[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-378114-105":59,"doc-detail-378114-en":131},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":124,"head_meta":126,"extra_data":128,"updated_unix":130},105,"en","low-grade-intestinal-inflammation-two-decades-after-pelvic-radiotherapy","Low-grade intestinal inflammation two decades after pelvic radiotherapy","","Radiotherapy can be lifesaving for pelvic cancer, yet it may leave survivors with chronic bowel symptoms whose mechanisms remain unclear. This study investigated whether late-phase intestinal pathology includes inflammation by analyzing mucosal biopsies from irradiated pelvic cancer survivors compared with non-irradiated controls. Twenty-four survivors were profiled using TMT proteomics and mRNA-seq, with immune-cell changes by flow cytometry and mucus barrier integrity via permeability and 16S rRNA bacterial detection.",{"@graph":69,"@context":123},[70,84,106],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/low-grade-intestinal-inflammation-two-decades-after-pelvic-radiotherapy/378114/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/low-grade-intestinal-inflammation-two-decades-after-pelvic-radiotherapy/378114.png","ImageObject",300,407,{"name":92,"@type":93},"Jordan Avery","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-24","2026-09-24",true,{"@type":102,"interactionType":103,"userInteractionCount":105},"InteractionCounter",{"@type":104},"ViewAction",15,{"@type":107,"mainEntity":108},"FAQPage",[109,115,119],{"name":110,"@type":111,"acceptedAnswer":112},"What question does the study address about pelvic radiotherapy after many years?","Question",{"text":113,"@type":114},"Whether the late-phase intestinal pathology after pelvic radiotherapy includes a chronic inflammatory component rather than resolving or progressing only to fibrosis.","Answer",{"name":116,"@type":111,"acceptedAnswer":117},"How were chronic inflammation and tissue changes assessed in the study?",{"text":118,"@type":114},"Mucosal biopsies were analyzed using TMT mass spectrometry and mRNA sequencing to profile proteins and transcripts, with immune-cell populations measured by flow cytometry. Mucus barrier integrity was evaluated by permeability analysis and bacterial detection using 16S rRNA.",{"name":120,"@type":111,"acceptedAnswer":121},"What did the researchers find regarding immune activity and the mucus barrier?",{"text":122,"@type":114},"High-dose exposed mucosa showed differential protein expression consistent with chronic low-grade inflammation involving neutrophil activity. Findings also indicated a weakened mucus barrier with bacterial infiltration.","https://schema.org",{"og:url":83,"og:type":125,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":127,"canonical":83},"index,follow",{"doc_id":129,"site_id":62},378114,1790378174,{"code":4,"msg":5,"data":132},{"doc_id":129,"user_id":133,"nickname":92,"user_avatar":134,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":135,"file_id":136,"file_url":137,"file_type":138,"file_size":139,"view_count":105,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":140,"language":141,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":142,"faqs":143,"seo_title":144,"seo_description":67,"update_tm":145,"read_time":146},1099523882367,"https://ap-avatar.wpscdn.com/davatar_9964176cb1d06d4a9deccf72a44ae3dc","Articles   \nLow-grade intestinal inflammation two decades after pelvic radiotherapy  \nSravani Devarakonda,a Annika Thorsell,b Per Hedenström,c,k Azar Rezapour,d Lisen Heden,e Sanghita Banerjee,f Malin E. V. Johansson,g George Birchenough,g Amelie Toft Morén,a Karin Gustavsson,e Viktor Skokic,a,h Victor L. Pettersson,a Fei Sjöberg,a,j Marie Kalm,i Mohammad Al Masri,a Michaela Ekh,a Henrik Fagman,l Mats Wolving,m Rosie Perkins,c Rodrigo A. Morales,f Francisca Castillo,f Eduardo J. Villablanca,f Ulf Yrlid,d Karin Bergmark,a Gunnar Steineck,a and Cecilia Bulla,∗  \naDivision of Clinical Cancer Epidemiology, Department of Oncology, Institute of Clinical Sciences, The Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden  \nbProteomics Core Facility, The Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden  \ncDepartment of Molecular and Clinical Medicine, Institute of Medicine, University of Gothenburg, Gothenburg, Sweden dDepartment of Microbiology and Immunology, Institute of