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Cancer Cells  \nPablo Pérez-Moreno 1, Ismael Riquelme 2, Carolina Bizama 3, Luis Vergara-Gómez 4, Julio C. Tapia 5, Priscilla Brebi 6, Patricia García 3 and Juan Carlos Roa 3, *  \nCitation: Pérez-Moreno, P.; Riquelme, I.; Bizama, C.; Vergara-Gómez, L.; Tapia, J.C.; Brebi, P.; García, P.; Roa, J.C. LINC00662 Promotes Aggressive Traits by Modulating OCT4  \nExpression through miR-335-5p in Gallbladder Cancer Cells. Int. J. Mol. Sci. 2024, 25, 6740. [https://](https://)[ ](https://)[doi.org/10.3390/ijms25126740](doi.org/10.3390/ijms25126740)  \nAcademic Editor: Nam Deuk Kim  \nReceived: 16 March 2024  \nRevised: 14 June 2024  \nAccepted: 14 June 2024  \nPublished: 19 June 2024  \nCopyright: © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ([https://](https://)[ ](https://)[creativecommons.org/licenses/by/](creativecommons.org/licenses/by/)[ ](creativecommons.org/licenses/by/)[4.0/](4.0/)) .  \n1 Programa de Comunicación Celular en Cáncer, Facultad de Medicina Clínica Alemana, Universidad del Desarrollo, Santiago 7780272, Chile; [p.perez@udd.cl](p.perez@udd.cl)  \n2 Institute of Biomedical Sciences, Faculty of Health Sciences, Universidad Autónoma de Chile, Temuco 4810101, Chile; [ismael.riquelme@uautonoma.cl](ismael.riquelme@uautonoma.cl)  \n3 Millenium Institute on Immunology and Immunotherapy (IMII), Centro de Prevención y Control de Cancer (CECAN), Department of Pathology, School of Medicine, Pontificia Universidad Católica de Chile, Santiago 8380000, Chile; [cbizamas@uc.cl](cbizamas@uc.cl) (C.B.); [pgarciam@uc.cl](pgarciam@uc.cl) (P.G.)  \n4 Centre of Excellence in Translational Medicine (CEMT), Scientific and Technological Bioresource Nucleus (BIOREN), Biomedicine and Translational Research Lab, Universidad de la Frontera, Temuco 4810296, Chile; [luisosvaldo.vergara@ufrontera.cl](luisosvaldo.vergara@ufrontera.cl)  \n5 Programa de Biología Celular y Molecular, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago 8380453, Chile; [jtapiapineda@uchile.cl](jtapiapineda@uchile.cl)  \n6 Laboratory of Integrative Biology (LIBi), Millennium Institute on Immunology and Immunotherapy (MIII), Center for Excellence in Translational Medicine—Scientific and Technological Bioresource  \nNucleus (CEMT-BIOREN), Universidad de La Frontera, Temuco 4810296, Chile; [priscilla.brebi@ufrontera.cl](priscilla.brebi@ufrontera.cl)  \n* Correspondence: [croa@uc.cl](croa@uc.cl); Tel.: +56-22354-1061  \nAbstract: Long non-coding RNAs (lncRNAs) are nucleotide sequences that participate in different biological processes and are associated with different pathologies, including cancer. Long intergenic non-protein-coding RNA 662 (LINC00662) has been reported to be involved in different cancers, including colorectal, prostate, and breast cancer. However, its role in gallbladder cancer has not yet been described. In this article, we hypothesize that LINC00662 has an important role in the acquisition of aggressiveness traits such as a stem-like phenotype, invasion, and chemoresistance in gallbladder cancer. Here, we show that LINC00662 is associated with larger tumor size and lymph node metastasis in patients with gallbladder cancer. Furthermore, we show that the overexpression of LINC00662 promotes an increase in CD133+ /CD44+ cell populations and the expression of stemness-associated genes. LINC00662 promotes greater invasive capacity and the expression of genes associated with epithelial–mesenchymal transition. In addition, the expression of LINC00662 promotes resistance tocisplatin and 5-fluorouracil, associated with increased expression of chemoresistance-related ATPbinding cassette (ABC) transporters in gallbladder cancer (GBC) cell lines. Finally, we show that the mechanism by which LINC006","cbCaimo5AV8eto7h","https://ap.wps.com/l/cbCaimo5AV8eto7h","pdf",2411177,13,"English","# Introduction\n## Background on gallbladder cancer\n## Role of non-coding RNAs in cancer\n## LncRNA characteristics and ceRNA mechanism","[{\"question\":\"What is the main focus of the study about LINC00662 in gallbladder cancer?\",\"answer\":\"The study investigates how LINC00662 influences aggressive traits in gallbladder cancer, including stem-like phenotype, invasion, and drug resistance.\"},{\"question\":\"How does LINC00662 affect tumor behavior in patients?\",\"answer\":\"LINC00662 is associated with larger tumor size and lymph node metastasis in gallbladder cancer patients.\"},{\"question\":\"What molecular mechanism does the article propose for LINC00662’s function?\",\"answer\":\"The proposed mechanism involves decreasing miR-335-5p and increasing OCT4 expression in gallbladder cancer cells.\"}]","LINC00662 Promotes Aggressive Traits by Modulating OCT4 Expression through miR-335-5p in Gallbladder Cancer Cells | PDF",1790021100,33]