[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-331819-105":59,"doc-detail-331819-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","mdm4-was-associated-with-poor-prognosis-and-tumor-immune-infiltration-of-cancers","MDM4 was associated with poor prognosis and tumor-immune infiltration of cancers","","MDM4 is a negative regulator of p53 and a cancer-promoting factor involved in tumorigenesis and progression. This study analyzed MDM4 expression across pan-cancer datasets and evaluated its associations with prognosis, immune-related features, genetic mutations, and tumor-associated pathways. MDM4 overexpression correlated with adverse clinical characteristics, poor outcomes, oncogenic mutations, tumor-immune infiltration patterns, and dysregulated oncogenic signaling. Transcriptomic sequencing and qPCR were used in colon cancer, while in vitro assays and an MDM4 inhibitor (NSC146109) assessed anti-tumor effects.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/mdm4-was-associated-with-poor-prognosis-and-tumor-immune-infiltration-of-cancers/331819/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/mdm4-was-associated-with-poor-prognosis-and-tumor-immune-infiltration-of-cancers/331819.png","ImageObject",300,407,{"name":92,"@type":93},"Logic","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-28","2026-09-21",true,{"@type":102,"interactionType":103,"userInteractionCount":34},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What role does MDM4 play in cancers according to the study?","Question",{"text":112,"@type":113},"MDM4 functions as a negative regulator of p53 and acts as a cancer-promoting factor involved in tumor development and progression.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How did the researchers evaluate the clinical and biological significance of MDM4?",{"text":117,"@type":113},"They analyzed MDM4 expression across pan-cancer databases and examined correlations with prognosis, immune features, genetic mutations, and tumor-related pathways.",{"name":119,"@type":110,"acceptedAnswer":120},"What experimental approaches were used to test MDM4-related anti-tumor effects?",{"text":121,"@type":113},"The study used transcriptomic sequencing and qPCR in colon cancer, and performed in vitro assays such as colony formation, chemoresistance, and senescence-associated β-galactosidase activity with the MDM4 inhibitor NSC146109.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},331819,1791074756,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":34,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145,"preview_page_count":4},1099513958762,"https://ap-avatar.wpscdn.com/avatar/1000023916a998db790?x-image-process=image/resize,m_fixed,w_180,h_180&k=1784791008015729253","Liu et al. European Journal  \nEuropean Journal of Medical Research (2024) 29:79  \n[https://doi.org/10.1186/s40001-024-01684-z](https://doi.org/10.1186/s40001-024-01684-z of Medical Research)[ of Medical Research](https://doi.org/10.1186/s40001-024-01684-z of Medical Research)  \n RESEARCH Open Access  \nMDM4 was associated with poor prognosis   and tumor-immune infiltration of cancers  \nJie Liu1,2,3†, Jie Yang2,3†, Qilong Pan1, Xiangyu Wang1,2,3, Xinyin Wang1, Han Chen1, Xiaoling Zheng2,3* and Qingling Huang1*  \nAbstract  \nMDM4 is one of the MDM protein family and is generally recognized as the key negative regulator of p53 . As a cancer-promoting factor, it plays a non-negligible role in tumorigenesis and development. In this article, we analyzed the expression levels of MDM4 in pan-cancer through multiple databases. We also investigated the correlations between MDM4 expression and prognostic value, immune features, genetic mutation, and tumor-related pathways. We found that MDM4 overexpression is often accompanied by adverse clinical features, poor prognosis, oncogenic mutations, tumor-immune infiltration and aberrant activation of oncogenic signaling pathways. We also conducted transcriptomic sequencing to investigate the effect of MDM4 on transcript levels in colon cancer and performed qPCR to verify this. Finally, we carried out some in vitro experiments including colony formation assay, chemoresistance and senescence-associated β-galactosidase activity assay to study the anti-tumor treatment effect of small molecule MDM4 inhibitor, NSC146109 . Our research confirmed that MDM4 is a prognostic biomarker and potential therapeutic target for a variety of malignancies.  \nKeywords MDM4, Pan-cancer, Prognostic biomarker, Tumor-immune infiltration, Inhibitor  \nIntroduction  \nCancer is the leading cause of death throughout the world according to estimations from the World Health Organization (WHO), which imposes a substantial health and economic burden because of its high mortality [1]. Traditional treatments like surgery, chemotherapy or radiation therapy, prolong survival in patients with tumors, and encounter stringent bottlenecks simultaneously.  \n†Jie Liu and Jie Yang contributed equally to this work.  \n*Correspondence:  \nXiaoling Zheng [xiaolingzheng@fjmu.edu.cn](xiaolingzheng@fjmu.edu.cn)[ ](xiaolingzheng@fjmu.edu.cn)Qingling Huang [QinglingHuang@fjmu.edu.cn](QinglingHuang@fjmu.edu.cn)  \n1 Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, China  \n2 Department of Endoscopy, Shengli Clinical Medical College of Fujian Medical University, Fujian Provincial Hospital, Fuzhou, China  \n3 The Graduate School of Fujian Medical University, Fuzhou, China  \nFor several years now, molecular-targeted therapy and immunotherapy have become promising approaches to own higher response rates and better prognosis in malignancies treatment. Discovering novel tumor biomarkers for early detection and widening the potential therapy  \noptions have substantial societal and medical impacts.  \nThe MDM gene family, called mouse double minute because of the isolation from small extrachromosomal bodies present in the mouse cell line, comprises MDM2 and MDM4 [2] . MDM2, first identified in 1992, has been elucidated to stimulate tumors’ development in multiple cancers through activating the ubiquitination of p53 mediated by the proteasome. Mouse Double Minute 4 (MDM4), also known as MDMX, has been mapped to chromosomal 1q32, the encoded protein of which consists of 490 amino acids. As oneof MDM family members, MDM4 has similar activities to MDM2 and also plays a negative regulatory role in p53 levels [3] . The N-terminal of MDM4 is a  \n© The Author(s) 2024. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to","cbCaitQU9BeE1YNi","https://ap.wps.com/l/cbCaitQU9BeE1YNi","pdf",5922088,17,"English","# Abstract\n# Keywords\n# Introduction","[{\"question\":\"What role does MDM4 play in cancers according to the study?\",\"answer\":\"MDM4 functions as a negative regulator of p53 and acts as a cancer-promoting factor involved in tumor development and progression.\"},{\"question\":\"How did the researchers evaluate the clinical and biological significance of MDM4?\",\"answer\":\"They analyzed MDM4 expression across pan-cancer databases and examined correlations with prognosis, immune features, genetic mutations, and tumor-related pathways.\"},{\"question\":\"What experimental approaches were used to test MDM4-related anti-tumor effects?\",\"answer\":\"The study used transcriptomic sequencing and qPCR in colon cancer, and performed in vitro assays such as colony formation, chemoresistance, and senescence-associated β-galactosidase activity with the MDM4 inhibitor NSC146109.\"}]","MDM4 was associated with poor prognosis and tumor-immune infiltration of cancers | PDF",1789995334,43]