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The sheet covers purification and size selection of single- and double-stranded DNA using paramagnetic beads in a PEG/NaCl-optimized buffer, including shipment and storage conditions, handling requirements, disposal as biohazardous waste, and quality control expectations. It further describes critical factors affecting DNA recovery, fragment-size distribution, and compatibility constraints for validated workflows.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":30,"@type":76,"position":81},"https://docshare.wps.com/document/technology/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/kapa-hyperpure-beads-kr1705-v1-19-technical-data-sheet/670801/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/kapa-hyperpure-beads-kr1705-v1-19-technical-data-sheet/670801.png","ImageObject",300,407,{"name":92,"@type":93},"jay","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-11-02","2026-11-01",true,{"@type":102,"interactionType":103,"userInteractionCount":8},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What is KAPA HyperPure Beads KR1705 used for in NGS workflows?","Question",{"text":112,"@type":113},"It is used for fast purification or size selection of single- and double-stranded DNA in next-generation sequencing workflows, supporting inputs of 1 ng to 5 µg in a single reaction.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How should the beads be shipped and stored to maintain functionality?",{"text":117,"@type":113},"Store the product at 2°C to 8°C after receipt and protect it from light when not in use. Do not store at -15°C to -25°C, and it may be shipped on dry ice or ice packs depending on destination.",{"name":119,"@type":110,"acceptedAnswer":120},"What factors most affect DNA recovery and fragment size distribution?",{"text":121,"@type":113},"Binding temperature, complete resuspension/homogeneity before aspirating, the volumetric ratio of beads to sample, and thorough mixing of DNA and beads prior to binding are critical for optimal performance.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},670801,1793571780,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":29,"category_name":30,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":8,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":129,"read_time":144},3985747858866,"https://ap-avatar.wpscdn.com/davatar_a8503ba1806abce46bf441b54a3ca4cd","Technical Data Sheet  \nKAPA HyperPure Beads KR1705 – v1 .19  \nThis Technical Data Sheet provides product information and detailed protocols for the implementation of KAPA HyperPure Beads in NGS workflows.  \nThis document applies to KAPA HyperPure Beads 08963835001, 08963843001, 08963851001, 08963860001 and 08963878001.  \nContents  \nProduct Description ............................ 2  \nProduct Specifications .......................... 2  \nShipping and Storage ........................ 2  \nHandling ................................... 2  \nDisposal ................................... 2  \nQuality Control .............................. 2  \nImportant Parameters ........................... 2  \nFactors that Impact Overall Performance ......... 2  \nDNA Purification (Cleanup) ..................... 3  \nSize Selection in NGS Workflows................ 3  \nCompatibility with KAPA Library Preparation Kits ..... 4  \nProtocols ..................................... 5  \nGenomic DNA Purification (Cleanup) ............. 5  \nNotes on RNA Purification (Cleanup) ............. 5  \nCleanup of Fragmented DNA in NGS Workflows.... 6  \nSize Selection in NGS Workflows................ 8  \nAppendix – Troubleshooting ..................... 10  \nRestrictions and Liabilities....................... 11  \nNote to Purchaser: Limited Product Warranty ....... 11  \nNote to Purchaser: Limited License ............... 11  \n\n| Kapa/Roche Kit Codes and Components |  |\n| --- | --- |\n| KK8007\u003Cbr>08963835001 | KAPA HyperPure Beads 5 mL |\n| KK8008\u003Cbr>08963843001 | KAPA HyperPure Beads 30 mL |\n| KK8009\u003Cbr>08963851001 | KAPA HyperPure Beads 60 mL |\n| KK8010\u003Cbr>08963860001 | KAPA HyperPure Beads 450 mL |\n| KK8011\u003Cbr>08963878001 | KAPA HyperPure Beads 4 x 60 mL |\n\n\n| Quick Notes |\n| --- |\n| • KAPA HyperPure Beads is designed for the rapid and efficient purification of DNA, for inputs ranging from 1 ng – 5 µg in a single reaction.\u003Cbr>• KAPA HyperPure Beads is NOT compatible with KAPA library preparation kits that implement a“with-bead” protocol. Please refer to the section Compatibility with KAPA Library Preparation Kits (p. 4) for validated workflows.\u003Cbr>• KAPA HyperPure Beads are compatible with manual processing and automated liquid handling.\u003Cbr>• KAPA HyperPure Beads may be shipped on dry ice which does not affect product performance. Upon receipt the product must be stored at 2ºC to 8ºC, and protected from light when not in use.\u003Cbr>• For optimal performance, always ensure that the KAPA HyperPure Beads is equilibrated to room temperature, that the beads are fully resuspended before use, and that the DNA and bead solutions are thoroughly mixed prior to the binding incubation.\u003Cbr>• Several factors may impact the efficiency of DNA recovery and exclusion. Please refer to the Important Parameters section for details. |\n\nEffective date: August 2019 For Research Use Only. Not for use in diagnostic procedures.  \nProduct Description  \nKAPA HyperPure Beads is a suspension of paramagnetic beads in a buffer optimized for the purification or size selection of single- and double-stranded DNA in nextgeneration sequencing and other molecular biology workflows. The product is designed for fast and reliable purification or size selection of 1 ng to 5 µg DNA in a single reaction. KAPA HyperPure Beads is compatible with manual processing or automated liquid handling and enables efficient recovery of input DNA in both formats.  \nFor purification and size selection of DNA, the KAPA HyperPure Beads buffer includes an optimized concentration of PEG/NaCl—a crowding agent designed to drive DNA molecules to the beads for binding. The volumetric ratio of KAPA HyperPure Beads to sample is the critical factor in determining the size distribution of DNA fragments retained by the beads. The volume (ratio) maybe modified/optimized based upon the specific application and/or point in the library construction workflow where a cleanup or size selection is employed.  \nProduct Specifications Shipping and Storage  \nKAPA H","cbCaivpaJwZwsxFb","https://ap.wps.com/l/cbCaivpaJwZwsxFb","pdf",769209,11,"English","# Product Description\n## Product Specifications\n## Shipping and Storage\n## Handling\n## Disposal\n## Quality Control\n## Important Parameters\n## Factors that Impact Overall Performance\n## DNA Purification (Cleanup)\n## Size Selection in NGS Workflows\n## Compatibility with KAPA Library Preparation Kits\n## Protocols\n## Genomic DNA Purification (Cleanup)\n## Notes on RNA Purification (Cleanup)\n## Cleanup of Fragmented DNA in NGS Workflows\n## Size Selection in NGS Workflows\n# Appendix – Troubleshooting\n# Restrictions and Liabilities\n# Note to Purchaser: Limited Product Warranty\n# Note to Purchaser: Limited License","[{\"question\":\"What is KAPA HyperPure Beads KR1705 used for in NGS workflows?\",\"answer\":\"It is used for fast purification or size selection of single- and double-stranded DNA in next-generation sequencing workflows, supporting inputs of 1 ng to 5 µg in a single reaction.\"},{\"question\":\"How should the beads be shipped and stored to maintain functionality?\",\"answer\":\"Store the product at 2°C to 8°C after receipt and protect it from light when not in use. Do not store at -15°C to -25°C, and it may be shipped on dry ice or ice packs depending on destination.\"},{\"question\":\"What factors most affect DNA recovery and fragment size distribution?\",\"answer\":\"Binding temperature, complete resuspension/homogeneity before aspirating, the volumetric ratio of beads to sample, and thorough mixing of DNA and beads prior to binding are critical for optimal performance.\"}]","KAPA HyperPure Beads KR1705 - v1 .19 - Technical Data Sheet | PDF",28]