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Immunohistochemistry and Western blot assessed NCAPD3 expression, while CCK-8, EdU, Transwell, and scratch assays evaluated proliferation, invasion, and migration. Flow cytometry measured cell cycle and apoptosis; RNA-seq and rescue experiments probed mechanisms. NCAPD3 was elevated in NSCLC and associated with poorer prognosis.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/knockdown-of-ncapd3-inhibits-the-tumorigenesis-of-non-small-cell-lung-cancer-by-regulation-of-the-pi3kakt-pathway/333276/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/knockdown-of-ncapd3-inhibits-the-tumorigenesis-of-non-small-cell-lung-cancer-by-regulation-of-the-pi3kakt-pathway/333276.png","ImageObject",300,407,{"name":92,"@type":93},"Lucas Martin","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-29","2026-09-21",true,{"@type":102,"interactionType":103,"userInteractionCount":34},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What is the study investigating about NCAPD3 in NSCLC?","Question",{"text":112,"@type":113},"The study examines whether NCAPD3 knockdown suppresses NSCLC tumorigenesis and clarifies the underlying molecular mechanism involved in disease progression.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How did the researchers measure NCAPD3 effects on NSCLC cells?",{"text":117,"@type":113},"They used immunohistochemistry and Western blot for NCAPD3 expression, CCK-8 and EdU for proliferation, Transwell and scratch assays for invasion and migration, and flow cytometry for cell cycle and apoptosis.",{"name":119,"@type":110,"acceptedAnswer":120},"What pathway did the study identify as critical for NCAPD3-driven effects?",{"text":121,"@type":113},"RNA-sequencing and rescue experiments indicated that NCAPD3 contributes to NSCLC carcinogenesis by regulating the PI3K/Akt/FOXO4 pathway, and IGF-1 could reverse NCAPD3 knockdown effects.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},333276,1790179443,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":34,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":41,"preview_page_count":4},8796095360427,"https://ap-avatar.wpscdn.com/davatar_994ba38a5ba835b3df7d355c54d3ed8d","Yang et al. BMC Cancer (2024) 24:408 BMC Cancer  \n[https://doi.org/10.1186/s12885-024-12131-x](https://doi.org/10.1186/s12885-024-12131-x)  \nRESEARCH Open Access  \nKnockdown of NCAPD3 inhibits the tumorigenesis of non-small cell lung cancer by regulation of the PI3K/Akt pathway  \nFan Yang 1, Yunfeng Zheng2, Qiong Luo3, Suyun Zhang4*, Sheng Yang3* and Xiangqi Chen 1*  \nAbstract  \nBackground Accumulating evidence indicates that aberrant non-SMC condensin II complex subunit D3 (NCAPD3) is associated with carcinogenesis of various cancers. Nevertheless, the biological role of NCAPD3 in the pathogenesis of non-small cell lung cancer (NSCLC) remains unclear.  \nMethods Immunohistochemistry and Western blot were performed to assess NCAPD3 expression in NSCLC tissues and cell lines. The ability of cell proliferation, invasion, and migration was evaluated by CCK-8 assays, EdU assays, Transwell assays, and scratch wound healing assays. Flow cytometry was performed to verify the cell cycle and apoptosis. RNA-sequence and rescue experiment were performed to reveal the underlying mechanisms.  \nResults The results showed that the expression of NCAPD3 was significantly elevated in NSCLC tissues. High NCAPD3 expression in NSCLC patients was substantially associated with a worse prognosis. Functionally, knockdown of NCAPD3 resulted in cell apoptosis and cell cycle arrest in NSCLC cells as well as a significant inhibition of proliferation, invasion, and migration. Furthermore, RNA-sequencing analysis suggested that NCAPD3 contributes to NSCLC carcinogenesis by regulating PI3K/Akt/FOXO4 pathway. Insulin-like growth factors-1 (IGF-1), an activator of PI3K/Akt signaling pathway, could reverse NCAPD3 silence-mediated proliferation inhibition and apoptosis in NSCLC cells. Conclusion NCAPD3 suppresses apoptosis and promotes cell proliferation via the PI3K/Akt/FOXO4 signaling pathway, suggesting a potential use for NCAPD3 inhibitors as NSCLC therapeutics.  \nKeywords NCAPD3, Non-small cell lung cancer, PI3K/Akt/FOXO4, Proliferation, Apoptosis  \n*Correspondence: Suyun Zhang [zqzzsy@126.com](zqzzsy@126.com)[ ](zqzzsy@126.com)Sheng Yang [dryangxh@126.com](dryangxh@126.com)[ ](dryangxh@126.com)Xiangqi Chen [drchxqtg@126.com](drchxqtg@126.com)  \n1Department of Respiratory Medicine, Fujian Medical University Union Hospital, NO.29 of Xinquan Road, Gulou District, 350000 Fuzhou City, Fujian Province, China  \n2Department of Gastric Surgery, Fujian Medical University Union Hospital, NO.29 of Xinquan Road, Gulou District, 350000 Fuzhou City, Fujian Province, China  \n3Department of Oncology, Fujian Medical University Union Hospital, NO.29 of Xinquan Road, Gulou District, 350000 Fuzhou City, Fujian Province, China  \n4Department of Internal Medicine, Fujian Medical University Union Hospital, 350001 Fuzhou, Fujian, China  \n© The Author(s) 2024. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit [http://creativecommons.org/l](http://creativecommons.org/l)icenses/by/4.0/. The Creative Commons Public Domain Dedication waiver ([http://creativecommons.org/publicdomain/zero/1.0/](http://creativecommons.org/publicdomain/zero/1.0/)) applies to the data made available in this article, unless otherwise stated in a credit line to the data","cbCaiqi8ta0amW3V","https://ap.wps.com/l/cbCaiqi8ta0amW3V","pdf",10035589,12,"English","# Introduction\n## Background and clinical context\n## Role of NCAPD3 and knowledge gap\n# Abstract\n## Background\n## Methods\n## Results\n## Conclusion","[{\"question\":\"What is the study investigating about NCAPD3 in NSCLC?\",\"answer\":\"The study examines whether NCAPD3 knockdown suppresses NSCLC tumorigenesis and clarifies the underlying molecular mechanism involved in disease progression.\"},{\"question\":\"How did the researchers measure NCAPD3 effects on NSCLC cells?\",\"answer\":\"They used immunohistochemistry and Western blot for NCAPD3 expression, CCK-8 and EdU for proliferation, Transwell and scratch assays for invasion and migration, and flow cytometry for cell cycle and apoptosis.\"},{\"question\":\"What pathway did the study identify as critical for NCAPD3-driven effects?\",\"answer\":\"RNA-sequencing and rescue experiments indicated that NCAPD3 contributes to NSCLC carcinogenesis by regulating the PI3K/Akt/FOXO4 pathway, and IGF-1 could reverse NCAPD3 knockdown effects.\"}]","Knockdown of NCAPD3 inhibits the tumorigenesis of non-small cell lung cancer by regulation of the PI3K/Akt pathway | PDF",1790001768]