[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-331993-105":59,"doc-detail-331993-en":131},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":124,"head_meta":126,"extra_data":128,"updated_unix":130},105,"en","knockdown-of-pgbd5-inhibits-the-malignant-progression-of-glioma-through-upregulation-of-the-ppar-pathway","Knockdown of PGBD5 inhibits the malignant progression of glioma through upregulation of the PPAR pathway","","Glioma is the most common primary intracranial malignant tumor and remains associated with poor prognosis due to invasiveness and recurrence. This study examines the biological role of piggyBac transportable element derived 5 (PGBD5) in glioma. Patient-derived glioma and adjacent tissues and multiple cellular and in vivo assays were used to assess PGBD5 expression, migration, invasion, apoptosis, cell-cycle distribution, and downstream molecular pathways. PGBD5 knockdown suppressed malignant phenotypes, including reduced proliferation and tumor growth, and correlated with activation of PPAR signaling.",{"@graph":69,"@context":123},[70,84,106],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/knockdown-of-pgbd5-inhibits-the-malignant-progression-of-glioma-through-upregulation-of-the-ppar-pathway/331993/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/knockdown-of-pgbd5-inhibits-the-malignant-progression-of-glioma-through-upregulation-of-the-ppar-pathway/331993.png","ImageObject",300,407,{"name":92,"@type":93},"Ava Thompson","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-23","2026-09-21",true,{"@type":102,"interactionType":103,"userInteractionCount":105},"InteractionCounter",{"@type":104},"ViewAction",13,{"@type":107,"mainEntity":108},"FAQPage",[109,115,119],{"name":110,"@type":111,"acceptedAnswer":112},"What is the main focus of this study?","Question",{"text":113,"@type":114},"The study investigates how PGBD5 knockdown affects glioma malignant progression, particularly through the PPAR signaling pathway.","Answer",{"name":116,"@type":111,"acceptedAnswer":117},"How were PGBD5 expression levels measured?",{"text":118,"@type":114},"PGBD5 mRNA and protein expression were assessed using reverse transcription-quantitative PCR and western blotting.",{"name":120,"@type":111,"acceptedAnswer":121},"What cellular effects occur after PGBD5 knockdown?",{"text":122,"@type":114},"PGBD5 knockdown inhibits glioma cell migration and invasion, promotes apoptosis, and causes cell-cycle arrest at the G2/M phase.","https://schema.org",{"og:url":83,"og:type":125,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":127,"canonical":83},"index,follow",{"doc_id":129,"site_id":62},331993,1790415815,{"code":4,"msg":5,"data":132},{"doc_id":129,"user_id":133,"nickname":92,"user_avatar":134,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":135,"file_id":136,"file_url":137,"file_type":138,"file_size":139,"view_count":105,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":140,"language":141,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":142,"faqs":143,"seo_title":144,"seo_description":67,"update_tm":145,"read_time":146,"preview_page_count":4},1649267921044,"https://us-avatar.wpscdn.com/avatar/1800007509477c92dfb?_k=1786009248482753345","INTERNATIONAL JOURNAL OF ONCOLOGY 64: 55, 2024  \nKnockdown of PGBD5 inhibits the malignant progression of glioma through upregulation of the PPAR pathway  \nPENGREN LUO 1-3* , JINHONG YANG3* , LIPENG JIAN 1 , JIGEN DONG 1 , SHI YIN 1 , CHAO LUO 1 and SHUAI ZHOU 1-3  \n1Department of Neurosurgery, The First People's Hospital of Yunnan Province; 2Department of Neurosurgery, The Affiliated Hospital of Kunming University of Science and Technology; 3Medical School, Kunming University of Science and Technology, Kunming, Yunnan 650500, P.R. China  \nReceived November 1, 2023; Accepted February 5, 2024  \nDOI: 10.3892/ijo.2024.5643  \nAbstract. Glioma is the most common type of primary intracranial malignant tumor, and because of its high invasiveness and recurrence, its prognosis remains poor. The present study investigated the biological function of