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PDLF senescence was assessed using β-galactosidase staining, Western blotting, and immunofluorescence, with functional readouts including Alizarin Red S mineralization and TRAP-based osteoclast differentiation. YAP signaling was analyzed by Western blot and immunofluorescence, including pharmacological modulation with XMU-MP-1 and pathway agonists/inhibitors. Results show compressive forces inactivate mechanosensitive YAP, upregulate cGAS/STING signaling, and impair mineralization, with STING activation exacerbating effects.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/mechanosensitive-yes-associated-proteintaz-cgas-sting-axis-induces-periodontal-ligament-fibroblasts-senescence-and-mediates-compression-induced-root-resorption/415621/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/mechanosensitive-yes-associated-proteintaz-cgas-sting-axis-induces-periodontal-ligament-fibroblasts-senescence-and-mediates-compression-induced-root-resorption/415621.png","ImageObject",300,407,{"name":92,"@type":93},"Sarah ","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-30","2026-09-28",true,{"@type":102,"interactionType":103,"userInteractionCount":44},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What is the main objective of the study?","Question",{"text":112,"@type":113},"To determine whether compressive forces induce senescence in periodontal ligament fibroblasts and to clarify how this senescence mechanistically drives orthodontic root resorption.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How was PDLF senescence evaluated under compressive forces?",{"text":117,"@type":113},"Senescence was assessed using β-galactosidase staining, Western blotting, and immunofluorescence.",{"name":119,"@type":110,"acceptedAnswer":120},"What mechanistic pathway links YAP to cGAS/STING in compression-induced effects?",{"text":121,"@type":113},"Compressive forces inactivate mechanosensitive YAP, which upregulates cGAS/STING signaling; activating STING worsens senescence and impairs mineralization, while YAP activation reduces senescence and osteoclastogenesis and STING inhibition reverses these effects.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},415621,1790910789,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":44,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145,"preview_page_count":4},962085320529,"https://ap-avatar.wpscdn.com/davatar_9964176cb1d06d4a9deccf72a44ae3dc","Scientific Research Report  \nMechanosensitive Yes-Associated Protein/TAZ−cGAS −STING Axis Induces Periodontal Ligament  \nFibroblasts Senescence and Mediates Compression-Induced Root Resorption  \nZuping Wu a,b,y, Siqi Bao a,y, Qian Chena, Xiao Luc, Guzhe Guana, Ruifeng Song a, Qianming Chena*, Xiaoyan Chen a*  \na Stomatology Hospital, School of Stomatology, Zhejiang University School of Medicine, Zhejiang Provincial Clinical Research Center for Oral Diseases, Key Laboratory of Oral Biomedical Research of Zhejiang Province, Cancer Center of Zhejiang University, Engineering Research Center of Oral Biomaterials and Devices of Zhejiang Province, Hangzhou, China  \nb Guangxi Key Laboratory of the Rehabilitation and Reconstruction for Oral and Maxillofacial Research, Affiliated Hospital of Stomatology, Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region, China  \nc Case Western Reserve University Dental School, Cleveland, Ohio, USA  \n| A R T I C L E I N F O | A B S T R A C T |\n| --- | --- |\n| Article history:\u003Cbr>Received 1 September 2025\u003Cbr>Received in revised form\u003Cbr>31 October 2025\u003Cbr>Accepted 5 November 2025\u003Cbr>Available online 11 December 2025 | Objective: This study investigates compressive force-induced senescence in periodontal ligament fibroblasts (PDLFs) and its mechanistic role in orthodontic root resorption.\u003Cbr>Materials and Methods: PDLFs senescence under compressive forces was evaluated using b-galactosidase staining, Western blotting, and immunofluorescence. Functional consequences of senescence were assessed via Alizarin Red S for mineralization and TRAP staining for osteoclast differentiation. Yes-associated protein (YAP) signalling dynamics were analysed through Western blot and immunofluorescence, with pharmacological activation by XMU-MP-1 . The interplay between YAP and the cGAS/STING pathway was investigated using pharmacological interventions. Finally, senescence-mediated effects on mineralization and osteoclastogenesis were evaluated post-treatment with pathway-specific agonistsor inhibitors.\u003Cbr>Results: Compressive forces induced senescence in PDLFs, evidenced by SA-b-gal activation and reduced mineralization capacity. Coculture with osteoclast precursors amplified osteoclast differentiation. Mechanistically, compressive forces inactivated mechanosensitive YAP, triggering senescence, rescued by YAP agonist XMU-MP-1 . The YAP/cGAS/STING axis was identified as central: YAP inactivation upregulated cGAS/STING signalling, while STING agonist c-di-GMP exacerbated senescence and impaired mineralization. Pharmacological validation confirmed bidirectional regulation − YAP activation reduced senescence/ osteoclastogenesis, whereas STING inhibition reversed these effects.\u003Cbr>Conclusion: Mechanical stress-induced YAP dysfunction drives PDLF senescence via cGAS/ STING pathway activation, establishing a novel mechanobiological axis in root resorption.\u003Cbr>􀀁 2025 The Authors. Published by Elsevier Inc. on behalf ofFDI World Dental Federation. This is an open access article under the CC BY-NC-ND license\u003Cbr>([http://creativecommons.org/licenses/by-nc-nd/4.0/](http://creativecommons.org/licenses/by-nc-nd/4.0/)) |\n| Key words:\u003Cbr>Root resorption\u003Cbr>YAP\u003Cbr>cGAS/STING\u003Cbr>Periodontal ligament fibroblasts Senescence |  |\n\n* Corresponding author. Stomatology Hospital, School of Stomatology, Zhejiang University School of Medicine, Zhejiang Provincial Clinical Research Center for Oral Diseases, Key Laboratory of Oral Biomedical Research of Zhejiang Province, Cancer Center of Zhejiang University, Engineering Research Center of Oral Biomaterials and Devices of Zhejiang Province, Hangzhou 310000, China.  \n[E-mail addresses:](E-mail addresses: qmchen@zju.edu.cn)[ qmchen@zju.edu.cn](E-mail addresses: qmchen@zju.edu.cn) (Q. Chen), [ortho_chenxy@zju.edu.cn](ortho_chenxy@zju.edu.cn) (X. Chen).  \nZuping Wu: [http://orcid.org/0000-0003-3661-8593](http://orcid.org/0000-0003-3661-8593)  \ny Zuping Wu and Siqi Bao contributed equally to this w","cbCaibABkSxAIhXR","https://ap.wps.com/l/cbCaibABkSxAIhXR","pdf",6150065,14,"English","# Article history\n## Introduction\n## Materials and Methods\n## Results\n## Conclusion","[{\"question\":\"What is the main objective of the study?\",\"answer\":\"To determine whether compressive forces induce senescence in periodontal ligament fibroblasts and to clarify how this senescence mechanistically drives orthodontic root resorption.\"},{\"question\":\"How was PDLF senescence evaluated under compressive forces?\",\"answer\":\"Senescence was assessed using β-galactosidase staining, Western blotting, and immunofluorescence.\"},{\"question\":\"What mechanistic pathway links YAP to cGAS/STING in compression-induced effects?\",\"answer\":\"Compressive forces inactivate mechanosensitive YAP, which upregulates cGAS/STING signaling; activating STING worsens senescence and impairs mineralization, while YAP activation reduces senescence and osteoclastogenesis and STING inhibition reverses these effects.\"}]","Mechanosensitive Yes-Associated Protein/TAZ-cGAS-STING Axis Induces Periodontal Ligament Fibroblasts Senescence and Mediates Compression-Induced Root Resorption | PDF",1790602176,35]