[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"doc-seo-329583-105":3,"detail-sidebar-cat-0-en-105":81,"doc-detail-329583-en":130},{"code":4,"msg":5,"data":6},0,"ok",{"site_id":7,"language":8,"slug":9,"title":10,"keywords":11,"description":12,"schema_data":13,"social_meta":74,"head_meta":76,"extra_data":78,"updated_unix":80},105,"en","metabolism-and-spatial-transcription-resolved-heterogeneity-of-glutamine-metabolism-in-cervical-carcinoma-research-paper","Metabolism and spatial transcription resolved heterogeneity of glutamine metabolism in cervical carcinoma - Research paper","","Reprogramming of cellular metabolism drives tumor growth and progression, yet high-resolution maps of metabolism and transcription in cervical squamous cell carcinoma remain limited. This study integrates AFADESI-MSI metabolite imaging with spatial transcriptomics to characterize metabolic and gene-expression landscapes in tumor versus normal tissues. Immune histochemistry in 122 cervical cancer and 30 cervicitis cases validates ASCT2 expression, linking region-specific glutamine pathway disruption and heterogeneous glutamate intensity to ASCT2 levels.",{"@graph":14,"@context":73},[15,34,56],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/research-report/","Research & Report",3,{"item":32,"name":10,"@type":21,"position":33},"https://docshare.wps.com/document/metabolism-and-spatial-transcription-resolved-heterogeneity-of-glutamine-metabolism-in-cervical-carcinoma-research-paper/329583/",4,{"url":32,"name":10,"@type":35,"image":36,"author":41,"headline":10,"publisher":44,"fileFormat":47,"inLanguage":8,"description":12,"dateModified":48,"datePublished":49,"encodingFormat":47,"isAccessibleForFree":50,"interactionStatistic":51},"DigitalDocument",{"url":37,"@type":38,"width":39,"height":40},"https://docshare.wps.com/thumbnails/metabolism-and-spatial-transcription-resolved-heterogeneity-of-glutamine-metabolism-in-cervical-carcinoma-research-paper/329583.png","ImageObject",300,407,{"name":42,"@type":43},"Evangeline","Person",{"url":19,"name":45,"@type":46},"DocShare","Organization","application/pdf","2026-11-11","2026-09-21",true,{"@type":52,"interactionType":53,"userInteractionCount":55},"InteractionCounter",{"@type":54},"ViewAction",9,{"@type":57,"mainEntity":58},"FAQPage",[59,65,69],{"name":60,"@type":61,"acceptedAnswer":62},"What methods were used to study glutamine metabolism in cervical cancer tissues?","Question",{"text":63,"@type":64},"AFADESI-MSI was used for airflow-assisted desorption electrospray ionization mass spectrometry imaging, and spatial transcriptomics (ST) was used to quantify mRNA levels. Immune histochemistry was used for clinical validation of ASCT2 expression.","Answer",{"name":66,"@type":61,"acceptedAnswer":67},"What did AFADESI-MSI reveal about glutamine metabolism in different patients and tissues?",{"text":68,"@type":64},"Metabolic differences were detected among cervical squamous cell carcinoma patients, with glutamine-related pathways showing stronger variation. Glutamate intensity was higher in cancerous tissue than normal tissue, and it varied across regions rather than being uniform.",{"name":70,"@type":61,"acceptedAnswer":71},"How is ASCT2 related to metabolic heterogeneity in cervical carcinoma?",{"text":72,"@type":64},"Differential ASCT2 expression (encoded by SLC1A5) across tumor regions was identified as a key factor driving heterogeneity in glutamine metabolism. Higher ASCT2 expression corresponded to higher glutamate intensity in the same region, and ASCT2 protein levels were higher in CSCC tissues than in normal tissues.","https://schema.org",{"og:url":32,"og:type":75,"og:title":10,"og:site_name":45,"og:description":12},"article",{"robots":77,"canonical":32},"index,follow",{"doc_id":79,"site_id":7},329583,1790012807,{"code":4,"msg":82,"data":83},"success",[84,88,92,96,101,106,111,115,119,122,126],{"id":22,"doc_module":4,"doc_module_name":25,"category_name":85,"show_sort_weight":86,"slug":87},"Story & Novel",90,"story-novel",{"id":26,"doc_module":4,"doc_module_name":25,"category_name":89,"show_sort_weight":90,"slug":91},"Literature",80,"literature",{"id":33,"doc_module":4,"doc_module_name":25,"category_name":93,"show_sort_weight":94,"slug":95},"Exam",70,"exam",{"id":97,"doc_module":4,"doc_module_name":25,"category_name":98,"show_sort_weight":99,"slug":100},5,"Comic",60,"comic",{"id":102,"doc_module":4,"doc_module_name":25,"category_name":103,"show_sort_weight":104,"slug":105},6,"Technology",50,"technology",{"id":107,"doc_module":4,"doc_module_name":25,"category_name":108,"show_sort_weight":109,"slug":110},7,"Healthcare",40,"healthcare",{"id":112,"doc_module":4,"doc_module_name":25,"category_name":29,"show_sort_weight":113,"slug":114},8,30,"research-report",{"id":55,"doc_module":4,"doc_module_name":25,"category_name":116,"show_sort_weight":117,"slug":118},"Religion & Spirituality",20,"religion-spirituality",{"id":117,"doc_module":4,"doc_module_name":25,"category_name":120,"show_sort_weight":117,"slug":121},"World Cup","world-cup",{"id":123,"doc_module":4,"doc_module_name":25,"category_name":124,"show_sort_weight":123,"slug":125},10,"Lifestyle","lifestyle",{"id":127,"doc_module":4,"doc_module_name":25,"category_name":128,"show_sort_weight":97,"slug":129},19,"General","general",{"code":4,"msg":82,"data":131},{"doc_id":79,"user_id":132,"nickname":42,"user_avatar":133,"doc_module":4,"category_id":112,"category_name":29,"doc_title":10,"doc_description":12,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":55,"is_deleted":4,"is_public":22,"is_downloadable":22,"audit_status":22,"page_count":139,"language":140,"language_code":8,"site_id":7,"html_lang":8,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":12,"update_tm":144,"read_time":145,"preview_page_count":4},13056703019662,"https://ap-avatar.wpscdn.com/avatar/be000253a8e92610077?