[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-386535-105":59,"doc-detail-386535-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","methionine-producing-tumor-microbe-environment-fuels-growth-of-solid-tumors","Methionine-producing tumor micro(be) environment fuels growth of solid tumors","","Recent studies reveal microbes are nearly ubiquitous in solid tumors, and specific bacteria can shape cancer progression. The study proposes that local microbial dysbiosis enables cancer phenotypes by provisioning essential metabolites to tumor cells. Using 16S rDNA sequencing of patient lung samples, lung tumors are enriched for methionine-producing bacteria. Experiments with methionine-dependent E. coli and LUAD cells assess proliferation, cell-cycle effects, death, methylation potential, and xenograft growth under methionine restriction.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/methionine-producing-tumor-microbe-environment-fuels-growth-of-solid-tumors/386535/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/methionine-producing-tumor-microbe-environment-fuels-growth-of-solid-tumors/386535.png","ImageObject",300,407,{"name":92,"@type":93},"Blitz","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-11-03","2026-09-24",true,{"@type":102,"interactionType":103,"userInteractionCount":52},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What central hypothesis links the microbiome to solid tumor growth in this study?","Question",{"text":112,"@type":113},"Local microbial dysbiosis is proposed to enable cancer phenotypes by provisioning essential metabolites, such as methionine, directly to tumor cells under nutrient-restricted conditions.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How was the lung tumor microbiome characterized in the methods?",{"text":117,"@type":113},"The study used 16S rDNA sequencing on 75 patient lung samples to identify bacteria enriched for methionine-producing capabilities.",{"name":119,"@type":110,"acceptedAnswer":120},"What was tested to determine whether tumor cells benefit from bacterial methionine?",{"text":121,"@type":113},"The researchers conditioned cell-culture media with wild-type and methionine-auxotrophic E. coli and measured LUAD proliferation, along with functional assays under methionine restriction.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},386535,1790330893,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":52,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145},24464137899374,"https://us-avatar.wpscdn.com/davatar_3d24733baf745e90a7e4bdd5f77d97b2","Cellular Oncology (2023) 46:1659–1673  \n[https://doi.org/10.1007/s13402-023-00832-7](https://doi.org/10.1007/s13402-023-00832-7)  \nMethionine‑producing tumor micro(be) environment fuels growth of solid tumors  \nAlexis A. Vega1,2 · Erin A. Marshall3,4 · Avery J. C. Noonan5,6 · Fernando Sergio Leitao Filho7 · Julia Yang7 ·  \nGreg L. Stewart3,4 · Fraser D. Johnson3,4 · Emily A. Vucic8 · Michelle E. Pewarchuk3,4 · Parag P. Shah2 · Brian F. Clem1,2 · Corey Nislow9 · Stephen Lam3 · William W. Lockwood3,4,10 · Steven J. Hallam5,6,11,12,13 · Janice M. Leung7 ·  \nLevi J. Beverly2 · Wan L. Lam3,4,10  \nAccepted: 17 May 2023 / Published online: 15 June 2023 © The Author(s) 2023  \nAbstract  \nBackground Recent studies have uncovered the near-ubiquitous presence of microbes in solid tumors of diverse origins. Previous literature has shown the impact of specific bacterial species on the progression of cancer. We propose that local microbial dysbiosis enables certain cancer phenotypes through provisioning of essential metabolites directly to tumor cells. Methods 16S rDNA sequencing of 75 patient lung samples revealed the lung tumor microbiome specifically enriched for bacteria capable of producing methionine. Wild-type (WT) and methionine auxotrophic (metA mutant) E. coli cells were used to condition cell culture media and the proliferation of lung adenocarcinoma (LUAD) cells were measured using SYTO60 staining. Further, colony forming assay, Annexin V Staining, BrdU, AlamarBlue, western blot, qPCR, LINE microarray and subcutaneous injection with methionine modulated feed were used to analyze cellular proliferation, cell-cycle, cell death, methylation potential, and xenograft formation under methionine restriction. Moreover, C 14-labeled glucose was used to illustrate the interplay between tumor cells and bacteria.  \nResults/Discussion Our results show bacteria found locally within the tumor microenvironment are enriched for methionine synthetic pathways, while having reduced S-adenosylmethionine metabolizing pathways. As methionine is one of nine essential amino acids that mammals are unable to synthesize de novo, we investigated a potentially novel function for the microbiome, supplying essential nutrients, such as methionine, to cancer cells. We demonstrate that LUAD cells can utilize methionine generated by bacteria to rescue phenotypes that would otherwise be inhibited due to nutrient restriction. In addition to this, with WT and metA mutant E. coli, we saw a selective advantage for bacteria with an intact methionine synthetic pathway to survive under the conditions induced by LUAD cells. These results would suggest that there is a potential bidirectional cross-talk between the local microbiome and adjacent tumor cells. In this study, we focused on methionine as one of the critical molecules, but we also hypothesize that additional bacterial metabolites may also be utilized by LUAD. Indeed, our radiolabeling data suggest that other biomolecules are shared between cancer cells and bacteria. Thus, modulating the local microbiome may have an indirect effect on tumor development, progression, and metastasis.  \nKeywords Lung adenocarcinoma · Bacteria · Microbiome · Methionine restriction  \n\n| Alexis A. Vega and Erin A. Marshall contributed equally to this work. |\n| --- |\n| Levi J. Beverly and Wan L. Lam are senior author. |\n\nExtended author information available on the last page of the article  \n1 Introduction  \nLung cancer is a leading cause of cancer-related deaths worldwide [1] . Recent studies have demonstrated the importance of interactions between lung tumors and their microenvironment (TME), particularly in the case of immune cells, where the therapeutic implications have profound impactson treatment [2–4] . The tumor microbiome, another important component of the TME, has only recently come into focus despite long recognition of the role of gut microbiota  \nin regulating immune system health and disease [5–7] . The impact of the rich d","cbCaihWnEftraBaR","https://ap.wps.com/l/cbCaihWnEftraBaR","pdf",4605722,15,"English","# Introduction\n## Tumor microenvironment interactions\n## Tumor microbiome and bacterial diversity\n## Bacteria, immune stimulation, and nutritional provisioning","[{\"question\":\"What central hypothesis links the microbiome to solid tumor growth in this study?\",\"answer\":\"Local microbial dysbiosis is proposed to enable cancer phenotypes by provisioning essential metabolites, such as methionine, directly to tumor cells under nutrient-restricted conditions.\"},{\"question\":\"How was the lung tumor microbiome characterized in the methods?\",\"answer\":\"The study used 16S rDNA sequencing on 75 patient lung samples to identify bacteria enriched for methionine-producing capabilities.\"},{\"question\":\"What was tested to determine whether tumor cells benefit from bacterial methionine?\",\"answer\":\"The researchers conditioned cell-culture media with wild-type and methionine-auxotrophic E. coli and measured LUAD proliferation, along with functional assays under methionine restriction.\"}]","Methionine-producing tumor micro(be) environment fuels growth of solid tumors | PDF",1790274154,38]