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Margin assessment relies on intraoperative pathological diagnosis, yet surgical margin designation is controversial. This study analyzes metabolomic profiles of tissues sampled at different incision-edge distances to detect metabolic differences and potential biomarkers. Mass spectrometry and KEGG pathway analysis identify significant metabolic changes mainly at the 1 mm margin, supporting its adequacy and informing margin selection.",{"@graph":69,"@context":126},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/metabolic-characteristics-of-the-various-incision-margins-for-breast-cancer-conservation-surgery-research-article/383213/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/metabolic-characteristics-of-the-various-incision-margins-for-breast-cancer-conservation-surgery-research-article/383213.png","ImageObject",300,407,{"name":92,"@type":93},"nayy☆","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-28","2026-09-24",true,{"@type":102,"interactionType":103,"userInteractionCount":52},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118,122],{"name":109,"@type":110,"acceptedAnswer":111},"Why are surgical margins in breast-conserving surgery controversial?","Question",{"text":112,"@type":113},"Different margin widths trade off between postoperative aesthetics and risks of reoperation and local recurrence, leading to controversy in how margins are designated.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How was the tissue sampling and grouping performed in this study?",{"text":117,"@type":113},"Samples were collected from 10 patients and surrounding tissues, then divided into cancerous tissue and cutting-edge tissues at 1 mm, 2 mm, 3 mm, 5 mm, and 10 mm, yielding 60 samples in total.",{"name":119,"@type":110,"acceptedAnswer":120},"Which incision margin showed the strongest metabolic distinction versus cancer tissue?",{"text":121,"@type":113},"Statistical analysis showed substantial differences between the 1 mm margin tissue and cancer tissue, while 1 mm did not differ significantly from other margins.",{"name":123,"@type":110,"acceptedAnswer":124},"What metabolites and pathways were identified as potential markers?",{"text":125,"@type":113},"Six metabolites—pyruvate, N-acetyl-L-aspartate, glutamic acid, gamma-aminobutyric acid, fumaric acid, and citric acid—were highlighted, and KEGG analysis indicated distinct amino-acid metabolism and tRNA synthesis-related pathways.","https://schema.org",{"og:url":83,"og:type":128,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":130,"canonical":83},"index,follow",{"doc_id":132,"site_id":62},383213,1790509991,{"code":4,"msg":5,"data":135},{"doc_id":132,"user_id":136,"nickname":92,"user_avatar":137,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":138,"file_id":139,"file_url":140,"file_type":141,"file_size":142,"view_count":52,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":143,"language":144,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":145,"faqs":146,"seo_title":147,"seo_description":67,"update_tm":148,"read_time":149},962090893153,"https://ap-avatar.wpscdn.com/davatar_9964176cb1d06d4a9deccf72a44ae3dc","TYPE Original Research PUBLISHED 04 January 2023 DOI 10.3389/fonc.2022.959454  \nOPEN ACCESS  \nEDITED BY  \nDayanidhi Raman,  \nUniversity of Toledo, United States  \nREVIEWED BY  \nAssie Olfatbakhsh,  \nMotamed Cancer Institute, Iran Teodora Alexa-Stratulat,  \nGrigore T. Popa, University of Medicine and Pharmacy, Romania  \n*CORRESPONDENCE  \nMingli Han  \n [Minglihan@126.com](Minglihan@126.com)  \n†These authors have contributed equally to this work and share first authorship  \nSPECIALTY SECTION  \nThis article was submitted to Breast Cancer,  \na section of the journal Frontiers in Oncology  \nRECEIVED 01 June 2022  \nACCEPTED 14 December 2022  \nPUBLISHED 04 January 2023  \nCITATION  \nWang F, Gu Z, Zhao X, Chen Z, Zhang Z, Sun S and Han M (2023) Metabolic characteristics of the various incision margins for breast cancer conservation surgery.  \nFront. Oncol. 12:959454 .  \ndoi: 10.3389/fonc.2022.959454  \nCOPYRIGHT  \n© 2023 Wang, Gu, Zhao, Chen, Zhang, Sun and Han. