[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"doc-seo-331045-105":3,"detail-sidebar-cat-0-en-105":81,"doc-detail-331045-en":130},{"code":4,"msg":5,"data":6},0,"ok",{"site_id":7,"language":8,"slug":9,"title":10,"keywords":11,"description":12,"schema_data":13,"social_meta":74,"head_meta":76,"extra_data":78,"updated_unix":80},105,"en","lets-make-it-personal-crispr-tools-in-manipulating-cell-death-pathways-for-cancer-treatment","Let’s make it personal: CRISPR tools in manipulating cell death pathways for cancer treatment","","Advancements in CRISPR technology have transformed life sciences and cancer research by enabling precise genome editing and mutation targeting. Cell death pathways are frequently deregulated in cancer cells and are crucial for cancer development and therapy resistance. By identifying and targeting key programmed cell death components, CRISPR systems support screening platforms and genome engineering approaches that can accelerate individualized cancer therapy with minimal toxicity. This study evaluates CRISPR potential for precision treatment while addressing challenges and limitations requiring further research.",{"@graph":14,"@context":73},[15,34,56],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/research-report/","Research & Report",3,{"item":32,"name":10,"@type":21,"position":33},"https://docshare.wps.com/document/lets-make-it-personal-crispr-tools-in-manipulating-cell-death-pathways-for-cancer-treatment/331045/",4,{"url":32,"name":10,"@type":35,"image":36,"author":41,"headline":10,"publisher":44,"fileFormat":47,"inLanguage":8,"description":12,"dateModified":48,"datePublished":49,"encodingFormat":47,"isAccessibleForFree":50,"interactionStatistic":51},"DigitalDocument",{"url":37,"@type":38,"width":39,"height":40},"https://docshare.wps.com/thumbnails/lets-make-it-personal-crispr-tools-in-manipulating-cell-death-pathways-for-cancer-treatment/331045.png","ImageObject",300,407,{"name":42,"@type":43},"Liam","Person",{"url":19,"name":45,"@type":46},"DocShare","Organization","application/pdf","2026-11-01","2026-09-21",true,{"@type":52,"interactionType":53,"userInteractionCount":55},"InteractionCounter",{"@type":54},"ViewAction",5,{"@type":57,"mainEntity":58},"FAQPage",[59,65,69],{"name":60,"@type":61,"acceptedAnswer":62},"How does CRISPR contribute to cancer research according to the document?","Question",{"text":63,"@type":64},"CRISPR is presented as a programmable genome editing and screening tool that helps detect mutations and create precise edits. It supports discovery of relationships between genotype and phenotype relevant to cancer development and therapy resistance.","Answer",{"name":66,"@type":61,"acceptedAnswer":67},"Why are cell death pathways important in cancer treatment?",{"text":68,"@type":64},"The document states that cell death pathways are among the most deregulated processes in cancer cells. These pathways influence cancer development and resistance to therapy, making them critical targets for treatment strategies.",{"name":70,"@type":61,"acceptedAnswer":71},"What role does CRISPR play in personalized cancer therapy?",{"text":72,"@type":64},"Personalized therapy is described as relying on molecular characterization of individual tumors and their microenvironment. The document explains that CRISPR can identify and target specific cell death pathway components and mutations that may drive individualized treatment outcomes.","https://schema.org",{"og:url":32,"og:type":75,"og:title":10,"og:site_name":45,"og:description":12},"article",{"robots":77,"canonical":32},"index,follow",{"doc_id":79,"site_id":7},331045,1790528162,{"code":4,"msg":82,"data":83},"success",[84,88,92,96,100,105,110,114,119,122,126],{"id":22,"doc_module":4,"doc_module_name":25,"category_name":85,"show_sort_weight":86,"slug":87},"Story & Novel",90,"story-novel",{"id":26,"doc_module":4,"doc_module_name":25,"category_name":89,"show_sort_weight":90,"slug":91},"Literature",80,"literature",{"id":33,"doc_module":4,"doc_module_name":25,"category_name":93,"show_sort_weight":94,"slug":95},"Exam",70,"exam",{"id":55,"doc_module":4,"doc_module_name":25,"category_name":97,"show_sort_weight":98,"slug":99},"Comic",60,"comic",{"id":101,"doc_module":4,"doc_module_name":25,"category_name":102,"show_sort_weight":103,"slug":104},6,"Technology",50,"technology",{"id":106,"doc_module":4,"doc_module_name":25,"category_name":107,"show_sort_weight":108,"slug":109},7,"Healthcare",40,"healthcare",{"id":111,"doc_module":4,"doc_module_name":25,"category_name":29,"show_sort_weight":112,"slug":113},8,30,"research-report",{"id":115,"doc_module":4,"doc_module_name":25,"category_name":116,"show_sort_weight":117,"slug":118},9,"Religion & Spirituality",20,"religion-spirituality",{"id":117,"doc_module":4,"doc_module_name":25,"category_name":120,"show_sort_weight":117,"slug":121},"World Cup","world-cup",{"id":123,"doc_module":4,"doc_module_name":25,"category_name":124,"show_sort_weight":123,"slug":125},10,"Lifestyle","lifestyle",{"id":127,"doc_module":4,"doc_module_name":25,"category_name":128,"show_sort_weight":55,"slug":129},19,"General","general",{"code":4,"msg":82,"data":131},{"doc_id":79,"user_id":132,"nickname":42,"user_avatar":133,"doc_module":4,"category_id":111,"category_name":29,"doc_title":10,"doc_description":12,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":55,"is_deleted":4,"is_public":22,"is_downloadable":22,"audit_status":22,"page_count":139,"language":140,"language_code":8,"site_id":7,"html_lang":8,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":12,"update_tm":144,"read_time":145,"preview_page_count":4},8796095461564,"https://ap-avatar.wpscdn.com/davatar_155a257f0dc6eb9ab79c44ca47cae57d","Cell