[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-453066-105":59,"doc-detail-453066-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","membrane-repair-following-filtroporation-induced-cell-permeabilization","Membrane repair following filtroporation-induced cell permeabilization","","Mechanical disruption of cell membranes to deliver intracellular cargos is a growing approach. This study demonstrates that filtroporation (FP) enables efficient beta globin gene editing in human hematopoietic stem and progenitor cells. Transcriptome analyses from RNA-Seq show FP causes fewer global transcriptional changes than electroporation (EP), with pathway enrichment indicating EP favors stem cell differentiation whereas FP supports cell division. Membrane repair after FP occurs within 30 seconds, involves calcium signaling, and requires multiple repair mechanisms.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/membrane-repair-following-filtroporation-induced-cell-permeabilization/453066/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/membrane-repair-following-filtroporation-induced-cell-permeabilization/453066.png","ImageObject",300,407,{"name":92,"@type":93},"Finn","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-30","2026-09-30",true,{"@type":102,"interactionType":103,"userInteractionCount":29},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What is the main finding about membrane repair after filtroporation?","Question",{"text":112,"@type":113},"Cells repair their membranes within 30 seconds following filtroporation-induced disruption. The process involves calcium signaling and multiple repair mechanisms.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How does filtroporation compare with electroporation in transcriptional effects?",{"text":117,"@type":113},"RNA-Seq analyses indicate electroporation produces greater transcriptional changes globally than filtroporation.",{"name":119,"@type":110,"acceptedAnswer":120},"What does the study suggest about how the delivery method affects stem cell behavior?",{"text":121,"@type":113},"Gene pathway enrichment suggests electroporation promotes stem cell differentiation, while filtroporation promotes cell division, leading to different transcriptional stem cell profiles.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},453066,1790906865,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":29,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":56,"language":139,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":140,"faqs":141,"seo_title":142,"seo_description":67,"update_tm":143,"read_time":144,"preview_page_count":4},34359740700684,"https://ap-avatar.wpscdn.com/avatar/1f400023980c374ae676?_k=1777273430885731487","iScience Article  \nMembrane repair following filtroporation-induced cell permeabilization  \nAuthors  \nIsaura M. Frost, Ruby Sims,  \nAnya Yakimenko, ..., Emily Skuratovsky, Paul S. Weiss, Steven J. Jonas  \nCorrespondence  \n[psw@cnsi.ucla.edu](psw@cnsi.ucla.edu) (P.S.W.),  \n[sjonas@ucla.edu](sjonas@ucla.edu) (S.J.J.)  \nIn brief  \nCell biology; Omics  \n• Filtroporation causes fewer transcriptional changes than electroporation  \n• Cells repair their membranes within 30 s of filtroporation  \n• Multiple membrane repair mechanisms are involved following filtroporation  \nFrost et al., 2026, iScience 29, 114317  \nJanuary 16, 2026 © 2025 The Author(s) . Published by Elsevier Inc.  \n[https://doi.org/10.1016/j.isci.2025.114317](https://doi.org/10.1016/j.isci.2025.114317)  \nll  \niScience  \nll  \nOPEN ACCESS  \nArticle  \nMembrane repair following  \nfiltroporation-induced cell permeabilization  \nIsaura M. Frost,1,2,3 Ruby Sims,3 Anya Yakimenko,3 Rachel Ma,3,4 Maximilian Floridia,4 Ruth A. Foley,1,3 Emily C. Duggan,3 Kelly Y. Cai,5 Kelsey Jorgensen,6 Emily Skuratovsky,7 Paul S. Weiss,1,4,8,9,* and Steven J. Jonas3,7,10,11,*  \n1Department of Bioengineering, University of California, Los Angeles, Los Angeles, CA 90095, USA  \n2UCLA Medical Scientist Training Program, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA  \n3Department of Pediatrics, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA  \n4Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, CA 90095, USA  \n5Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095, USA  \n6Department of Anthropology, University of Kansas, Lawrence, KS 66045, USA  \n7Eli & Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, Los Angeles, CA 90095, USA  \n8California NanoSystems Institute, University of California, Los Angeles, Los Angeles, CA 90095, USA  \n9Department of Materials Science and Engineering, University of California, Los Angeles, Los Angeles, CA 90095, USA  \n10Children’s Discovery and Innovation Institute, University of California, Los Angeles, Los Angeles, CA 90095, USA  \n11Lead contact  \n*Correspondence: [psw@cnsi.ucla.edu](psw@cnsi.ucla.edu) (P.S.W.), [sjonas@ucla.edu](sjonas@ucla.edu) (S.J.J.) [https://doi.org/10.1016/j.isci.2025.114317](https://doi.org/10.1016/j.isci.2025.114317)  \nSUMMARY  \nMechanical disruption of cell membranes to enable intracellular delivery has been gaining traction as a methodology. Here, we show that a mechanical disruption-based strategy, filtroporation (FP), can be applied to edit the beta globin gene efficiently in human hematopoietic stem and progenitor cells. Gene expression analyses from RNA-Seq datasets demonstrate that electroporation (EP) yields greater transcriptional changes compared to FP globally, and gene pathway enrichment analyses suggest EP promotes stem cell differentiation while FP promotes cell division. Membrane repair occurs within 30 s of disruption by FP, and calcium signaling plays a key role in membrane repair in this context. Studies with fluorescently tagged membrane repair proteins, GRAF1, SNAP23, and CHMP4B, implicate the involvement of three mechanisms to reconstitute the cell barrier following FP. This work supports the evidence that the choice of intracellular delivery method affects transcriptional stem cell profile, and that membrane repair after mechanical disruption is a fast, multi-pathway process.  \nINTRODUCTION  \nAchieving effective intracellular delivery of biomolecular cargoes by the transient permeabilization of cellular membrane barriers represents an ongoing challenge in both research and clinical settings.1–3 Several delivery methodologies are under development that may enable more efficient manufacturing of next-generation cell-based therapies and do not require the use of","cbCaiuQZ5yjUz8qc","https://ap.wps.com/l/cbCaiuQZ5yjUz8qc","pdf",6538728,"English","# Summary\n## Membrane repair timing and mechanisms\n# Introduction\n## Challenges in intracellular delivery without viral vectors\n## Mechanical deformation and transient pores\n## Limitations of equipment-dependent approaches\n# Summary of study rationale and prior work","[{\"question\":\"What is the main finding about membrane repair after filtroporation?\",\"answer\":\"Cells repair their membranes within 30 seconds following filtroporation-induced disruption. The process involves calcium signaling and multiple repair mechanisms.\"},{\"question\":\"How does filtroporation compare with electroporation in transcriptional effects?\",\"answer\":\"RNA-Seq analyses indicate electroporation produces greater transcriptional changes globally than filtroporation.\"},{\"question\":\"What does the study suggest about how the delivery method affects stem cell behavior?\",\"answer\":\"Gene pathway enrichment suggests electroporation promotes stem cell differentiation, while filtroporation promotes cell division, leading to different transcriptional stem cell profiles.\"}]","Membrane repair following filtroporation-induced cell permeabilization | PDF",1790738174,48]