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LAMP2A is a limiting protein in molecular chaperone-mediated autophagy, and its role in cisplatin (DDP) resistance in colorectal cancer (CRC) was investigated to inform CRC treatment. Methods assessed LAMP2A expression by qRT-PCR and western blot, then used cell transfection to interfere LAMP2A in CRC/DDP cells, followed by proliferation, migration, invasion, DDP sensitivity, and autophagy analyses using CCK-8, transwell, and western blot. Results showed LAMP2A increased in DDP-resistant CRC and linked to poor prognosis, promoting aggressive behavior and DDP resistance via strengthened autophagy, whereas LAMP2A knockdown reduced aggressiveness and increased DDP sensitivity by restraining autophagy; LAMP2A silencing also curtailed tumor formation and enhanced DDP sensitivity in vivo. Conclusion LAMP2A enhances malignant progression and DDP resistance in CRC/DDP through mediating autophagy, supporting its potential as a therapy biomarker targeting LAMP2A activity.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/lamp2a-regulates-cisplatin-resistance-in-colorectal-cancer-through-mediating-autophagy/342770/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/lamp2a-regulates-cisplatin-resistance-in-colorectal-cancer-through-mediating-autophagy/342770.png","ImageObject",300,407,{"name":92,"@type":93},"Eliana","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-09-23","2026-09-22",true,{"@type":102,"interactionType":103,"userInteractionCount":14},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What is the biological role of LAMP2A investigated in this study?","Question",{"text":112,"@type":113},"LAMP2A is studied as a limiting protein in molecular chaperone-mediated autophagy, and its function is assessed in relation to cisplatin resistance in colorectal cancer.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How did the researchers measure LAMP2A expression and its effects on CRC/DDP cells?",{"text":117,"@type":113},"LAMP2A expression was analyzed using qRT-PCR and western blot. Cells were then transfected to interfere with LAMP2A, and effects were evaluated through assays for proliferation, migration, invasion, DDP sensitivity, and autophagy using methods such as CCK-8, transwell, and western blot.",{"name":119,"@type":110,"acceptedAnswer":120},"What impact does altering LAMP2A have on autophagy and cisplatin resistance?",{"text":121,"@type":113},"Increasing LAMP2A strengthened autophagy and enhanced proliferation, migration, invasion, and DDP resistance, while LAMP2A knockdown or silencing restrained autophagy, reduced aggressiveness, and heightened cellular and in vivo sensitivity to DDP.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},342770,1790167943,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":14,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":41},4398048949847,"https://ap-avatar.wpscdn.com/avatar/400002536579ef2da7f?_k=1778318612642679267","Journal of Cancer Research and Clinical Oncology (2024) 150:242  \n[https://doi.org/10.1007/s00432-024-05775-6](https://doi.org/10.1007/s00432-024-05775-6)  \nLAMP2A regulates cisplatin resistance in colorectal cancer through mediating autophagy  \nZhiliang Shi1 · Shuting Yang1 · Chenglong Shen1 · Jiazhe Shao1 · Fang Zhou1 · Haichen Liu1 · Guoqiang Zhou1,2  \nReceived: 28 January 2024 / Accepted: 3 May 2024 / Published online: 8 May 2024  \n© The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2024  \nAbstract  \nBackground Drug resistance is an important constraint on clinical outcomes in advanced cancers. LAMP2A is a limiting protein in molecular chaperone-mediated autophagy. This study was aimed to explore LAMP2A function in cisplatin (cisdiamminedichloroplatinum, DDP) resistance colorectal cancer (CRC) to seek new ideas for CRC clinical treatment. Methods In this study, LAMP2A expression was analyzed by molecular experimental techniques,such as qRT-PCR and western blot. Then, LAMP2A in cells was interfered by cell transfection experiments. Subsequently, the function of LAMP2Aon proliferation, migration, invasion, DDP sensitivity, and autophagy of CRC/DDP cells were further investigated by a series of experiments, such as CCK-8, transwell, and western blot.  \nResults We revealed that LAMP2A was clearly augmented in DDP-resistant CRC and was related to poor patient prognosis. Functionally, LAMP2A insertion remarkably CRC/DDP proliferation, migration, invasion ability and DDP resistance by strengthen autophagy. In contrast, LAMP2A knockdown limited the proliferation, migration, and invasion while heightened cellular sensitivity to DDP by restraining autophagy in CRC/DDP cells. Furthermore, LAMP2A silencing was able to curb tumor formation and enhance sensitivity to DDP in vivo.  \nConclusion In summary, LAMP2A boosted malignant progression and DDP resistance in CRC/DDP cells through mediating autophagy. Clarifying LAMP2A function in DDP resistance is promising to seek cancer therapies biomarkers targeting LAMP2A activity.  \nKeywords Colorectal cancer · DDP · LAMP2A · Chemoresistance · Autophagy  \nIntroduction  \nGlobally, colorectal cancer (CRC) is a commonly recognized causes of death due to cancer. New CRC cases are going toachieve 3.2 million worldwide by 2040 (Xi and Xu 2021). In recent decades, novel treatments in rapid development have dramatically reduced mortality in cancer patients (Siegel et al. 2021). However, advanced cancers clinical outcomes are still unsatisfactory. The survival rate (within 5 years) of CRC is still only about 50% . This low survival  \n* Guoqiang Zhou [chowgq0568@163.com](chowgq0568@163.com)  \n1 Department of Gastrointestinal Surgery, Affiliated Changshu Hospital to Nantong University, Changshu No. 2 Hospital, Changshu 215500, Jiangsu Province, China  \n2 Department of Gastrointestinal Surgery, Affiliated Changshu Hospital to Nantong University, Changshu No. 2 Hospital, Suzhou 215000, Jiangsu Province, China  \nrate of CRC is highly related to its chemotherapy resistance (Ma et al. 2023). Therefore, elucidating the mechanism of chemoresistance in CRC is a hot research topic at home and abroad. Not to be ignored, acquired chemotherapeutic resistance can arise under therapeutic pressure, which confers acquired epigenetic alterations to cancer cells to enhance survival (Ming et al. 2023) . In addition, the mechanisms that by which emerging resistance occurs are complex, and in addition to the regulation of gene transcription and post-transcriptional modifications, protein degradation has received growing focus for its immediate and fast regulation (Li et al. 2020a). Therefore, it is of great importance to continue to unravel the molecular mechanisms underlying the development of drug resistance in CRC and to seek possible biomarkers for the treatment of CRC drug resistance.  \nAutophagy is considered to be classified into macroautophagy (often referred to as autopha","cbCaifxLas7kNnYU","https://ap.wps.com/l/cbCaifxLas7kNnYU","pdf",5256425,12,"English","# Abstract\n## Background\n## Methods\n## Results\n## Conclusion\n# Introduction","[{\"question\":\"What is the biological role of LAMP2A investigated in this study?\",\"answer\":\"LAMP2A is studied as a limiting protein in molecular chaperone-mediated autophagy, and its function is assessed in relation to cisplatin resistance in colorectal cancer.\"},{\"question\":\"How did the researchers measure LAMP2A expression and its effects on CRC/DDP cells?\",\"answer\":\"LAMP2A expression was analyzed using qRT-PCR and western blot. Cells were then transfected to interfere with LAMP2A, and effects were evaluated through assays for proliferation, migration, invasion, DDP sensitivity, and autophagy using methods such as CCK-8, transwell, and western blot.\"},{\"question\":\"What impact does altering LAMP2A have on autophagy and cisplatin resistance?\",\"answer\":\"Increasing LAMP2A strengthened autophagy and enhanced proliferation, migration, invasion, and DDP resistance, while LAMP2A knockdown or silencing restrained autophagy, reduced aggressiveness, and heightened cellular and in vivo sensitivity to DDP.\"}]","LAMP2A regulates cisplatin resistance in colorectal cancer through mediating autophagy | PDF",1790048154]