piggyBac transportable element derived 5 (PGBD5) in glioma . Glioma and para-cancerous tissues were obtained from five patients . Reverse transcription-quantitative PCRand western blotting were used to detect the expression levels of PGBD5 . Transwell assay and flow cytometry were used to evaluate cell migration, invasion, apoptosis and cell cycle distribution . In addition, a nude mouse tumor transplantation model was established to study the downstream pathways of PGBD5 and the molecular mechanism was analyzed using transcriptome sequencing. The mRNA and protein expression levels of PGBD5 were increased in glioma tissues and cells . Notably, knockdown of PGBD5 in vitro could inhibit the migration and invasion of glioma cells . In addition, the knockdown of PGBD5 expression promoted apoptosis and caused cell cycle arrest in the G2/M phase, thus inhibiting cell proliferation. Furthermore, in vivo experiments revealed that knockdown of PGBD5 expression could inhibit Ki67 expression and slow tumor growth. Changes in PGBD5 expression were also shown tobe closely related to the peroxisome proliferator-activated receptor (PPAR) signaling pathway. In conclusion, interference with PGBD5 could inhibit the malignant progression of glioma through the PPAR pathway, suggesting that PGBD5 may be a potential molecular target of glioma .  \nCorrespondence to: Dr Shuai Zhou, Department of Neurosurgery, The First People's Hospital of Yunnan Province, 157 Jinbi Road, Kunming, Yunnan 650500, P.R. China  \nE-mail: [zshuai@kust.edu.cn](zshuai@kust.edu.cn)  \n*Contributed equally  \nKey words: glioma, PGBD5, tumor growth, PPAR signaling, transposons  \nIntroduction  \nGlioma is the most common type of primary malignant tumor of the central nervous system worldwide. Glioma is associated with high rates of disability and mortality, and a poor prognosis (1) . According to the World Health Organization (WHO) pathological classification, grades I and II represent low-grade glioma (LGG), whereas grades III and IV represent high-grade glioma (HGG) (1) . Among all glioma cases, glioblastoma (GBM) accounts for ~57% in the US, and its median survival time is \u003C2 years (2) . Although the survival duration of patients with LGG is longer than HGG, this type of tumor may eventually progress to HGG after multiple relapses (3,4) . At present, the preferred treatment for glioma remains surgical resection with maximum safety, with temozolomide chemotherapy and radiotherapy as auxiliary methods (5) . However, owing to characteristics such as drug resistance, radiotherapy resistance and frequent recurrence of glioma, therapeutic efficacy remains poor (6,7) . Therefore, identifying effective molecular targets (8-11) that help navigate this issue in glioma treatment is necessary.  \nTransposons or transposable elements (TEs) are present in almost all organisms; these mobile genetic elements account for~50% of the human genome (12,13) . Transposons are usually classified as RNA-based retrotransposons and ‘cut-paste’ or‘cut-copy’ types of transposons (14) . Transposons not only serve an active role in the regulation of gene expression, but can o","cbCaifN7mAVaTykQ","https://ap.wps.com/l/cbCaifN7mAVaTykQ","pdf",4836456,14,"English","# Introduction\n## Transposons and TE regulation\n## PiggyBac and PGBD5 background\n# Abstract","[{\"question\":\"What is the main focus of this study?\",\"answer\":\"The study investigates how PGBD5 knockdown affects glioma malignant progression, particularly through the PPAR signaling pathway.\"},{\"question\":\"How were PGBD5 expression levels measured?\",\"answer\":\"PGBD5 mRNA and protein expression were assessed using reverse transcription-quantitative PCR and western blotting.\"},{\"question\":\"What cellular effects occur after PGBD5 knockdown?\",\"answer\":\"PGBD5 knockdown inhibits glioma cell migration and invasion, promotes apoptosis, and causes cell-cycle arrest at the G2/M phase.\"}]","Knockdown of PGBD5 inhibits the malignant progression of glioma through upregulation of the PPAR pathway | PDF",1789996206,35]