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Expression of HOXC-AS3 and KDM5B is assessed by qRT-PCR and Western blot, followed by functional proliferation assays in vitro and xenograft validation in vivo. Results show nuclear HOXC-AS3 directly binds KDM5B, suppresses TRIM37-mediated KDM5B ubiquitination, and stabilizes KDM5B protein levels, promoting tumor growth; KDM5B overexpression counteracts HOXC-AS3 silencing.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/lncrna-hoxc-as3-accelerates-malignant-proliferation-of-cervical-cancer-cells-via-stabilizing-kdm5b/330789/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/lncrna-hoxc-as3-accelerates-malignant-proliferation-of-cervical-cancer-cells-via-stabilizing-kdm5b/330789.png","ImageObject",300,407,{"name":92,"@type":93},"Hazel","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-26","2026-09-21",true,{"@type":102,"interactionType":103,"userInteractionCount":24},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What role does lncRNA HOXC-AS3 play in cervical cancer cell proliferation?","Question",{"text":112,"@type":113},"lncRNA HOXC-AS3 is upregulated in cervical cancer tissues and cells, and its depletion represses cervical cancer cell proliferation and in vivo tumor growth.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How does HOXC-AS3 regulate KDM5B?",{"text":117,"@type":113},"HOXC-AS3 localizes to the nucleus and directly binds KDM5B, inhibiting TRIM37-mediated ubiquitination of KDM5B and upregulating/stabilizing KDM5B protein levels.",{"name":119,"@type":110,"acceptedAnswer":120},"What experimental approaches were used to validate the findings?",{"text":121,"@type":113},"The study measures HOXC-AS3 and KDM5B expression by qRT-PCR and Western blot, evaluates proliferation with CCK-8, colony formation, and 5-Ethynyl-2'-deoxyuridine staining, and confirms in vivo effects using a xenograft tumor model with tumor size/weight and immunohistochemistry.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},330789,1790174386,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":24,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145,"preview_page_count":4},137441390410,"https://ap-avatar.wpscdn.com/avatar/2000252f4ab5702993?_k=1776741390130283984","Journal of Cancer Research and Clinical Oncology (2024) 150:294  \n[https://doi.org/10.1007/s00432-024-05799-y](https://doi.org/10.1007/s00432-024-05799-y)  \nLncRNA HOXC‑AS3 accelerates malignant proliferation of cervical cancer cells via stabilizing KDM5B  \nJie Li1 · Fang Hou1 · Zhenghua Teng1 · Weiwei Xia1 · Jie Peng1  \nReceived: 9 January 2024 / Accepted: 10 May 2024 / Published online: 6 June 2024 © The Author(s) 2024  \nAbstract  \nBackground Cervical cancer (CC) is a common malignancy amongst women globally. Ubiquitination plays a dual role in the occurrence and development of cancers. This study analyzed the mechanism of long noncoding RNA HOXC cluster antisense RNA 3 (lncRNA HOXC-AS3) in malignant proliferation of CC cells via mediating ubiquitination of lysine demethylase 5B (KDM5B/JARID1B) .  \nMethods The expression patterns of lncRNA HOXC-AS3 and KDM5B were measured by real-time quantitative polymerase chain reaction or Western blot analysis. After transfection with lncRNA HOXC-AS3 siRNA and pcDNA3.1-KDM5B, proliferation of CC cells was assessed by the cell counting kit-8, colony formation, and 5-Ethynyl-2’-deoxyuridine staining assays. The xenograft tumor model was established to confirm the impact of lncRNA HOXC-AS3 on CC cell proliferation in vivo by measuring tumor size and weight and the immunohistochemistry assay. The subcellular location of lncRNA HOXCAS3 and the binding of lncRNA HOXC-AS3 to KDM5B were analyzed. After treatment of lncRNA HOXC-AS3 siRNA or MG132, the protein and ubiquitination levels of KDM5B were determined. Thereafter, the interaction and the subcellular co-location of tripartite motif-containing 37 (TRIM37) and KDM5B were analyzed by the co-immunoprecipitation and immunofluorescence assays.  \nResults LncRNA HOXC-AS3 and KDM5B were upregulated in CC tissues and cells. Depletion of lncRNA HOXC-AS3 repressed CC cell proliferation and in vivo tumor growth. Mechanically, lncRNA HOXC-AS3 located in the nucleus directly bound to KDM5B, inhibited TRIM37-mediated ubiquitination of KDM5B, and upregulated the protein levels of KDM5B. KDM5B overexpression attenuated the inhibitory role of silencing lncRNA HOXC-AS3 in CC cell proliferation in vivo and in vitro.  \nConclusion Nucleus-located lncRNA HOXC-AS3 facilitated malignant proliferation of CC cells via stabilization of KDM5B protein levels.  \nKeywords Cervical cancer · LncRNA HOXC-AS3 · KDM5B · Proliferation · TRIM37  \nBackground  \nCervical cancer (CC), primarily including cervical squamous cell carcinoma (over 70% of all CC cases) and cervical adenocarcinoma (over 25% of all CC cases), is recognized  \nJie Li and Fang Hou have contributed equally to this work and should be considered co-first authors.  \n* Jie Peng [pengjie_08@163.com](pengjie_08@163.com)  \n1 Department of Obstetrics and Gynecology, Suzhou Wuzhong People’s Hospital, Jiangsu Province, No. 61, Dongwu North Road, Suzhou City 215128, China  \nas a commonly diagnosed malignant tumor in gynecology associated with a high occurrence for the reason of persistent infections with high risk human papillomavirus (HPV), threatening the health status of women all over the world (Johnson et al. 2019) . In 2020, an estimated number of over 604,127 new cases of CC are diagnosed and over 341,831 deaths occur as a result of this debilitating malignancy (Sunget al. 2021) . Despite the ongoing implements of treatment modalities, such as surgery, radiotherapy, and chemotherapy, the resistance, metastasis, and recurrence of CC lead to unsatisfactory overall survival rates of CC patients (Feng et al. 2020 ; Adiga et al. 2021) . Therefore, an in-depth investigation of malignant proliferation of CC cells is instrumental in understanding the occurrence and progression of CC.  \nLong noncoding RNAs (lncRNAs), a cluster of regulatory noncoding RNAs consisting of transcripts over 200 nucleotides, mediate gene expression in a transcriptional and/or post-transcriptional manner and exhibit biological functions in a myria","cbCaikQIBXwBdzwP","https://ap.wps.com/l/cbCaikQIBXwBdzwP","pdf",8247207,15,"English","# Abstract\n## Background\n## Methods\n## Results\n## Conclusion\n# Keywords\n# Background\n## Epidemiology and treatment challenges\n## Roles of lncRNAs in cancer\n## Role of KDM5B and ubiquitination","[{\"question\":\"What role does lncRNA HOXC-AS3 play in cervical cancer cell proliferation?\",\"answer\":\"lncRNA HOXC-AS3 is upregulated in cervical cancer tissues and cells, and its depletion represses cervical cancer cell proliferation and in vivo tumor growth.\"},{\"question\":\"How does HOXC-AS3 regulate KDM5B?\",\"answer\":\"HOXC-AS3 localizes to the nucleus and directly binds KDM5B, inhibiting TRIM37-mediated ubiquitination of KDM5B and upregulating/stabilizing KDM5B protein levels.\"},{\"question\":\"What experimental approaches were used to validate the findings?\",\"answer\":\"The study measures HOXC-AS3 and KDM5B expression by qRT-PCR and Western blot, evaluates proliferation with CCK-8, colony formation, and 5-Ethynyl-2'-deoxyuridine staining, and confirms in vivo effects using a xenograft tumor model with tumor size/weight and immunohistochemistry.\"}]","LncRNA HOXC-AS3 accelerates malignant proliferation of cervical cancer cells via stabilizing KDM5B | PDF",1789990134,38]