Biomedicine, The Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden  \nePelvic Cancer Rehabilitation, Sahlgrenska University Hospital, Gothenburg, Sweden  \nfImmunology and Allergy Unit, Department of Medicine, Solna, Karolinska Institute and University Hospital, Stockholm, Sweden gDepartment of Medical Biochemistry and Cell Biology, Institute of Biomedicine, The Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden  \nhDepartment of Molecular Medicine and Surgery and Department of Pelvic Cancer, Karolinska Institute, Stockholm, Sweden iDepartment of Pharmacology, Institute of Neuroscience and Physiology, The Sahlgrenska Academy, University of Gothenburg, 413 90 Gothenburg, Sweden  \njDepartment of Infectious Diseases, Institute of Biomedicine, The Sahlgrenska Academy, University of Gothenburg, 413 90 Gothenburg, Sweden  \nkDepartment of Gastroenterology and Hepatology, Sahlgrenska University Hospital, Gothenburg, Sweden  \nlDepartment of Laboratory Medicine, Institute of Biomedicine, The Sahlgrenska Academy, University of Gothenburg and Department of Clinical Patology, Sahlgrenska University Hospital, Gothenburg, Sweden  \nmDepartment of Pathology, Sahlgrenska University Hospital, Göteborg, Sweden  \nSummary  \nBackground Radiotherapy is effective in the treatment of cancer but also causes damage to non-cancerous tissue. Pelvic radiotherapy may produce chronic and debilitating bowel symptoms, yet the underlying pathophysiology is still undefined. Most notably, although pelvic radiotherapy causes an acute intestinal inflammation there is no consensus on whether the late-phase pathophysiology contains an inflammatory component or not. To address this knowledge gap, we examined the potential presence of a chronic inflammation in mucosal biopsies from irradiated pelvic cancer survivors.  \nMethods We biopsied 24 cancer survivors two to 20 years after pelvic radiotherapy, and four non-irradiated controls. Using tandem mass tag (TMT) mass spectrometry and mRNA sequencing (mRNA-seq), we charted proteomic and transcriptomic profiles of the mucosal tissue previously exposed to a high or a low/no dose of radiation. Changes in the immune cell populations were determined with flow cytometry. The integrity of the protective mucus layers were determined by permeability analysis and 16S rRNA bacterial detection.  \nFindings 942 proteins were differentially expressed in mucosa previously exposed to a high radiation dose compared to a low radiation dose. The data suggested a chronic low-grade inflammation with neutrophil activity, which was confirmed by mRNA-seq and flow cytometry and further supported by findings of a weakened mucus barrier with bacterial infiltration.  \nInterpretation Our results challenge the idea that pelvic radiotherapy causes an acute intestinal inflammation that either heals or turns fibrotic without progression to chronic inflammation. This provides a rationale for exploring novel strategies to mitigate chronic bowel symptoms ","cbCairidtytvYBKd","https://ap.wps.com/l/cbCairidtytvYBKd","pdf",50204029,21,"English","# Summary\n## Background\n## Methods\n## Findings\n## Interpretation\n# Research in context\n## Evidence before this study\n## Added value of this study\n## Implications of all the available evidence","[{\"question\":\"What question does the study address about pelvic radiotherapy after many years?\",\"answer\":\"Whether the late-phase intestinal pathology after pelvic radiotherapy includes a chronic inflammatory component rather than resolving or progressing only to fibrosis.\"},{\"question\":\"How were chronic inflammation and tissue changes assessed in the study?\",\"answer\":\"Mucosal biopsies were analyzed using TMT mass spectrometry and mRNA sequencing to profile proteins and transcripts, with immune-cell populations measured by flow cytometry. Mucus barrier integrity was evaluated by permeability analysis and bacterial detection using 16S rRNA.\"},{\"question\":\"What did the researchers find regarding immune activity and the mucus barrier?\",\"answer\":\"High-dose exposed mucosa showed differential protein expression consistent with chronic low-grade inflammation involving neutrophil activity. Findings also indicated a weakened mucus barrier with bacterial infiltration.\"}]","Low-grade intestinal inflammation two decades after pelvic radiotherapy | PDF",1790228541,53]