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) . The use, distribution or reproduction in other forums is permitted, provided the original author (s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.  \nMetabolic characteristics of the various incision margins for breast cancer conservation surgery  \nFang Wang †, Zongze Gu †, Xunan Zhao, Zhuo Chen, Zhe Zhang, Shihao Sun and Mingli Han*  \nDepartment of Breast Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China  \nBackground: Breast cancer (BC) has recently become the most prevalent malignancy in women. There are many alternative treatments for BC, and for aesthetic and postoperative quality of life concerns, breast-conserving surgery and corresponding adjuvant therapy have become the predominant treatment for early invasive BC. Currently, the main method used to assess the margins for breast-conserving surgery is intraoperative pathological diagnosis. However, the designation of surgical margins is controversial, and metabolomics may bea novel approach to evaluate surgical margins.  \nMethods: We collected specimens from 10 breast cancer patients and samples from its surrounding tissues and divided them into cancerous tissue and 1 mm, 2 mm, 3 mm, 5 mm and 10 mm cutting edge tissues, with a total of 60 samples. The samples were analyzed by mass spectrometry on an ultra-performance liquid chromatography-quadrupole/Orbitrap high resolution platform. The data were then statistically analyzed to detect metabolic changes in the different cutting edges and to identify possible surgical cutting edges with statistically significant findings. Abnormal metabolic pathways were identified by Kyoto Encyclopedia of Genes and Genomes (KEGG), which elucidated potential markers.  \nResults: Statistical analysis indicated that there were substantial differences between the 1 mm margin tissue and the cancer tissue, while there were no statistically significant differences between the 1 mm tissue and tissues from the other margins. The levels of 6 metabolites in the 1 mm tissue were significantly different from those in the cancer tissue and were not  \nsignificantly different from those in the 2 mm tissue. The six metabolites were pyruvate, N-acetyl-L-aspartate, glutamic acid, g-aminobutyric acid, fumaric acid, and citric acid. Metabolic pathways such as amino acid metabolism and a mino t-RNA synthesis in the margin tissue were significantly distinct from those in cancer tissues based on KEGG analysis.  \nFrontiers in Oncology 01 [frontiersin.org](frontiersin.org)  \nConclusion: There was a significant difference between the 1 mm margin tissue and the cancerous tissue. Based on metabolomic analysis, the 1 mm negative margin is sufficient for surgery, and the six metabolites that we identified as","cbCaiuRSt5zbgXoy","https://ap.wps.com/l/cbCaiuRSt5zbgXoy","pdf",4550960,11,"English","# Background\n# Methods\n# Results\n# Conclusion\n# Keywords\n# Introduction","[{\"question\":\"Why are surgical margins in breast-conserving surgery controversial?\",\"answer\":\"Different margin widths trade off between postoperative aesthetics and risks of reoperation and local recurrence, leading to controversy in how margins are designated.\"},{\"question\":\"How was the tissue sampling and grouping performed in this study?\",\"answer\":\"Samples were collected from 10 patients and surrounding tissues, then divided into cancerous tissue and cutting-edge tissues at 1 mm, 2 mm, 3 mm, 5 mm, and 10 mm, yielding 60 samples in total.\"},{\"question\":\"Which incision margin showed the strongest metabolic distinction versus cancer tissue?\",\"answer\":\"Statistical analysis showed substantial differences between the 1 mm margin tissue and cancer tissue, while 1 mm did not differ significantly from other margins.\"},{\"question\":\"What metabolites and pathways were identified as potential markers?\",\"answer\":\"Six metabolites—pyruvate, N-acetyl-L-aspartate, glutamic acid, gamma-aminobutyric acid, fumaric acid, and citric acid—were highlighted, and KEGG analysis indicated distinct amino-acid metabolism and tRNA synthesis-related pathways.\"}]","Metabolic characteristics of the various incision margins for breast cancer conservation surgery - Research article | PDF",1790255261,28]