[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"doc-detail-150008-en":3,"doc-seo-150008-105":30,"detail-sidebar-cat-0-en-105":90},{"code":4,"msg":5,"data":6},0,"success",{"doc_id":7,"user_id":8,"nickname":9,"user_avatar":10,"doc_module":4,"category_id":11,"category_name":12,"doc_title":13,"doc_description":14,"doc_content":15,"file_id":16,"file_url":17,"file_type":18,"file_size":19,"view_count":4,"is_deleted":4,"is_public":20,"is_downloadable":20,"audit_status":20,"page_count":21,"language":22,"language_code":23,"site_id":24,"html_lang":23,"table_of_contents":25,"faqs":26,"seo_title":27,"seo_description":14,"update_tm":28,"read_time":29},150008,8796093062539,"8796093062539","",7,"Healthcare","Kelp Cultivation Handbook - Step-by-step Method","Kelp Cultivation Handbook compiles practical resources and a step-by-step nursery process used by Puget Sound Restoration Fund (PSRF) to cultivate native Puget Sound kelp for later outplanting. It focuses on planning a compliant cultivation space, selecting equipment and spools, preparing sterile seawater, and managing temperature, light, aeration, pH, and nutrient cycling. The handbook also provides a cultivation calendar, spool preparation and lab logging procedures, contamination prevention guidance, and appendix resources for culture materials, costs, conditions, nutrient media, maintenance checklists, and a kelp-focused glossary.","PUGET SOUND  \nRESTORATION FUND  \n# Kelp Cultivation Handbook\n\nThis handbook is a compilation of helpful resources and the step-by-step process staff at PugetSound Restoration Fund (PSRF)have used (with success!)to cultivate native Puget Sound kelp ina nursery for the purpose of outplanting.This process has been primarily used for sugar kelp(Saccharina latissima),particularly in New England and Europe,but PSRF has used it extensivelyfor bull kelp(Nereocystis luetkeana),and it can be applied to most kelp species of interest.  \n## Table of Contents\n\n1.READ THIS FIRST'-Major Considerations at the onset of cultivating kelp+other PSRFpg.2lessons learned  \nII.  Kelp Cultivation Calendar for Puget Soundpg.3  \nⅢI.  Materials/Supplies Checklist  \npg.4  \nIV.  Spool preparation procedure and lab logpg.5  \nV.  PSRF's Step-by-step Method for Cultivating Kelp(up to the outplanting stage)  \npg.7  \nAppendices/Useful Resources  \nNursery Set-up Recommendations (from Ocean Approved)  \nGeneral Culture System Materials and Estimated Costs(from Connecticut Sea Grant)  \nOptimal Kelp Culture Conditions(from Connecticut Sea Grant)  \nNutrient Media Suggestions and Supplies Checklist(from Connecticut Sea Grant and OceanApproved)  \nStep-by-step Cheat-sheet (from Ocean Approved)  \nCounting Zoospores &Calculating Stocking Density-Explanation and Worksheet(from OceanApproved)  \nNursery Daily Maintenance Checklist,Management of Environmental Parameters,and NurseryTasks(from Ocean Approved)  \nGlossary (from Ocean Approved)  \nSocio-ecological considerations for Puget Sound kelp species (from Buckner et.al.,in review)  \n## READ THIS FIRST\n\nMajor Considerations and questions to ask yourself at the onset of kelp cultivation:  \n1.Are you familiar with the rules,regulations and permits required by Washington state tocultivate kelp/seaweed,including sourcing seed?  \n2.What are you setting this nursery up for?What will be the scale of cultivation?  \n3.In setting up your cultivation space/nursery how do you plan to maintain the following?  \na.Temperature control (cold room or chilled water via recirculation or heatexchange)  \nb.Light supply &control (irradiance,wavelength,photoperiod)  \nc.Aeration (maintain pH,nutrient cycling)  \nd.Sterile seawater production and storage  \n4.When selecting aquaria,first build a spool for your farm application,determine thenumber of spools you willbe seeding (think back to question 2)and then source aquariato accommodate those spool dimensions.  \nLessons learned by PSRF(aka where are the 'tricky'spots in the process?):  \n● Bull kelp sorus can be collected from rocky shore habitats(ex.Strait of Juan de Fuca)year-round in most years where the adults can overwinter.  \n● February is the best time to transfer cultivated bull kelp seed,if making benthicdeployments.  \n● Sorus collected from 1 plant is sufficient to produce spore stock,however collectingsorus from 10-20 plants is recommended to attempt to capture the genetic diversitywithin wild populations.  \n● In designing the layout of your cultivation space,ensure proper and efficient workflowvia easy access for cleaning tanks and moving materials/equipment around space(ex.inserting/removing spools).  \n● Spool preparation is a crucial step-see page 5.  \n● Maximize spool number to media volume (little extra space)in cultivation aquaria,to beefficient with media.  \n● See Table 2.1 in Ocean Approved 'Nursery Recommendations'in Appendices.Note:PSRFuses a combination of filtration and UV treatment to produce the large volumes ofsterile water.  \n● Buy nutrient media kits(as opposed to making your own,though to do so check out'Nutrient Media Suggestions and Supplies Checklist'from Connecticut Sea Grant andOcean Approved).PSRF recommends PES or Guillard's F/2.  \no Change media every 10-14 days unless contamination is occurring  \no Provide nutrient media with 10mL media stock/L sterile seawater  \n● Batch sporulation-see the note in Step 9 of 'Sorus prep &desiccation.  \n● We do not recommend sporophy","cbCailNP51bsdbII","https://ap.wps.com/l/cbCailNP51bsdbII","pdf",11771443,1,78,"English","en",105,"# READ THIS FIRST\n## Major Considerations at the onset of kelp cultivation\n## Lessons learned by PSRF\n# Kelp Cultivation Calendar for Puget Sound\n## Before Starting\n## Day 0 or 1 onward","[{\"question\":\"What major considerations and permits should be checked before starting kelp cultivation in Washington state?\",\"answer\":\"The handbook emphasizes understanding Washington state rules, regulations, permits, and sourcing requirements for kelp/seaweed seed before beginning cultivation.\"},{\"question\":\"How does PSRF handle nursery environmental parameters during kelp cultivation?\",\"answer\":\"The process includes temperature control, managed light supply and control (irradiance, wavelength, photoperiod), aeration to maintain pH and support nutrient cycling, and production/storage of sterile seawater.\"},{\"question\":\"What are the key lessons PSRF highlights to avoid difficulties during the cultivation workflow?\",\"answer\":\"PSRF notes critical steps such as proper spool preparation, maximizing spool number relative to media volume, using filtration plus UV for sterile water, buying recommended nutrient media kits, and minimizing contamination sources including spore stock, people, media, and the aeration system.\"}]","Kelp Cultivation Handbook - Step-by-step Method | PDF",1787811728,197,{"code":4,"msg":31,"data":32},"ok",{"site_id":24,"language":23,"slug":33,"title":13,"keywords":10,"description":14,"schema_data":34,"social_meta":85,"head_meta":87,"extra_data":89,"updated_unix":28},"kelp-cultivation-handbook-step-by-step-method",{"@graph":35,"@context":84},[36,53,67],{"@type":37,"itemListElement":38},"BreadcrumbList",[39,43,47,50],{"item":40,"name":41,"@type":42,"position":20},"https://docshare.wps.com","Home","ListItem",{"item":44,"name":45,"@type":42,"position":46},"https://docshare.wps.com/document/","Document",2,{"item":48,"name":12,"@type":42,"position":49},"https://docshare.wps.com/document/healthcare/",3,{"item":51,"name":13,"@type":42,"position":52},"https://docshare.wps.com/document/kelp-cultivation-handbook-step-by-step-method/150008/",4,{"url":51,"name":13,"@type":54,"author":55,"headline":13,"publisher":57,"fileFormat":60,"inLanguage":23,"description":14,"dateModified":61,"datePublished":61,"encodingFormat":60,"isAccessibleForFree":62,"interactionStatistic":63},"DigitalDocument",{"name":9,"@type":56},"Person",{"url":40,"name":58,"@type":59},"DocShare","Organization","application/pdf","2026-08-27",true,{"@type":64,"interactionType":65,"userInteractionCount":4},"InteractionCounter",{"@type":66},"ViewAction",{"@type":68,"mainEntity":69},"FAQPage",[70,76,80],{"name":71,"@type":72,"acceptedAnswer":73},"What major considerations and permits should be checked before starting kelp cultivation in Washington state?","Question",{"text":74,"@type":75},"The handbook emphasizes understanding Washington state rules, regulations, permits, and sourcing requirements for kelp/seaweed seed before beginning cultivation.","Answer",{"name":77,"@type":72,"acceptedAnswer":78},"How does PSRF handle nursery environmental parameters during kelp cultivation?",{"text":79,"@type":75},"The process includes temperature control, managed light supply and control (irradiance, wavelength, photoperiod), aeration to maintain pH and support nutrient cycling, and production/storage of sterile seawater.",{"name":81,"@type":72,"acceptedAnswer":82},"What are the key lessons PSRF highlights to avoid difficulties during the cultivation workflow?",{"text":83,"@type":75},"PSRF notes critical steps such as proper spool preparation, maximizing spool number relative to media volume, using filtration plus UV for sterile water, buying recommended nutrient media kits, and minimizing contamination sources including spore stock, people, media, and the aeration system.","https://schema.org",{"og:url":51,"og:type":86,"og:title":13,"og:site_name":58,"og:description":14},"article",{"robots":88,"canonical":51},"index,follow",{"doc_id":7,"site_id":24},{"code":4,"msg":5,"data":91},[92,96,100,104,109,114,117,122,127,130,134],{"id":20,"doc_module":4,"doc_module_name":45,"category_name":93,"show_sort_weight":94,"slug":95},"Story & Novel",90,"story-novel",{"id":46,"doc_module":4,"doc_module_name":45,"category_name":97,"show_sort_weight":98,"slug":99},"Literature",80,"literature",{"id":52,"doc_module":4,"doc_module_name":45,"category_name":101,"show_sort_weight":102,"slug":103},"Exam",70,"exam",{"id":105,"doc_module":4,"doc_module_name":45,"category_name":106,"show_sort_weight":107,"slug":108},5,"Comic",60,"comic",{"id":110,"doc_module":4,"doc_module_name":45,"category_name":111,"show_sort_weight":112,"slug":113},6,"Technology",50,"technology",{"id":11,"doc_module":4,"doc_module_name":45,"category_name":12,"show_sort_weight":115,"slug":116},40,"healthcare",{"id":118,"doc_module":4,"doc_module_name":45,"category_name":119,"show_sort_weight":120,"slug":121},8,"Research & Report",30,"research-report",{"id":123,"doc_module":4,"doc_module_name":45,"category_name":124,"show_sort_weight":125,"slug":126},9,"Religion & Spirituality",20,"religion-spirituality",{"id":125,"doc_module":4,"doc_module_name":45,"category_name":128,"show_sort_weight":125,"slug":129},"World Cup","world-cup",{"id":131,"doc_module":4,"doc_module_name":45,"category_name":132,"show_sort_weight":131,"slug":133},10,"Lifestyle","lifestyle",{"id":135,"doc_module":4,"doc_module_name":45,"category_name":136,"show_sort_weight":105,"slug":137},19,"General","general"]