Biol Toxicol (2024) 40:61  \n[https://doi.org/10.1007/s10565-024-09907-z](https://doi.org/10.1007/s10565-024-09907-z)  \nLet’s make it personal: CRISPR tools in manipulating cell death pathways for cancer treatment  \nMobina Bayat · Javid Sadri Nahand  \nReceived: 27 April 2024 / Accepted: 18 July 2024 / Published online: 29 July 2024 © The Author(s) 2024  \nAbstract Advancements in the CRISPR technology, a game-changer in experimental research, have revolutionized various fields of life sciences and more profoundly, cancer research. Cell death pathways are among the most deregulated in cancer cells and are considered as critical aspects in cancer development. Through decades, our knowledge of the mechanisms orchestrating programmed cellular death has increased substantially, attributed to the revolution of cutting-edge technologies. The heroic appearance of CRISPR systems have expanded the available screening platform and genome engineering toolbox to detect mutations and create precise genome edits. In that context, the precise ability of this system for identification and targeting of mutations in cell death signaling pathways that result in cancer development and therapy resistance is an auspicious choice to transform and accelerate the individualized cancer therapy. The concept of personalized cancer therapy stands on the identification of molecular characterization of the individual tumor and its microenvironment in order to provide a precise treatment with the highest possible outcome and minimum toxicity. This study explored the potential of CRISPR technology in precision cancer treatment by identifying and targeting specific cell death pathways. It showed the  \nM. Bayat · J. S. Nahand (*)  \nInfectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz 15731, Iran  \ne-mail: [Javidsadri65@gmail.com](Javidsadri65@gmail.com); [sadrij@tbzmed.ac.ir](sadrij@tbzmed.ac.ir)  \npromise of CRISPR in finding key components and mutations involved in programmed cell death, making it a potential tool for targeted cancer therapy. However, this study also highlighted the challengesand limitations that need to be addressed in future research to fully realize the potential of CRISPR in cancer treatment.  \nKeywords Cancer · Cell death · CRISPR screening · CRISPR-Cas · Personalized therapy  \nIntroduction  \nCRISPR (Clustered Regularly Interspaced Short Palindromic Repeats), once considered as the bacterial immune system for defeating invasive viruses, is now an ineluctable tool in biological studies (Adli 2018) . Jennifer Doudna and Emmanuelle Charpentier, pioneers of revolutionary CRISPR gene editing were the Nobel laureates in chemistry 2020 (Ledford and Callaway 2020) . On December 8th, 2023 the U.S. Food and Drug Administration permitted the first CRISPR treatment for sickle cell disease in patients 12 years and older (Adminstraion USFaD. 2023) . This powerful technology has appeared as a marvelous genome editing tool in cancer research due to its programmability and flexibility (Chow and Chen 2018) . CRISPR screens are a great source of biological discovery that enables to identify the relationship between genotype and phenotype and gene functions in diverse tumor  \nbiologies such as metastasis, cell death, and therapy resistance (Bock et al. 2022a; He et al. 2021) . In fact, in recent years CRISPR screens have been greatly adopted for evaluating cell death pathways and modulators in human cells and various diseases such as Parkinson’s disease, Alzheimer’s disease, and cancer (Pavlou et al. 2023 ; Malireddi et al. 2023 ; Kuehnleet al. 2023) . A genome-wide CRISPR-Cas9 death screen has also been introduced for positive selection and deep sequencing of dying cells (Arroyo et al. 2016) . CRISPR-Cas9 can also be employed for treating c by repairing mutations or knocking out (KO) precise genes. Abundant researches have been carried out on tumor therapy in related fields. Personalized and targeted therapy usi","cbCaitjUhuoDMA2k","https://ap.wps.com/l/cbCaitjUhuoDMA2k","pdf",7597005,31,"English","# Introduction\n## CRISPR as a genome editing tool in biology and cancer research\n## CRISPR screens linking genotype to phenotype\n## Programmed cell death types relevant to cancer\n## Implications for therapeutic targeting and resistance","[{\"question\":\"How does CRISPR contribute to cancer research according to the document?\",\"answer\":\"CRISPR is presented as a programmable genome editing and screening tool that helps detect mutations and create precise edits. It supports discovery of relationships between genotype and phenotype relevant to cancer development and therapy resistance.\"},{\"question\":\"Why are cell death pathways important in cancer treatment?\",\"answer\":\"The document states that cell death pathways are among the most deregulated processes in cancer cells. These pathways influence cancer development and resistance to therapy, making them critical targets for treatment strategies.\"},{\"question\":\"What role does CRISPR play in personalized cancer therapy?\",\"answer\":\"Personalized therapy is described as relying on molecular characterization of individual tumors and their microenvironment. The document explains that CRISPR can identify and target specific cell death pathway components and mutations that may drive individualized treatment outcomes.\"}]","Let’s make it personal: CRISPR tools in manipulating cell death pathways for cancer treatment | PDF",1789991342,78]