[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"doc-detail-167207-en":3,"doc-seo-167207-105":30,"detail-sidebar-cat-0-en-105":92},{"code":4,"msg":5,"data":6},0,"success",{"doc_id":7,"user_id":8,"nickname":9,"user_avatar":10,"doc_module":4,"category_id":11,"category_name":12,"doc_title":13,"doc_description":14,"doc_content":15,"file_id":16,"file_url":17,"file_type":18,"file_size":19,"view_count":20,"is_deleted":4,"is_public":20,"is_downloadable":20,"audit_status":20,"page_count":21,"language":22,"language_code":23,"site_id":24,"html_lang":23,"table_of_contents":25,"faqs":26,"seo_title":27,"seo_description":14,"update_tm":28,"read_time":29},167207,549758146520,"Patrick","https://ap-avatar.wpscdn.com/avatar/80002397d8c0411e94?_k=1775819394049821470",8,"Research & Report","Isolation and target identification of antihepatoma polyprenylphenols from the edible mushroom Suillus granulatus","Eight polyprenylphenol derivatives were isolated from the wild edible mushroom Suillus granulatus, including seven novel compounds named suillin F–L (2–8) and one previously reported compound (1). HR-ESI-MS together with 1D and 2D NMR supported structure elucidation, while compound 8 absolute configuration was determined by comparison of experimental and calculated ECD data. All isolates were tested for cytotoxicity against HepG2 liver cancer cells, and compounds 1 and 3–6 showed significant antitumor activity with IC50 values of 8.19–13.97 μM. DARTS and LC-MS/MS identified HSP90AA1 as the direct target of compound 5, confirmed by molecular docking.","Food & Function  \nPAPER  \nPublished on 07 June 2024 Licence and permissions  \nCite this: Food Funct., 2024, 15, 7430  \nReceived 2nd April 2024, Accepted 6th June 2024  \nDOI: 10.1039/d4fo01500b[rsc.li/food-function](rsc.li/food-function)  \nIsolation and target identification of antihepatoma polyprenylphenols from the edible mushroom Suillus granulatus†  \nHanyu Zhao,a Miaomiao Xiong,a Xiaomin Yang,a Lan Yao,b Zhuang Li,a Jinxiu Zhang*a and Jianhua Lv  *a  \nEight polyprenylphenol derivatives were isolated from the wild edible mushroom Suillus granulatus, including seven novel compounds, named suillin F–L (2–8), and one previously identified compound (1) . The structures of the new compounds were elucidated using HR-ESI-MS and 1D and 2D NMR data. The absolute configuration of compound 8 was assigned based on the comparison of the experimental and calculated ECD data. All isolated compounds were evaluated for their cytotoxicity against HepG2 cancer cell lines. Compounds 1 and 3–6 demonstrated significant antitumor activity compared to the positive control (cisplatin), with IC50 values ranging from 8 . 19 to 13 .97 μM. Furthermore, DARTS assay and LC-MS/ MS analysis were used to identify HSP90AA1 as the direct target of compound 5, and the interaction between compound 5 and HSP90AA1 was verified by molecular docking.  \n1. Introduction  \nMushrooms refer to macrofungi possessing discernible fruiting bodies that are visible to the unaided eye.1,2 Wild edible mushrooms have long been a significant component of various cultural diets such as the American, European, and Asian.3,4 Moreover, mushrooms have been utilized as fundamental components in folklore medicine and ethno-pharmacology due to their various health-promoting properties, including antioxidant, anti-cancer, anti-inflammatory, antimicrobial, anti-neurotoxic, and immunomodulatory activities.5–8 These beneficial effects can be attributed to the presence of bioactive compounds in mushrooms, such as ter  \npenoids, sterols, alkaloids, polyphenols, and fatty acids.9,10  \nSuillus granulatus (L.) Roussel, known as the “weeping bolete”, is an edible and medicinal mushroom that belongs to the family Boletaceae, order Boletales, class Agaricomycetesand phylum Basidiomycota.11–15 It is widely distributed around the world and has been used as a model organism for investigating symbiotic relationships with pine trees.16–18 Nevertheless, there is a limited amount of data available regarding the chemical composition and