_k=1778726343310543188","Liu et al. BMC Cancer (2024) 24:1504  \n[https://doi.org/10.1186/s12885-024-13275-6](https://doi.org/10.1186/s12885-024-13275-6)  \nBMC Cancer  \n RESEARCH Open Access  \nMetabolism and spatial transcription resolved heterogeneity of glutamine metabolism in cervical carcinoma  \nQian Liu 1, Jiayu Zhu1, Guzalinuer Abulizi2* and Ayshamgul Hasim 1*  \nAbstract  \nBackground Reprogramming of cellular metabolism is a pivotal mechanism employed by tumor cells to facilitate cell growth, proliferation, and differentiation, thereby propelling the progression of cancer. A comprehensive analysis of the transcriptional and metabolic landscape of cervical squamous cell carcinoma (CSCC) at high resolution could greatly enhance the precision of management and therapeutic strategies for this malignancy.  \nMethods The Air-flow-assisted Desorption Electrospray Ionization Mass Spectro-metric Imaging (AFADESI-MSI) and Spatial Transcriptomics techniques (ST) were employed to investigate the metabolic and transcription profiles of CSCC and normal tissues. For clinical validation, the expression of ASCT2(Ala, Ser, Cys transporter 2) was assessed using immune histochemistry in 122 cases of cervical cancer and 30 cases of cervicitis.  \nResults The AFADESI-MSI findings have revealed metabolic differences among different CSCC patients. Among them, the metabolic pathways of glutamine show more significant differences. After in situ detection of metabolites, the intensity of glutamate is observed to be significantly higher in cancerous tissue compared to normal tissue, but the intensity is not uniform. To elucidate the potential factors underlying alterations in glutamine metabolism across tissues, we employ ST to quantify mRNA levels. This analysis unveils significant perturbations in glutamine metabolism accompanied by extensive heterogeneity within cervical cancer tissues. After conducting a comprehensive analysis, it has been revealed that the differential expression of ASCT2(encoded by SLC1A5) in distinct regions of cervical cancer tissues plays a pivotal role in inducing heterogeneity in glutamine metabolism. Furthermore, the higher the expression level of ASCT2, the higher the intensity of glutamate is in the region. Further verification, it is found that the expression of ASCT2 protein in CSCC tissues is significantly higher than that in normal tissues (105/122, 86 . 07%) .  \nConclusions This finding suggests that the variation in glutamine metabolism is not uniform throughout the tumor. The differential expression of ASCT2 in different regions of cervical cancer tissues seems to play a key role in causing this heterogeneity. This research has opened up new avenues for exploring the glutamine metabolic characteristics of CSCC which is essential for developing more effective targeted therapies.  \nKeywords Cervical carcinoma, Glutamine metabolism, Heterogeneity, AFADESI-MSI, ST  \n*Correspondence: Guzalinuer Abulizi[gzlnr@qq.com](gzlnr@qq.com)[ ](gzlnr@qq.com)Ayshamgul Hasim[axiangu@xjmu.edu.cn](axiangu@xjmu.edu.cn)  \nFull list of author information is available at the end of the article  \n© The Author(s) 2024. Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use,","cbCaitCuaM8GgfGC","https://ap.wps.com/l/cbCaitCuaM8GgfGC","pdf",5984729,13,"English","# Abstract\n## Background\n## Methods\n## Results\n## Conclusions\n# Background","[{\"question\":\"What methods were used to study glutamine metabolism in cervical cancer tissues?\",\"answer\":\"AFADESI-MSI was used for airflow-assisted desorption electrospray ionization mass spectrometry imaging, and spatial transcriptomics (ST) was used to quantify mRNA levels. Immune histochemistry was used for clinical validation of ASCT2 expression.\"},{\"question\":\"What did AFADESI-MSI reveal about glutamine metabolism in different patients and tissues?\",\"answer\":\"Metabolic differences were detected among cervical squamous cell carcinoma patients, with glutamine-related pathways showing stronger variation. Glutamate intensity was higher in cancerous tissue than normal tissue, and it varied across regions rather than being uniform.\"},{\"question\":\"How is ASCT2 related to metabolic heterogeneity in cervical carcinoma?\",\"answer\":\"Differential ASCT2 expression (encoded by SLC1A5) across tumor regions was identified as a key factor driving heterogeneity in glutamine metabolism. Higher ASCT2 expression corresponded to higher glutamate intensity in the same region, and ASCT2 protein levels were higher in CSCC tissues than in normal tissues.\"}]","Metabolism and spatial transcription resolved heterogeneity of glutamine metabolism in cervical carcinoma - Research paper | PDF",1789984634,33]