[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"doc-seo-376369-105":3,"detail-sidebar-cat-0-en-105":79,"doc-detail-376369-en":129},{"code":4,"msg":5,"data":6},0,"ok",{"site_id":7,"language":8,"slug":9,"title":10,"keywords":11,"description":12,"schema_data":13,"social_meta":72,"head_meta":74,"extra_data":76,"updated_unix":78},105,"en","mfsd12-transcriptionally-regulated-by-plagl2-promotes-bladder-cancer-progression-abstract","MFSD12, transcriptionally regulated by PLAGL2, promotes bladder cancer progression - Abstract","","Bladder cancer (BLCA) is a common, life-threatening malignancy, and identifying new molecular signaling targets is crucial. MFSD12 expression is elevated in BLCA, and functional studies using Tet-inducible lentiviral manipulation in UMUC3 and 5637 cells show that MFSD12 knockdown suppresses proliferation, migration, invasion, and G1 cell-cycle progression, reducing lung metastasis and xenografted tumor formation. Transcriptomics/metabolomics plus luciferase and ChIP-PCR indicate MFSD12 is positively regulated by transcription factor PLAGL2, supporting an MFSD12 pro-tumor role in BLCA.",{"@graph":14,"@context":71},[15,34,54],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/healthcare/","Healthcare",3,{"item":32,"name":10,"@type":21,"position":33},"https://docshare.wps.com/document/mfsd12-transcriptionally-regulated-by-plagl2-promotes-bladder-cancer-progression-abstract/376369/",4,{"url":32,"name":10,"@type":35,"image":36,"author":41,"headline":10,"publisher":44,"fileFormat":47,"inLanguage":8,"description":12,"dateModified":48,"datePublished":48,"encodingFormat":47,"isAccessibleForFree":49,"interactionStatistic":50},"DigitalDocument",{"url":37,"@type":38,"width":39,"height":40},"https://docshare.wps.com/thumbnails/mfsd12-transcriptionally-regulated-by-plagl2-promotes-bladder-cancer-progression-abstract/376369.png","ImageObject",300,407,{"name":42,"@type":43},"Fans","Person",{"url":19,"name":45,"@type":46},"DocShare","Organization","application/pdf","2026-09-24",true,{"@type":51,"interactionType":52,"userInteractionCount":26},"InteractionCounter",{"@type":53},"ViewAction",{"@type":55,"mainEntity":56},"FAQPage",[57,63,67],{"name":58,"@type":59,"acceptedAnswer":60},"What role does MFSD12 play in bladder cancer progression?","Question",{"text":61,"@type":62},"MFSD12 promotes bladder cancer progression. Knockdown inhibits proliferation, migration, invasion, and G1 cell-cycle progression, while overexpression enhances malignant phenotypes.","Answer",{"name":64,"@type":59,"acceptedAnswer":65},"How is MFSD12 regulated according to the study?",{"text":66,"@type":62},"MFSD12 is transcriptionally upregulated by PLAGL2. Luciferase reporter and ChIP-PCR assays support positive regulation via PLAGL2.",{"name":68,"@type":59,"acceptedAnswer":69},"What experimental approaches were used to evaluate MFSD12 in BLCA?",{"text":70,"@type":62},"The study used Tet-inducible lentiviral genetic manipulation in BLCA cell lines, followed by treatment conditions, and then transcriptomics/metabolomics. In addition, luciferase reporters and ChIP-PCR were used to assess PLAGL2 regulation, and xenograft/metastasis assessments were performed in vivo.","https://schema.org",{"og:url":32,"og:type":73,"og:title":10,"og:site_name":45,"og:description":12},"article",{"robots":75,"canonical":32},"index,follow",{"doc_id":77,"site_id":7},376369,1790246485,{"code":4,"msg":80,"data":81},"success",[82,86,90,94,99,104,108,113,118,121,125],{"id":22,"doc_module":4,"doc_module_name":25,"category_name":83,"show_sort_weight":84,"slug":85},"Story & Novel",90,"story-novel",{"id":26,"doc_module":4,"doc_module_name":25,"category_name":87,"show_sort_weight":88,"slug":89},"Literature",80,"literature",{"id":33,"doc_module":4,"doc_module_name":25,"category_name":91,"show_sort_weight":92,"slug":93},"Exam",70,"exam",{"id":95,"doc_module":4,"doc_module_name":25,"category_name":96,"show_sort_weight":97,"slug":98},5,"Comic",60,"comic",{"id":100,"doc_module":4,"doc_module_name":25,"category_name":101,"show_sort_weight":102,"slug":103},6,"Technology",50,"technology",{"id":105,"doc_module":4,"doc_module_name":25,"category_name":29,"show_sort_weight":106,"slug":107},7,40,"healthcare",{"id":109,"doc_module":4,"doc_module_name":25,"category_name":110,"show_sort_weight":111,"slug":112},8,"Research & Report",30,"research-report",{"id":114,"doc_module":4,"doc_module_name":25,"category_name":115,"show_sort_weight":116,"slug":117},9,"Religion & Spirituality",20,"religion-spirituality",{"id":116,"doc_module":4,"doc_module_name":25,"category_name":119,"show_sort_weight":116,"slug":120},"World Cup","world-cup",{"id":122,"doc_module":4,"doc_module_name":25,"category_name":123,"show_sort_weight":122,"slug":124},10,"Lifestyle","lifestyle",{"id":126,"doc_module":4,"doc_module_name":25,"category_name":127,"show_sort_weight":95,"slug":128},19,"General","general",{"code":4,"msg":80,"data":130},{"doc_id":77,"user_id":131,"nickname":42,"user_avatar":132,"doc_module":4,"category_id":105,"category_name":29,"doc_title":10,"doc_description":12,"doc_content":133,"file_id":134,"file_url":135,"file_type":136,"file_size":137,"view_count":26,"is_deleted":4,"is_public":22,"is_downloadable":22,"audit_status":22,"page_count":138,"language":139,"language_code":8,"site_id":7,"html_lang":8,"table_of_contents":140,"faqs":141,"seo_title":142,"seo_description":12,"update_tm":143,"read_time":144},5909892330395,"https://ap-avatar.wpscdn.com/davatar_6f874abed73319feea01a86fa6f0fab8","communications biology Article  \n\n| A Nature Portfolio journal |  | |\n| --- | --- | --- |\n| [https://doi.org/10.1038/s42003-025-08801-6](https://doi.org/10.1038/s42003-025-08801-6) |  |  |\n| MFSD12, transcriptionally regulated by PLAGL2, promotes bladder cancer progression\u003Cbr> Check for updates |  |  |\n| Jiani He1, Changming Dong2,3, Xiandong Song2,3,4, Xinyu Bao2, Zhongkai Qiu5, Hao Zhang2,3, Yuanjun Jiang2,3, Tao Liu2,3 & Xiaojun Man 2,3  |  |  |\n| Bladder cancer (BLCA) is one of the most common malignant tumors of the urinary system. Identification of novel molecular signaling targets for the tumorigenesis of BLCA is important. Data from the Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) databases suggest that major facilitator superfamily domain containing12(MFSD12)mayactasan important oncogene in BLCA. MFSD12 expression is confirmed to be elevated in BLCA patients. Genetic manipulation of MFSD12 mediated by Tet-inducible lentiviral expression vector is conducted in two BLCA cell lines, including UMUC3 and 5637 . Following this manipulation, the cells are subjected to treatment with or without doxycycline. Our results show that MFSD12 knockdown inhibits cell proliferation, migration, and invasion, and arrests the G1 stage-induced cell cycle. Furthermore, silencing of MFSD12 reduces lung metastatic lesions and xenografted tumor formation of BLCA cells. To further explore the effect of MFSD12 on BLCA cells, transcriptomics and metabolomics analyses are performed on MFSD12-overexpressing cells. Subsequently, luciferase reporters and chromatin immunoprecipitation (ChIP) -PCR assays reveal that MFSD12 is regulated positively by pleomorphic adenoma gene like-2 (PLAGL2), an important transcription factor. Collectively, our results indicate that MFSD12 exerts a tumor-promoting effect on BLCA progression, under the modulation of transcription factor PLAGL2 . |  |  |\n| Bladder cancer(BLCA)isthe most common malignanttumor ofthe urinary system, with more than 570,000 cases diagnosed globally in 2020, representing a serious threat to public health1. The main histological type of BLCAis transitional cell carcinoma, which accounts for approximately90% of BLCA2. Furthermore, BLCA can be divided into non-muscle-invasive BLCA and muscle-invasive BLCA depending on whether the tumor has invaded the detrusor3. Unfortunately, one-half of patients with muscleinvasive BLCA develop distant metastases and 15% ~ 20% of non-muscle invasive bladder cancer patients progress to muscle-invasive BLCA4. Therefore, exploring new therapeutic targets for BLCA is of great significance.\u003Cbr>The major facilitator superfamily domain containing 12 (MFSD12) encodes a protein involved in the transmembrane transport of cysteine5. Research has shown that MFSD12 promotes the growth and metastasis of melanoma cells, and patients with high MFSD12expression were correlated with worse prognosis6. In non-small cell lung cancer, MFSD12 is | significantly elevated inM2macrophages, suggestingthat high expression of MFSD12 promotes tumorigenesis and is significantly associated with shorter survival7. In addition, MFSD12 can inhibit the proliferation and cycle progression of SH-SY5Y neuroblastoma cells8. These findings reveal that MFSD12 plays distinctive roles in different types of tumors. However, the expression and function ofMFSD12in BLCA are still unclear. Data from the Gene Expression Omnibus (GEO) database (GSE190079) and TCGA databases show that MFSD12 is an important oncogene in BLCA occurrence, and its expression is highly expressed in BLCA. Thus, we speculate that MFSD12 could serve as a potential target in BLCA, which is worth further exploration.\u003Cbr>The pleomorphic adenoma gene (PLAG) family contains 3 transcriptional activators, including pleomorphic adenoma gene like-2 (PLAGL2), PLAG1, and PLAGL19. PLAGL2, as a zinc finger PLAG transcription factor, is aberrantly expressed in several types of malignant tumors10. PLAGL2 affects the malignant behaviors of ","cbCaibt6p0gj3Czt","https://ap.wps.com/l/cbCaibt6p0gj3Czt","pdf",15206165,15,"English","# Results\n## High expression of MFSD12 in BLCA","[{\"question\":\"What role does MFSD12 play in bladder cancer progression?\",\"answer\":\"MFSD12 promotes bladder cancer progression. Knockdown inhibits proliferation, migration, invasion, and G1 cell-cycle progression, while overexpression enhances malignant phenotypes.\"},{\"question\":\"How is MFSD12 regulated according to the study?\",\"answer\":\"MFSD12 is transcriptionally upregulated by PLAGL2. Luciferase reporter and ChIP-PCR assays support positive regulation via PLAGL2.\"},{\"question\":\"What experimental approaches were used to evaluate MFSD12 in BLCA?\",\"answer\":\"The study used Tet-inducible lentiviral genetic manipulation in BLCA cell lines, followed by treatment conditions, and then transcriptomics/metabolomics. In addition, luciferase reporters and ChIP-PCR were used to assess PLAGL2 regulation, and xenograft/metastasis assessments were performed in vivo.\"}]","MFSD12, transcriptionally regulated by PLAGL2, promotes bladder cancer progression - Abstract | PDF",1790218452,38]