[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"doc-seo-383503-105":3,"detail-sidebar-cat-0-en-105":81,"doc-detail-383503-en":130},{"code":4,"msg":5,"data":6},0,"ok",{"site_id":7,"language":8,"slug":9,"title":10,"keywords":11,"description":12,"schema_data":13,"social_meta":74,"head_meta":76,"extra_data":78,"updated_unix":80},105,"en","lncrna-pvt1-influences-breast-cancer-cells-glycolysis-through-sponging-mir-145-5p","LncRNA PVT1 influences breast cancer cells glycolysis through sponging miR-145-5p","","Long non-coding RNA PVT1 is an oncogenic regulatory ncRNA implicated in multiple cancers, yet its role in breast cancer remained unclear. This study assessed PVT1 expression in TCGA breast cancer data and tested PVT1 overexpression or knockdown in MDAMB-231 cells. Functional assays measured proliferation, apoptosis, invasion and migration, while ECAR evaluated glycolysis metabolism. Xenografts examined tumor effects in vivo. Results showed PVT1 upregulation and inverse miR-145-5p association, linking PVT1 to glycolysis-driven proliferation and metastasis.",{"@graph":14,"@context":73},[15,34,56],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/healthcare/","Healthcare",3,{"item":32,"name":10,"@type":21,"position":33},"https://docshare.wps.com/document/lncrna-pvt1-influences-breast-cancer-cells-glycolysis-through-sponging-mir-145-5p/383503/",4,{"url":32,"name":10,"@type":35,"image":36,"author":41,"headline":10,"publisher":44,"fileFormat":47,"inLanguage":8,"description":12,"dateModified":48,"datePublished":49,"encodingFormat":47,"isAccessibleForFree":50,"interactionStatistic":51},"DigitalDocument",{"url":37,"@type":38,"width":39,"height":40},"https://docshare.wps.com/thumbnails/lncrna-pvt1-influences-breast-cancer-cells-glycolysis-through-sponging-mir-145-5p/383503.png","ImageObject",300,407,{"name":42,"@type":43},"Liam","Person",{"url":19,"name":45,"@type":46},"DocShare","Organization","application/pdf","2026-10-30","2026-09-24",true,{"@type":52,"interactionType":53,"userInteractionCount":55},"InteractionCounter",{"@type":54},"ViewAction",9,{"@type":57,"mainEntity":58},"FAQPage",[59,65,69],{"name":60,"@type":61,"acceptedAnswer":62},"What role does lncRNA PVT1 play in breast cancer glycolysis according to the study?","Question",{"text":63,"@type":64},"PVT1 modulates glycolysis-related gene expression and shifts tumor metabolic phenotype, assessed with ECAR. Higher PVT1 activity is associated with enhanced glycolysis-related tumor behavior.","Answer",{"name":66,"@type":61,"acceptedAnswer":67},"How was PVT1 function validated experimentally?",{"text":68,"@type":64},"The study used TCGA for expression correlation and used PVT1 overexpression and knockdown lentivirus to infect MDAMB-231 cells. It evaluated proliferation (CCK-8), apoptosis (flow cytometry), invasion and migration (transwell), and glycolysis (ECAR), plus an in vivo xenograft model.",{"name":70,"@type":61,"acceptedAnswer":71},"What is the relationship between PVT1 and miR-145-5p?",{"text":72,"@type":64},"miR-145-5p expression is negatively correlated with PVT1. The authors propose that PVT1 promotes breast cancer progression by competitively sponging miR-145-5p.","https://schema.org",{"og:url":32,"og:type":75,"og:title":10,"og:site_name":45,"og:description":12},"article",{"robots":77,"canonical":32},"index,follow",{"doc_id":79,"site_id":7},383503,1790293606,{"code":4,"msg":82,"data":83},"success",[84,88,92,96,101,106,110,115,119,122,126],{"id":22,"doc_module":4,"doc_module_name":25,"category_name":85,"show_sort_weight":86,"slug":87},"Story & Novel",90,"story-novel",{"id":26,"doc_module":4,"doc_module_name":25,"category_name":89,"show_sort_weight":90,"slug":91},"Literature",80,"literature",{"id":33,"doc_module":4,"doc_module_name":25,"category_name":93,"show_sort_weight":94,"slug":95},"Exam",70,"exam",{"id":97,"doc_module":4,"doc_module_name":25,"category_name":98,"show_sort_weight":99,"slug":100},5,"Comic",60,"comic",{"id":102,"doc_module":4,"doc_module_name":25,"category_name":103,"show_sort_weight":104,"slug":105},6,"Technology",50,"technology",{"id":107,"doc_module":4,"doc_module_name":25,"category_name":29,"show_sort_weight":108,"slug":109},7,40,"healthcare",{"id":111,"doc_module":4,"doc_module_name":25,"category_name":112,"show_sort_weight":113,"slug":114},8,"Research & Report",30,"research-report",{"id":55,"doc_module":4,"doc_module_name":25,"category_name":116,"show_sort_weight":117,"slug":118},"Religion & Spirituality",20,"religion-spirituality",{"id":117,"doc_module":4,"doc_module_name":25,"category_name":120,"show_sort_weight":117,"slug":121},"World Cup","world-cup",{"id":123,"doc_module":4,"doc_module_name":25,"category_name":124,"show_sort_weight":123,"slug":125},10,"Lifestyle","lifestyle",{"id":127,"doc_module":4,"doc_module_name":25,"category_name":128,"show_sort_weight":97,"slug":129},19,"General","general",{"code":4,"msg":82,"data":131},{"doc_id":79,"user_id":132,"nickname":42,"user_avatar":133,"doc_module":4,"category_id":107,"category_name":29,"doc_title":10,"doc_description":12,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":55,"is_deleted":4,"is_public":22,"is_downloadable":22,"audit_status":22,"page_count":139,"language":140,"language_code":8,"site_id":7,"html_lang":8,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":12,"update_tm":144,"read_time":113},8796095461564,"https://ap-avatar.wpscdn.com/davatar_155a257f0dc6eb9ab79c44ca47cae57d","Genes & Genomics (2023) 45:581–592  \n[https://doi.org/10.1007/s13258-023-01368-8](https://doi.org/10.1007/s13258-023-01368-8)  \nOnline ISSN 2092-9293 Print ISSN 1976-9571  \nLncRNA PVT1 influences breast cancer cells glycolysis  \nthrough sponging miR‑145‑5p  \nHuan Qu1,2 · Xingxing Li1 · Fei Chen1 · Min Zhang1 · Xun Lu3 · Yun Gu4 · Mingming Lv1 · Cheng Lu1  \nReceived: 28 September 2022 / Accepted: 27 January 2023 / Published online: 20 March 2023 © The Author(s) 2023  \nAbstract  \nBackground Long-non-coding RNA PVT1 (lncRNA PVT1) can be used as an oncogenic regulatory non-coding RNA (ncRNA) for many cancers. However, its function and mechanism in breast cancer (BRCA) are still not clearly elucidated. Objective We attempt to explain the mechanism of PVT1’s role in breast cancer from different perspectives.  \nMethods We analyzed the expression of PVT1 and its correlation with the breast cancer related clinical data in the The Cancer Genome Atlas (TCGA) database. We used PVT1 overexpression and knockdown lentivirus to infect breast cancer MDAMB-231 cell line for cell function verification, in vitro using CCK-8 to measure proliferation, flow cytometry to measure apoptosis, transwell test to measure invasion and migration ability, detecting cell extracellular acidification rate (ECAR) to assess glycolysis metabolism and explore the biological functions of PVT1 in breast cancer cells. Transcriptome sequencing was used to analyze the changes of related genes in cells after overexpression of PVT1 . In vivo we used a xenograft model to study the effect of PVT1 on breast cancer.  \nResults PVT1 was up-regulated in breast cancer tissues and was positively correlated with the clinical stage of breast cancer patients. Overexpression of PVT1 in vitro promoted cell proliferation, migration and invasion, and promoted tumor growth in vivo. Knockdown of PVT1 led to the opposite biological consequence. Further bioinformatics analysis showed that PVT1 changes the glycolysis metabolism of tumors through regulation of glycolysis-related genes. In addition, the expression of miR-145-5p is negatively correlated with PVT1 . We consider the possibility of PVT1 promoting cell proliferation and metastasis by regulating the aerobic glucose metabolism in breast cancer cells through sponging the miR-145-5p. Conclusion Our results reveal a potential pathway for competing endogenous RNA to regulate breast cancer glucose metabolism. PVT1 regulates glycolysis related genes expression by competitively binding to endogenous miR-145-5p in breast cancer cells to change the metabolic phenotype. This may Provide new ideas for precise molecular therapy targets for breast cancer.  \nKeywords Breast cancer · LncRNA PVT1 · miR-145-5p · Glycolysis  \n* Mingming Lv [jinanmingming@126.com](jinanmingming@126.com)  \n* Cheng Lu[lucheng_njmu@163.com](lucheng_njmu@163.com)  \n1 Department of Breast, Women’s Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, 123rd Tianfei Street, Mochou Road, Nanjing 210004, China  \n2 Department of Health Management Centre, School of Medicine, Zhongda Hospital, Southeast University, Nanjing 210009, China  \n3 School of Public Health, Yale University, New Haven, CT 06520, USA  \n4 Department of Pathology, Nanjing Maternity and Child Health Care Hospital, Women’s Hospital of Nanjing Medical University, Nanjing 210004, China  \nIntroduction  \nBreast cancer has always been a common malignant tumor for women worldwide. The latest cancer statistics showed that breast cancer accounts the most number of new onset cancers in women (Siegel et al. 2021) . The high incidence of breast cancer not only severely damages women’s health and lives, but also imposes an immeasurable economic burden on the family and society (Sung et al. 2021) . It is an urgent public health problem facing society today. Due to chemotherapy resistance and endocrine therapy resistance, the overall survival rate of breast cancer patients still needs to be i","cbCaivyq79Ma05cF","https://ap.wps.com/l/cbCaivyq79Ma05cF","pdf",11718695,12,"English","# Abstract\n## Background\n## Objective\n## Methods\n## Results\n## Conclusion\n# Introduction\n## Rationale and clinical need\n## Biological context of lncRNAs and PVT1\n## Study rationale in breast cancer\n# Materials and methods\n## Cell culture and stabl","[{\"question\":\"What role does lncRNA PVT1 play in breast cancer glycolysis according to the study?\",\"answer\":\"PVT1 modulates glycolysis-related gene expression and shifts tumor metabolic phenotype, assessed with ECAR. Higher PVT1 activity is associated with enhanced glycolysis-related tumor behavior.\"},{\"question\":\"How was PVT1 function validated experimentally?\",\"answer\":\"The study used TCGA for expression correlation and used PVT1 overexpression and knockdown lentivirus to infect MDAMB-231 cells. It evaluated proliferation (CCK-8), apoptosis (flow cytometry), invasion and migration (transwell), and glycolysis (ECAR), plus an in vivo xenograft model.\"},{\"question\":\"What is the relationship between PVT1 and miR-145-5p?\",\"answer\":\"miR-145-5p expression is negatively correlated with PVT1. The authors propose that PVT1 promotes breast cancer progression by competitively sponging miR-145-5p.\"}]","LncRNA PVT1 influences breast cancer cells glycolysis through sponging miR-145-5p | PDF",1790257341]