly lack of early clinical obvious symptoms inpatients [3, 4] . Identification of susceptible genes or biomarkers will be valuable to predict the therapy response of EC patients for promoting the overall survival rates of patients. Therefore, investigating new  \nmolecular mechanisms of EC development is urgently needed.  \nMicroRNAs (miRNAs) are noncoding RNAs in cells with 18-25 nucleotide that are highly conserved in humans and are endogenous biological regulators of multiple gene expression [5–7] . MiRNAs typically interact with the 3 ′-UTRs (3 ′-untranslated regions) of target genes ’ mRNAs (messenger RNAs), triggering miRNA cleavage and then degrading the indicated mRNA or inhibiting the translation process. MiRNAsare reported to involved in several cancer development processes, including tumor initiation, metastasis, drug resistance and tumor recurrence [8–10] . In addition,  \nmiRNAs are represented as potential biomarkers for early diagnosis or/and therapeutic targets for treatment of ","cbCainP2A2wEbSQl","https://ap.wps.com/l/cbCainP2A2wEbSQl","pdf",2028867,16,"English","# ABSTRACT\n# INTRODUCTION\n## Esophageal cancer burden and need for biomarkers\n## MicroRNAs and mechanisms in cancer\n## Background on QKI and miR-497-QKI pathway\n## Research questions addressed\n# MATERIALS AND METHODS","[{\"question\":\"What did the study find about miR-497 expression in esophageal cancer?\",\"answer\":\"miR-497 levels were down-regulated in esophageal cancer specimens and cells compared with controls.\"},{\"question\":\"How does miR-497 affect esophageal cancer cell behavior?\",\"answer\":\"Forced miR-497 expression inhibited cell proliferation, tube formation, and migration in esophageal cancer cells.\"},{\"question\":\"What is the direct molecular target of miR-497 identified in this work?\",\"answer\":\"miR-497 directly binds the 3′-UTR of QKI, and miR-497 overexpression suppresses QKI expression.\"}]","microRNA-497 slows esophageal cancer development and reverses chemotherapy resistance through its target QKI - Research Paper | PDF",1790252120]