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METHODS: Eighty-seven participants were allocated to mnLC (n=30), benign lung nodule (n=27), and healthy control (n=30) groups; sEV morphology and markers were evaluated by TEM, NTA, and Western blot, and functional effects were tested using miR-412-3p/TEAD1 signaling assays plus serum sEV validation.",{"@graph":69,"@context":123},[70,84,106],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/mechanism-study-of-serum-extracellular-nano-vesicles-mir-412-3p-targeting-regulation-of-tead1-in-promoting-malignant-biological-behavior-of-sub-centimeter-lung-nodules/331436/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/mechanism-study-of-serum-extracellular-nano-vesicles-mir-412-3p-targeting-regulation-of-tead1-in-promoting-malignant-biological-behavior-of-sub-centimeter-lung-nodules/331436.png","ImageObject",300,407,{"name":92,"@type":93},"River Wang","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-29","2026-09-21",true,{"@type":102,"interactionType":103,"userInteractionCount":105},"InteractionCounter",{"@type":104},"ViewAction",12,{"@type":107,"mainEntity":108},"FAQPage",[109,115,119],{"name":110,"@type":111,"acceptedAnswer":112},"What is the study objective regarding miR-412-3p and sEVs in lung nodules?","Question",{"text":113,"@type":114},"To investigate how serum extracellular nano-vesicles carrying miR-412-3p, released from 6–10 mm sub-centimeter lung nodules, affect malignant biological functions of micro-nodular lung cancer.","Answer",{"name":116,"@type":111,"acceptedAnswer":117},"How were sEVs characterized and participant groups organized?",{"text":118,"@type":114},"Eighty-seven participants were divided into mnLC, benign lung nodule, and healthy control groups, and sEV morphology and surface markers were measured using TEM, NTA, and Western blot.",{"name":120,"@type":111,"acceptedAnswer":121},"What role does the miR-412-3p/TEAD1 signaling axis play in lung cancer cells?",{"text":122,"@type":114},"miR-412-3p negatively regulates TEAD1 and the miR-412-3p/TEAD1 axis promotes EMT-related signaling, enhancing proliferation, migration, and stemness while affecting E-cadherin, N-cadherin, and Vimentin expression.","https://schema.org",{"og:url":83,"og:type":125,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":127,"canonical":83},"index,follow",{"doc_id":129,"site_id":62},331436,1790014833,{"code":4,"msg":5,"data":132},{"doc_id":129,"user_id":133,"nickname":92,"user_avatar":134,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":135,"file_id":136,"file_url":137,"file_type":138,"file_size":139,"view_count":105,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":140,"language":141,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":142,"faqs":143,"seo_title":144,"seo_description":67,"update_tm":145,"read_time":146},1099514067438,"https://ap-avatar.wpscdn.com/avatar/100002539ee87300030?x-image-process=image/resize,m_fixed,w_180,h_180&k=1780474512215547542","Cancer Biomarkers 41 (2024) 69–82 69  \nDOI 10.3233/CBM-240137 IOS Press  \nMechanism study of serum extracellular nano-vesicles miR-412-3p targeting regulation of TEAD1 in promoting malignant biological behavior of sub-centimeter lung nodules  \nYuxia Denga ; 1 , Nishant Patelb ; 1 , Shuang Dingc and Haijun Zhanga ;􀀃  \na Department of Oncology, Zhongda Hospital, Medical School, Southeast University, Nanjing, China b Department of Cardiothoracic Surgery, Children's Hospital of Nanjing Medical University, Nanjing, China c Department of Oncology, Nanjing First Hospital, Nanjing Medical University, Nanjing, China  \nReceived 13 May 2024 Accepted 31 July 2024  \nAbstract.  \nOBJECTIVE: To investigate the impact and potential mechanisms of serum extracellular nano-vesicles (sEVs) miR-412-3p released from sub-centimeter lung nodules with a diameter of 6 10 mm on the malignant biological function of micro-nodular lung cancer (mnLC) .  \nMETHODS: A total of 87 participants were included and divided into a mnLC group (n = 30), a benign lung nodule (BLN) group (n = 27), and a healthy people control group (n = 30). Transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA) and Western blot (WB) were used to measure the morphological characteristics and surface markers of sEVs. In vitro analysis, real-time quantitative polymerase chain reaction (RT-qPCR), CCK-8 cell proliferation assay, clone formation assay, Transwell, stem cell sphere-forming assay, and WB assay were conducted to verify the effect of miR-412-3p/TEAD1 signaling axis on the biological function of lung cancer cells through, respectively. Further validation was conducted using the serum sEVs of the participants.  \nRESULTS: The expression level of sEVs-miR-412-3p in the mnLC group was significantly higher than that in the BLN and healthy groups (P \u003C 0.01) . In lung cancer cell lines, miR-412-3p can negatively regulate the targeted gene TEAD1 . The miR-412- 3p/TEAD1 signaling axis is involved in promoting the EMT signaling pathway and regulating the malignant biological functions of lung cancer cell proliferation, migration, and stemness (P \u003C 0.05) . In addition, sEVs in the mnLC group significantly promoted lung cancer cell proliferation, migration, and stemness compared to the BLN and healthy groups, inhibited the expression of E-cadherin and TEAD1 in lung cancer cells, and promoted the expression of N-cadherin and Vimentin (P \u003C 0.05) . CONCLUSION: sEVs-miR-412-3p could promote the biological process of EMT, and lead to the occurrence of malignant biological behavior in sub-centimeter lung nodules. This provides evidence for the miR-412-3p/TEAD1 signaling axis as a potential therapeutic target for mnLC.  \nKeywords: Micro-nodular lung cancer, serum extracellular nano-vesicles, miR-412-3p, transcriptional enhancer associated domain 1, epithelial mesenchymal transition  \n1These authors contributed equally.  \n􀀃 Corresponding author: Haijun Zhang, Department of Oncology, Zhongda Hospital, Medical School, Southeast University, Nanjing,  \n210009, [China. E-mail: haijunzhang@seu.edu.cn](China. E-mail: haijunzhang@seu.edu.cn), zhanghaijunseu@  \n[163.com](163.com).  \nISSN 1574-0153 􀀍c 2024 – The authors. Published by IOS Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (CC BY-NC 4 .0) .  \n70 Y. Deng et al. / Mechanism study of serum extracellular nano-vesicles miR-412-3p targeting regulation of TEAD1  \n1. Introduction  \nLung cancer is one of the most common and poorly prognostic cancers worldwide, and also one of the most burdensome malignant tumors worldwide, seriously endangering human health [1,2] . Therefore, the early diagnosis and treatment of lung cancer are of great significance for improving the quality of life of patients and reducing mortality [3,4] . With the widespread application of low-dose spiral computed tomography (LDCT) in clinical practice, the detection rate of sub-centimeter lung","cbCaiaHKyQlp9e6C","https://ap.wps.com/l/cbCaiaHKyQlp9e6C","pdf",2676824,14,"English","# Abstract\n# Introduction","[{\"question\":\"What is the study objective regarding miR-412-3p and sEVs in lung nodules?\",\"answer\":\"To investigate how serum extracellular nano-vesicles carrying miR-412-3p, released from 6–10 mm sub-centimeter lung nodules, affect malignant biological functions of micro-nodular lung cancer.\"},{\"question\":\"How were sEVs characterized and participant groups organized?\",\"answer\":\"Eighty-seven participants were divided into mnLC, benign lung nodule, and healthy control groups, and sEV morphology and surface markers were measured using TEM, NTA, and Western blot.\"},{\"question\":\"What role does the miR-412-3p/TEAD1 signaling axis play in lung cancer cells?\",\"answer\":\"miR-412-3p negatively regulates TEAD1 and the miR-412-3p/TEAD1 axis promotes EMT-related signaling, enhancing proliferation, migration, and stemness while affecting E-cadherin, N-cadherin, and Vimentin expression.\"}]","Mechanism study of serum extracellular nano-vesicles miR-412-3p targeting regulation of TEAD1 in promoting malignant biological behavior of sub-centimeter lung nodules | PDF",1789993256,35]