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FOXP4-AS1 knockdown suppresses migration, invasion, and EMT, and FOXP4-AS1 silencing reduces tumor growth in vivo. Mechanistically, FOXP4-AS1 acts as a scaffold binding USP7 and ZEB1, regulating ZEB1 ubiquitination and expression.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/lncrna-foxp4-as1-facilitates-colorectal-cancer-invasion-and-migration-read-online-free/346382/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/lncrna-foxp4-as1-facilitates-colorectal-cancer-invasion-and-migration-read-online-free/346382.png","ImageObject",300,407,{"name":92,"@type":93},"Himbo","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-09-23","2026-09-22",true,{"@type":102,"interactionType":103,"userInteractionCount":14},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"How is FOXP4-AS1 expression related to colorectal cancer in this study?","Question",{"text":112,"@type":113},"FOXP4-AS1 expression is markedly elevated in CRC specimens and cells. The study then uses this differential expression to explore functional consequences.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"What effects does FOXP4-AS1 knockdown have on CRC cells?",{"text":117,"@type":113},"FOXP4-AS1 knockdown suppresses CRC cell migration, invasion, and EMT. In vivo, FOXP4-AS1 silencing weakens CRC tumor growth.",{"name":119,"@type":110,"acceptedAnswer":120},"What mechanism links FOXP4-AS1 to metastasis through USP7 and ZEB1?",{"text":121,"@type":113},"FOXP4-AS1 functions as a scaffold that binds both USP7 and ZEB1, regulating ZEB1 ubiquitination and expression. USP7 inhibition with P005091 blocks FOXP4-AS1-driven promotion of metastasis, and ZEB1 overexpression reverses the effects of FOXP4-AS1 silencing.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},346382,1790158730,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":14,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145},687197100911,"https://ap-avatar.wpscdn.com/avatar/a000239b6f1da00475?x-image-process=image/resize,m_fixed,w_180,h_180&k=1785132997149421697","[www. nature.com/scientificreports](www. nature.com/scientificreports)  \nOPEN  \nLncRNA FOXP4-AS1 facilitates colorectal cancer invasion and migration by enhancing USP7 interaction with ZEB1  \nXiaoling Yang1,4,5, Chenglong Shen1,4,5, Yuchen Yuan2, Jiazhe Shao1,4,5, Haichen Liu1,4, Yichen Li1,4,5, Guoqiang Zhou1,3,4􀀍 & Zhiliang Shi1,3,4􀀍  \nColorectal cancer (CRC) poses a threat to the health of people worldwide. Long noncoding RNAs (lncRNAs) have been reported to play a key role in regulating carcinogenesis, including CRC. In this study, the levels of lncRNA FOXP4-AS1 were analyzed inCRC specimens and cells via qRT-PCR. The impacts of FOXP4-AS1 on CRC cell metastasis were investigated. Then, the silver staining assay, western blot, RIP, Co-IP, and immunofluorescence were conducted to explore and validate the molecular mechanisms by which FOXP4-AS1 affects CRC progression. We discovered that FOXP4-AS1 expression was markedly elevated inCRC. Functionally, FOXP4-AS1 knockdown suppressed CRC cell migration, invasion, and EMT. Also, FOXP4-AS1 silencing weakened CRC tumor growth in vivo. Mechanistically, we identified that FOXP4-AS1 functioned as a scaffold to simultaneously bind USP7 and ZEB1, and regulated the ubiquitination and expression of ZEB1 by binding to USP7 . Rescue experiments demonstrated that USP7 inhibitor P005091 abolished the promotion of cell metastasis by FOXP4-AS1 overexpression. Furthermore, ZEB1 overexpression reversed the impact of silencing FOXP4-AS1 on cell metastasis. Collectively, our work revealed the molecular mechanism and role of FOXP4-AS1-mediated USP7-ZEB1 axis inCRC.  \nKeywords LncRNA FOXP4-AS1, USP7, ZEB1, Colorectal cancer  \nColorectal cancer (CRC) is one of the common and serious malignant tumors threatening human life and health, with the third highest incidence and second highest mortality rate among various malignant tumors worldwide1. Multiple risk factors are associated with the development of the disease, such as, genetic inheritance and poor lifestyle habits2. Currently, the clinical treatment of CRC is mainly a comprehensive treatment based on surgery, and there is a significant improvement in the clinical outcome of CRC. However, the treatment of patients with advanced stage, post-surgical recurrence, and resistance is still the difficulty of clinical treatment, and the 5-year survival rate has not been obviously improved3. Therefore, finding new early diagnostic markers for CRC, elucidating the mechanisms ofCRC development, and identifying new therapeutic targets are the keys to inhibiting CRC tumor progression and reducing recurrence and mortality.  \nThe development of malignant tumors is an extremely complex biological phenomenon that is multifactorial, multigenic, and undergoes multiple stages before it finally develops. Both inactivation and activation of oncogenes cause genetic alterations thereby affecting various aspects of tumors, such as proliferation, invasion, metastasis and material metabolism4. Previous studies on tumor-related genes have been focused on coding protein genes, and with the continuous development and improvement of technologies such as high-throughput sequencing, a large number of long fragments of non-coding RNAs have been uncovered, and their significance in life activities has been gradually revealed5. Long noncoding RNAs (lncRNAs) are a class of molecules that do not encode proteins but have biological functions. LncRNAs are dysregulated in a large of human diseases, including CRC, and their dysregulation is intimately related to disease progression, suggesting that they have potential clinical application value6. LncRNAs act through kinds of mechanisms, mainly by interacting with  \n1Department of Gastrointestinal Surgery, Affiliated Changshu Hospital of Nantong University, Changshu 215500, Jiangsu Province, China. 2Department of General Surgery, Meili People’s Hospital, Changshu 215500, Jiangsu Province, China. 3Gusu College, Nanjing Medical University, Su","cbCaijTB4ELqaaFH","https://ap.wps.com/l/cbCaijTB4ELqaaFH","pdf",17480017,13,"English","# Introduction\n## CRC clinical challenge and unmet needs\n## Oncogenic mechanisms and lncRNA relevance to CRC\n# Methods and Study Aim\n## Study objectives across clinical, molecular, and animal levels\n# Results\n## FOXP4-AS1 expression in CRC tissues and cells\n## Functional effects on migration, invasion, EMT, and tumor growth\n# Mechanism\n## FOXP4-AS1 scaffold role in USP7-ZEB1 axis\n# Validation Experiments\n## Rescue with USP7 inhibitor and ZEB1 overexpression","[{\"question\":\"How is FOXP4-AS1 expression related to colorectal cancer in this study?\",\"answer\":\"FOXP4-AS1 expression is markedly elevated in CRC specimens and cells. The study then uses this differential expression to explore functional consequences.\"},{\"question\":\"What effects does FOXP4-AS1 knockdown have on CRC cells?\",\"answer\":\"FOXP4-AS1 knockdown suppresses CRC cell migration, invasion, and EMT. In vivo, FOXP4-AS1 silencing weakens CRC tumor growth.\"},{\"question\":\"What mechanism links FOXP4-AS1 to metastasis through USP7 and ZEB1?\",\"answer\":\"FOXP4-AS1 functions as a scaffold that binds both USP7 and ZEB1, regulating ZEB1 ubiquitination and expression. USP7 inhibition with P005091 blocks FOXP4-AS1-driven promotion of metastasis, and ZEB1 overexpression reverses the effects of FOXP4-AS1 silencing.\"}]","LncRNA FOXP4-AS1 facilitates colorectal cancer invasion and migration - read online free | PDF",1790061210,33]