[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-340662-105":59,"doc-detail-340662-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","lads-a-powerful-vaccine-platform-for-cancer-immunotherapy-and-prevention","LADS: a powerful vaccine platform for cancer immunotherapy and prevention","","Listeria monocytogenes is explored as an effective intracellular vector for cancer immunotherapy because it delivers tumor antigens to antigen-presenting cells and can trigger strong antitumor immunity. The study introduces Listeria-based Live Attenuated Double Substitution (LADS) featuring two amino acid substitutions in listeriolysin O to achieve near-7000-fold attenuation while preserving immunogenicity. LADS-E7 and LADS-AH1 suppress established tumors, expand tumor-infiltrating antigen-specific CD8+ T cells, modulate immune-response gene expression, drive systemic control of distant growth, and prevent tumor formation.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/lads-a-powerful-vaccine-platform-for-cancer-immunotherapy-and-prevention/340662/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/lads-a-powerful-vaccine-platform-for-cancer-immunotherapy-and-prevention/340662.png","ImageObject",300,407,{"name":92,"@type":93},"Genevieve","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-22","2026-09-22",true,{"@type":102,"interactionType":103,"userInteractionCount":44},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What is the LADS vaccine platform described in the document?","Question",{"text":112,"@type":113},"LADS (Listeria-based Live Attenuated Double Substitution) is a live attenuated vaccine platform engineered by introducing two amino acid substitutions in the virulence factor listeriolysin O while keeping immunogenicity intact.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"Which LADS-based vaccines are developed and what antigens do they deliver?",{"text":117,"@type":113},"Two vaccines are developed: LADS-E7 delivers the HPV type 16 E7 oncoprotein, and LADS-AH1 delivers the murine colon carcinoma immunodominant antigen AH1.",{"name":119,"@type":110,"acceptedAnswer":120},"How do LADS vaccines affect established and distant tumors?",{"text":121,"@type":113},"Treatment with LADS-E7 or LADS-AH1 significantly inhibits and regresses established tumors, increases tumor-infiltrating antigen-specific CD8+ T cells, and induces systemic antitumor responses that help control distant tumor growth.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},340662,1790735347,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":44,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145},1374391974585,"https://ap-avatar.wpscdn.com/davatar_276721f389ce27ea32af1340a28f341c","Sun et al. BMC Biology (2024) 22:291  \n[https://doi.org/10.1186/s12915-024-02086-7](https://doi.org/10.1186/s12915-024-02086-7)  \nBMC Biology  \n RESEARCH Open Access  \nLADS: a powerful vaccine platform for cancer immunotherapy and prevention  \nJing Sun1†, Jing Wang1†, Xin Jiang1†, Jing Xia1, Yue Han1, Mianmian Chen1, Jiali Xu1, Simin Deng1, Changyong Cheng1* and Houhui Song1*  \nAbstract  \nBackground The intracellular bacterium Listeria monocytogenes is an attractive vector for cancer immunotherapy as it can effectively deliver tumor antigens to antigen-presenting cells, leading to a robust antitumor response. Results In this study, we developed a novel vaccine platform called Listeria-based Live Attenuated Double Substitution (LADS), which involves introducing two amino acid substitutions (N478AV479A) into the virulence factor listeriolysin O (LLO) . LADS is a safe vaccine platform, with an attenuation of nearly 7000-fold, while retaining complete immunogenicity due to the absence of deletion of any virulence factors. We developed two LADS-based vaccines, LADS-E7 and LADS-AH1, which deliver the human papillomavirus (HPV) type 16 E7 oncoprotein and murine colon carcinoma immunodominant antigen AH1, respectively. Treatment with LADS-E7 or LADS-AH1 significantly inhibited and regressed established tumors, while also dramatically increasing the populations of tumor-infiltrated antigenspecific CD8+ T cells. RNA-sequencing analysis of tumor tissue samples revealed that LADS-E7 altered the expression of genes related to the immune response. Moreover, intratumoral injection of LADS-based vaccines induced strong antitumor responses, generating systemic antitumor responses to control distant tumor growth. Encouragingly, LADS-E7 or LADS-AH1 immunization effectively prevented tumor formation and growth.  \nConclusions Our findings demonstrate that LADS-based vaccines represent a more powerful platform for the development of immunotherapeutic and preventive vaccines against cancers and infectious diseases.  \nKeywords Aattenuated Listeria monocytogenes, LADS, Tumor immunotherapy, Tumor prevention, Listeriolysin O, Tumor-associated antigen  \n†Jing Sun, Jing Wang and Xin Jiang contributed to this work equally.  \n*Correspondence: Changyong Cheng[lamge@zafu.edu.cn](lamge@zafu.edu.cn)[ ](lamge@zafu.edu.cn)Houhui Song [songhh@zafu.edu.cn](songhh@zafu.edu.cn)  \n1 Key Laboratory of Applied Biotechnology on Animal Science & Veterinary Medicine of Zhejiang Province, Zhejiang Engineering Research Center for Veterinary Diagnostics & Advanced Technology, Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, Belt and Road International Joint Laboratory for One Health and Food Safety, China-Australia Joint Laboratory for Animal Health Big Data Analytics, College of Veterinary Medicine, Zhejiang A&F University, 666 Wusu Street, Lin’an District, Hangzhou, Zhejiang Province 311300, China  \nBackground  \nListeria monocytogenes (LM) is an intracellular foodborne pathogen that can proliferate in a variety of cells, including epithelial cells, fibroblasts, and macrophages [1]. Once the bacterium successfully invades into host cells, it maintains the ability to activate the adaptive immune response via the major histocompatibility complex (MHC) pathways [2]. Those bacteria that do not escape the host cell phagosome elicit an immune response through the MHC II pathway with subsequent activation of CD4+ T cells. However, LM has evolved many sophisticated mechanisms to escape into the cytosols of infected cells mediated by many virulence factors. Listeriolysin O (LLO), a pore-forming cytolysin,  \n© The Author(s) 2024. Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and ","cbCaia8qInoZ9Q2G","https://ap.wps.com/l/cbCaia8qInoZ9Q2G","pdf",5271637,22,"English","# Background\n# LADS vaccine platform development\n## Vaccine design and attenuation\n## Immunogenicity and tumor control\n# Mechanisms and immune response\n## CD8+ T cell activation\n## Tumor-gene expression changes\n# Conclusions","[{\"question\":\"What is the LADS vaccine platform described in the document?\",\"answer\":\"LADS (Listeria-based Live Attenuated Double Substitution) is a live attenuated vaccine platform engineered by introducing two amino acid substitutions in the virulence factor listeriolysin O while keeping immunogenicity intact.\"},{\"question\":\"Which LADS-based vaccines are developed and what antigens do they deliver?\",\"answer\":\"Two vaccines are developed: LADS-E7 delivers the HPV type 16 E7 oncoprotein, and LADS-AH1 delivers the murine colon carcinoma immunodominant antigen AH1.\"},{\"question\":\"How do LADS vaccines affect established and distant tumors?\",\"answer\":\"Treatment with LADS-E7 or LADS-AH1 significantly inhibits and regresses established tumors, increases tumor-infiltrating antigen-specific CD8+ T cells, and induces systemic antitumor responses that help control distant tumor growth.\"}]","LADS: a powerful vaccine platform for cancer immunotherapy and prevention | PDF",1790037988,55]