[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-detail-333051-en":59,"doc-seo-333051-105":81},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":5,"data":60},{"doc_id":61,"user_id":62,"nickname":63,"user_avatar":64,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":66,"doc_content":67,"file_id":68,"file_url":69,"file_type":70,"file_size":71,"view_count":52,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":72,"language":73,"language_code":74,"site_id":75,"html_lang":74,"table_of_contents":76,"faqs":77,"seo_title":78,"seo_description":66,"update_tm":79,"read_time":80,"preview_page_count":4},333051,1099513958607,"Jiven","https://ap-avatar.wpscdn.com/avatar/100002390cf8733938c?x-image-process=image/resize,m_fixed,w_180,h_180&k=1778829742770036399","Long-read transcriptome landscapes of primary and metastatic liver cancers - research open access","Long-read transcriptome profiling clarifies how primary and metastatic liver cancers differ at the isoform level and why metastasis rewires tumor biology. Using long-read isoform sequencing and short-read RNA sequencing on 95 patients who underwent hepatic resection, the study compares tumor and matched non-tumor liver tissues, focusing on hepatocellular carcinoma, paired primary tumors, and liver metastases. It reports isoform diversity in HCC, metastasis-associated transcriptome changes, metastasis-specific predictors, and immunological and metabolic alterations in adjacent paracancerous tissues.","Chen et al. Biomarker Research (2024) 12:4 Biomarker Research  \n[https://doi.org/10.1186/s40364-023-00554-w](https://doi.org/10.1186/s40364-023-00554-w)  \nRESEARCH Open Access  \nLong-read transcriptome landscapes of primary and metastatic liver cancersat transcript resolution  \nZhiao Chen 1,2,3†, Qili Shi 1†, Yiming Zhao4†, Midie Xu5, Yizhe Liu 1, Xinrong Li 1, Li Liu 1, Menghong Sun5, Xiaohua Wu6, Zhimin Shao2,7, Ye Xu8*, Lu Wang4* and Xianghuo He 1,2,3*  \nAbstract  \nBackground The liver ranks as the sixth most prevalent site of primary cancer in humans, and it frequently experiences metastases from cancers originating in other organs. To facilitate the development of effective treatmentsand improve survival rates, it is crucial to comprehend the intricate and diverse transcriptome landscape of primary and metastatic liver cancers.  \nMethods We conducted long-read isoform sequencing and short-read RNA sequencing using a cohort of 95 patients with primary and secondary liver cancer who underwent hepatic resection. We compared the transcriptome landscapes of primary and metastatic liver cancers and systematically investigated hepatocellular carcinoma (HCC), paired primary tumours and liver metastases, and matched nontumour liver tissues.  \nResults We elucidated the full-length isoform-level transcriptome of primary and metastatic liver cancers in humans. Our analysis revealed isoform-level diversity in HCC and identified transcriptome variations associated with liver metastatis. Specific RNA transcripts and isoform switching events with clinical implications were profound in liver cancer. Moreover, we defined metastasis-specific transcripts that may serve as predictors of risk of metastasis. Additionally, we observed abnormalities in adjacent paracancerous liver tissues and characterized the immunological and metabolic alterations occurring in the liver.  \nConclusions Our findings underscore the power of full-length transcriptome profiling in providing novel biological insights into the molecular mechanisms underlying tumourigenesis. These insights will further contribute to improving treatment strategies for primary and metastatic liver cancers.  \n†Zhiao Chen, Qili Shi, andYiming Zhao contributed equally to this article. Corresponding Authors: Xianghuo He; Lu Wang; Ye Xu.  \n*Correspondence:  \nYe Xu [xuye021@163.com](xuye021@163.com)[ ](xuye021@163.com)Lu Wang [wangluzl@fudan.edu.cn](wangluzl@fudan.edu.cn)[ ](wangluzl@fudan.edu.cn)Xianghuo He[xhhe@fudan.edu.cn](xhhe@fudan.edu.cn)  \nFull list of author information is available at the end of the article  \n© The Author(s) 2024. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit [http://creativecommons.org/l](http://creativecommons.org/l)icenses/by/4.0/. The Creative Commons Public Domain Dedication waiver ([http://creativecommons.org/publicdomain/zero/1.0/](http://creativecommons.org/publicdomain/zero/1.0/)) applies to the data made available in this article, unless otherwise stated in a credit line to the data.  \nChen et al. Biomarker Research (2024) 12:4 Page 2 of 22  \nKeywords Third-generation sequencing technology, Specific RNA transcript, Isoform switch, Metastasis-specific transcript, Immunosuppressive environment  \nBackground  \nLiver","cbCaiiXgGOhliGls","https://ap.wps.com/l/cbCaiiXgGOhliGls","pdf",13163067,22,"English","en",105,"# Abstract\n## Background\n## Methods\n## Results\n## Conclusions\n# Keywords\n# Background","[{\"question\":\"What question does the study address about liver cancer transcriptomes?\",\"answer\":\"It investigates the intricate and diverse transcriptome landscape differences between primary and metastatic liver cancers, emphasizing isoform-level changes and metastasis-associated molecular mechanisms.\"},{\"question\":\"How were transcriptomes analyzed in this study?\",\"answer\":\"Long-read isoform sequencing and short-read RNA sequencing were performed on a cohort of 95 patients with primary and secondary liver cancer after hepatic resection.\"},{\"question\":\"What key biological findings are reported for primary versus metastatic disease?\",\"answer\":\"The study elucidates full-length isoforms, identifies isoform-level diversity in HCC, detects transcriptome variations linked to metastasis, defines metastasis-specific transcripts as potential predictors, and characterizes immunological and metabolic alterations in adjacent paracancerous tissues.\"}]","Long-read transcriptome landscapes of primary and metastatic liver cancers - research open access | PDF",1790000579,55,{"code":4,"msg":82,"data":83},"ok",{"site_id":75,"language":74,"slug":84,"title":65,"keywords":85,"description":66,"schema_data":86,"social_meta":140,"head_meta":142,"extra_data":144,"updated_unix":145},"long-read-transcriptome-landscapes-of-primary-and-metastatic-liver-cancers-research-open-access","",{"@graph":87,"@context":139},[88,102,122],{"@type":89,"itemListElement":90},"BreadcrumbList",[91,95,97,100],{"item":92,"name":93,"@type":94,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":96,"name":9,"@type":94,"position":14},"https://docshare.wps.com/document/",{"item":98,"name":40,"@type":94,"position":99},"https://docshare.wps.com/document/research-report/",3,{"item":101,"name":65,"@type":94,"position":19},"https://docshare.wps.com/document/long-read-transcriptome-landscapes-of-primary-and-metastatic-liver-cancers-research-open-access/333051/",{"url":101,"name":65,"@type":103,"image":104,"author":109,"headline":65,"publisher":111,"fileFormat":114,"inLanguage":74,"description":66,"dateModified":115,"datePublished":116,"encodingFormat":114,"isAccessibleForFree":117,"interactionStatistic":118},"DigitalDocument",{"url":105,"@type":106,"width":107,"height":108},"https://docshare.wps.com/thumbnails/long-read-transcriptome-landscapes-of-primary-and-metastatic-liver-cancers-research-open-access/333051.png","ImageObject",300,407,{"name":63,"@type":110},"Person",{"url":92,"name":112,"@type":113},"DocShare","Organization","application/pdf","2026-11-13","2026-09-21",true,{"@type":119,"interactionType":120,"userInteractionCount":52},"InteractionCounter",{"@type":121},"ViewAction",{"@type":123,"mainEntity":124},"FAQPage",[125,131,135],{"name":126,"@type":127,"acceptedAnswer":128},"What question does the study address about liver cancer transcriptomes?","Question",{"text":129,"@type":130},"It investigates the intricate and diverse transcriptome landscape differences between primary and metastatic liver cancers, emphasizing isoform-level changes and metastasis-associated molecular mechanisms.","Answer",{"name":132,"@type":127,"acceptedAnswer":133},"How were transcriptomes analyzed in this study?",{"text":134,"@type":130},"Long-read isoform sequencing and short-read RNA sequencing were performed on a cohort of 95 patients with primary and secondary liver cancer after hepatic resection.",{"name":136,"@type":127,"acceptedAnswer":137},"What key biological findings are reported for primary versus metastatic disease?",{"text":138,"@type":130},"The study elucidates full-length isoforms, identifies isoform-level diversity in HCC, detects transcriptome variations linked to metastasis, defines metastasis-specific transcripts as potential predictors, and characterizes immunological and metabolic alterations in adjacent paracancerous tissues.","https://schema.org",{"og:url":101,"og:type":141,"og:title":65,"og:site_name":112,"og:description":66},"article",{"robots":143,"canonical":101},"index,follow",{"doc_id":61,"site_id":75},1790171550]