[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"doc-detail-216871-en":3,"doc-seo-216871-105":30,"detail-sidebar-cat-0-en-105":91},{"code":4,"msg":5,"data":6},0,"success",{"doc_id":7,"user_id":8,"nickname":9,"user_avatar":10,"doc_module":4,"category_id":11,"category_name":12,"doc_title":13,"doc_description":14,"doc_content":15,"file_id":16,"file_url":17,"file_type":18,"file_size":19,"view_count":20,"is_deleted":4,"is_public":20,"is_downloadable":20,"audit_status":20,"page_count":21,"language":22,"language_code":23,"site_id":24,"html_lang":23,"table_of_contents":25,"faqs":26,"seo_title":27,"seo_description":14,"update_tm":28,"read_time":29},216871,2336477974920,"Mimi","https://ap-avatar.wpscdn.com/davatar_155a257f0dc6eb9ab79c44ca47cae57d",8,"Research & Report","Marilda Keico Taciro - Metabolic Engineering Applied to Biopolymer Production","Metabolic engineering approaches are applied to produce biodegradable polymers of the polyhydroxyalkanoates (PHA) family, focusing on medium-chain-length copolymers P(3HB-co-HAmcl). Steady-state bioreactor data are used for metabolic flux analysis (MFA) via freely available software, while continuous-mode bacterial cultures with multiple nutrient limitations generate additional information for MFA. Metabolic models are proposed, simulated, and validated, guiding genetic modifications and process strategies to enhance yields and productivities, with expansion to other bioproducts through a dedicated metabolic engineering group.","FAPESP BIOENERGY PROGRAM   \n[http://bioenfapesp.org](http://bioenfapesp.org)  \n engineering   \nMETABOLIC ENGINEERING APPLIED  \nTO BIOPOLYMER PRODUCTION  \nMarilda Keico Taciro  \nInstitute of Biomedical Sciences / University of São Paulo (ICB/USP)  \nFAPESP Process 2010/51692-1 | Term: Mar 2011 to Feb 2016 | Young Investigator  \nMetabolic flux analysis (MFA) and other tools of metabolic and biochemical engineering are currently being applied for the improvement of the production of biodegradable polymers belonging to the polyhydroxyalkanoates family (PHA), particularly poly-3-hydroxybutyrate-co-3-hydroxyalkanoate of mediumchain-length [P(3HB-co-HAmcl)]. Using steady-state data generate in bioreactor experiments, flux analysis is performed through a freely available software. Bacterial cultures in a continuous mode operation, employing the technique of multiple nutrient limitation to induce polymer accumulation is another strategy to study the role of such nutrients and to generate data to MFA. Metabolic models will be proposed, simulated and validated utilizing the experimental data from bioreactor cultivations. The analysis of the metabolic models will provide information to propose genetic modifications and process strategies, to obtain higher yields and productivities. It is expected that, by the end of this project, the metabolic engineering approach emphasizing the integration of different methodologies and procedures has been established. As a result, a Metabolic Engineering Group is being created in the Microbiology Department at ICB USP, in such a way that, processes involving other bioproducts could be approached in a similar manner.  \nSUMMARY OF RESULTS  \nTO DATE AND PERSPECTIVES  \nAnalysis of the tracing pattern on C-labelled PHA has been performed, evidencing for the first time the extreme effectiveness of this approach to determine fluxes distribution in the central metabolism involved on PHA production. Associated with elementary mode analysis of the metabolism, this procedure allowed the identification of biochemical engineering and/or genetic approaches to improve production of these polymers and to characterize bacterial strains for the production of other compounds. Different strains were characterized with respect to  \nPHA production. Those genes that showed promising performance and interest have been cloned. Thereby strains producing copolymers of P(3HB-co-HAmcl) in a controlled manner were successfully obtained. This group of copolymers has properties that have attracted great interest as they permit to obtain plastic films.  \nBioreactor experiments were done to validate metabolic models and to propose possible strategies to improve polymer process production. Chemostat cultures exploring the regions of multiple nutrient limitations were done. The resulting steady states enable the study of different physiological situations and result on a snapshot representing each situation.  \nThe genome sequencing performed in the studied bacteria (Pseudomonas LFM 046) enabled the construction of the model in genomic scale improving the metabolic modeling studies.  \nFigure 2. Minibioreactor (100 ml working volume) utilized on experiments with labeled carbon source. Illustration of how measurement of the 13C enrichment patterns can be used to identify active pathways. EMP (Embden-Meyerhof-Parnas), ED (Entner-Doudoroff), PP (Pentose phosphate)  \nMAIN PUBLICATIONS  \nCardinale-Rezende J, Alexandrino PMR, Nahat RATPS, Sant’Ana DPV, Silva LF, Gomez JGC, Taciro MK. 2015. Draft genome sequence of Pseudomonas sp. LFM046, a producer of medium-chain-length polyhydroxyalkanoate. Genome Announcements. 3(4): e00966-15 .  \nAlexandrino PMR, MendonçaTT, Guamán LPB, Cherix J, Lozano-Sakalauskas GC, Fujita A, Ramos Filho E, Long P, Padilla G, Taciro MK, Gomez JGC, Silva LF. 2015. Draft genome sequence of the polyhydroxyalkanoate-producing bacterium Burkholderia sacchari LMG 19450 isolated from Brazilian Sugarcane Plantation Soil. Genome An","cbCaivVrRYOeBQWu","https://ap.wps.com/l/cbCaivVrRYOeBQWu","pdf",92619,1,2,"English","en",105,"# Summary of Results\n## To Date and Perspectives\n## Main Publications","[{\"question\":\"What biopolymer production target does the project focus on?\",\"answer\":\"The work targets biodegradable polymers from the polyhydroxyalkanoates (PHA) family, particularly medium-chain-length poly-3-hydroxybutyrate-co-3-hydroxyalkanoate (P(3HB-co-HAmcl)).\"},{\"question\":\"How is metabolic flux analysis (MFA) performed in the study?\",\"answer\":\"Steady-state data from bioreactor experiments are analyzed using flux analysis performed through a freely available software. Continuous-mode cultures using multiple nutrient limitations also generate data to support MFA.\"},{\"question\":\"How have the researchers validated and improved their metabolic understanding?\",\"answer\":\"Genome sequencing enables construction of a genomic-scale model, and bioreactor experiments (including chemostat cultures under multiple nutrient limitations) validate metabolic models and support proposed strategies for improved production.\"}]","Marilda Keico Taciro - Metabolic Engineering Applied to Biopolymer Production | PDF",1788828055,5,{"code":4,"msg":31,"data":32},"ok",{"site_id":24,"language":23,"slug":33,"title":13,"keywords":34,"description":14,"schema_data":35,"social_meta":86,"head_meta":88,"extra_data":90,"updated_unix":28},"marilda-keico-taciro-metabolic-engineering-applied-to-biopolymer-production","",{"@graph":36,"@context":85},[37,53,68],{"@type":38,"itemListElement":39},"BreadcrumbList",[40,44,47,50],{"item":41,"name":42,"@type":43,"position":20},"https://docshare.wps.com","Home","ListItem",{"item":45,"name":46,"@type":43,"position":21},"https://docshare.wps.com/document/","Document",{"item":48,"name":12,"@type":43,"position":49},"https://docshare.wps.com/document/research-report/",3,{"item":51,"name":13,"@type":43,"position":52},"https://docshare.wps.com/document/marilda-keico-taciro-metabolic-engineering-applied-to-biopolymer-production/216871/",4,{"url":51,"name":13,"@type":54,"author":55,"headline":13,"publisher":57,"fileFormat":60,"inLanguage":23,"description":14,"dateModified":61,"datePublished":62,"encodingFormat":60,"isAccessibleForFree":63,"interactionStatistic":64},"DigitalDocument",{"name":9,"@type":56},"Person",{"url":41,"name":58,"@type":59},"DocShare","Organization","application/pdf","2026-09-11","2026-09-08",true,{"@type":65,"interactionType":66,"userInteractionCount":20},"InteractionCounter",{"@type":67},"ViewAction",{"@type":69,"mainEntity":70},"FAQPage",[71,77,81],{"name":72,"@type":73,"acceptedAnswer":74},"What biopolymer production target does the project focus on?","Question",{"text":75,"@type":76},"The work targets biodegradable polymers from the polyhydroxyalkanoates (PHA) family, particularly medium-chain-length poly-3-hydroxybutyrate-co-3-hydroxyalkanoate (P(3HB-co-HAmcl)).","Answer",{"name":78,"@type":73,"acceptedAnswer":79},"How is metabolic flux analysis (MFA) performed in the study?",{"text":80,"@type":76},"Steady-state data from bioreactor experiments are analyzed using flux analysis performed through a freely available software. Continuous-mode cultures using multiple nutrient limitations also generate data to support MFA.",{"name":82,"@type":73,"acceptedAnswer":83},"How have the researchers validated and improved their metabolic understanding?",{"text":84,"@type":76},"Genome sequencing enables construction of a genomic-scale model, and bioreactor experiments (including chemostat cultures under multiple nutrient limitations) validate metabolic models and support proposed strategies for improved production.","https://schema.org",{"og:url":51,"og:type":87,"og:title":13,"og:site_name":58,"og:description":14},"article",{"robots":89,"canonical":51},"index,follow",{"doc_id":7,"site_id":24},{"code":4,"msg":5,"data":92},[93,97,101,105,109,114,119,122,127,130,134],{"id":20,"doc_module":4,"doc_module_name":46,"category_name":94,"show_sort_weight":95,"slug":96},"Story & Novel",90,"story-novel",{"id":21,"doc_module":4,"doc_module_name":46,"category_name":98,"show_sort_weight":99,"slug":100},"Literature",80,"literature",{"id":52,"doc_module":4,"doc_module_name":46,"category_name":102,"show_sort_weight":103,"slug":104},"Exam",70,"exam",{"id":29,"doc_module":4,"doc_module_name":46,"category_name":106,"show_sort_weight":107,"slug":108},"Comic",60,"comic",{"id":110,"doc_module":4,"doc_module_name":46,"category_name":111,"show_sort_weight":112,"slug":113},6,"Technology",50,"technology",{"id":115,"doc_module":4,"doc_module_name":46,"category_name":116,"show_sort_weight":117,"slug":118},7,"Healthcare",40,"healthcare",{"id":11,"doc_module":4,"doc_module_name":46,"category_name":12,"show_sort_weight":120,"slug":121},30,"research-report",{"id":123,"doc_module":4,"doc_module_name":46,"category_name":124,"show_sort_weight":125,"slug":126},9,"Religion & Spirituality",20,"religion-spirituality",{"id":125,"doc_module":4,"doc_module_name":46,"category_name":128,"show_sort_weight":125,"slug":129},"World Cup","world-cup",{"id":131,"doc_module":4,"doc_module_name":46,"category_name":132,"show_sort_weight":131,"slug":133},10,"Lifestyle","lifestyle",{"id":135,"doc_module":4,"doc_module_name":46,"category_name":136,"show_sort_weight":29,"slug":137},19,"General","general"]