bioactivity of this  \naCollege of Life Sciences, Hebei Normal University, Shijiazhuang, Hebei 050024,  \nChina. E-mail: [lvjianhua@hebtu.edu.cn](lvjianhua@hebtu.edu.cn), [xiudou882003@163.com](xiudou882003@163.com)  \nbInstitute of Biology, Hebei Academy of Science, Shijiazhuang, Hebei 050081, China †Electronic supplementary information (ESI) available: Fig. S1–S47: HR-ESI-MSand NMR spectra of compounds 2–8; Fig. S48: The TIC of LC-MS/MS identification; Fig. S49: HSP90AA1 peptide mass spectrograms; Fig. S50: The original image of the full gel. See DOI: [https://doi.org/10.1039/d4fo01500b](https://doi.org/10.1039/d4fo01500b)  \nmushroom, having been reported only for its phenolic acids, polyprenylphenols, and antimicrobial and antitumor activities.19–22  \nLiver cancer is one of the most common malignant tumorsat present and the second leading lethal cancer worldwide.23,24 The current treatments for liver cancer include chemotherapy, radiation therapy, surgical resection, and targeted therapy.25 Although the treatments show a certain curative impact, they have some limitations such as large side effects, easy recurrence, and drug resistance.26,27 Therefore, there is an urgent need to find an effective and safe drug for the treatment of liver cancer. In this study, the chemical constituents of S. granulatus were characterized, and the anti-hepatocarcinoma activity and molecular targets of isolated compounds were explored.  \n2. Materials and methods  \n2.1. General experimental procedures  \n","cbCairtuBYK10YoB","https://ap.wps.com/l/cbCairtuBYK10YoB","pdf",1877325,1,11,"English","en",105,"# Introduction\n## Materials and methods\n### General experimental procedures\n### Mushroom material\n### Extraction and isolation","[{\"question\":\"How were the structures of the new polyprenylphenol compounds determined?\",\"answer\":\"Structures were elucidated using HR-ESI-MS and 1D/2D NMR data. Absolute configuration for compound 8 was assigned by comparing experimental and calculated ECD results.\"},{\"question\":\"Which compounds showed significant antitumor activity against HepG2 cells?\",\"answer\":\"Compounds 1 and 3–6 demonstrated significant antitumor activity compared with the positive control (cisplatin), with IC50 values ranging from 8.19 to 13.97 μM.\"},{\"question\":\"How was the direct molecular target of compound 5 identified?\",\"answer\":\"DARTS assay combined with LC-MS/MS analysis identified HSP90AA1 as the direct target of compound 5, and molecular docking verified the interaction between compound 5 and HSP90AA1.\"}]","Isolation and target identification of antihepatoma polyprenylphenols from the edible mushroom Suillus granulatus | PDF",1788210261,28,{"code":4,"msg":31,"data":32},"ok",{"site_id":24,"language":23,"slug":33,"title":13,"keywords":34,"description":14,"schema_data":35,"social_meta":87,"head_meta":89,"extra_data":91,"updated_unix":28},"isolation-and-target-identification-of-antihepatoma-polyprenylphenols-from-the-edible-mushroom-suillus-granulatus","",{"@graph":36,"@context":86},[37,54,69],{"@type":38,"itemListElement":39},"BreadcrumbList",[40,44,48,51],{"item":41,"name":42,"@type":43,"position":20},"https://docshare.wps.com","Home","ListItem",{"item":45,"name":46,"@type":43,"position":47},"https://docshare.wps.com/document/","Document",2,{"item":49,"name":12,"@type":43,"position":50},"https://docshare.wps.com/document/research-report/",3,{"item":52,"name":13,"@type":43,"position":53},"https://docshare.wps.com/document/isolation-and-target-identification-of-antihepatoma-polyprenylphenols-from-the-edible-mushroom-suillus-granulatus/167207/",4,{"url":52,"name":13,"@type":55,"author":56,"headline":13,"publisher":58,"fileFormat":61,"inLanguage":23,"description":14,"dateModified":62,"datePublished":63,"encodingFormat":61,"isAccessibleForFree":64,"interactionStatistic":65},"DigitalDocument",{"name":9,"@type":57},"Person",{"url":41,"name":59,"@type":60},"DocShare","Organization","application/pdf","2026-09-01","2026-08-31",true,{"@type":66,"interactionType":67,"userInteractionCount":20},"InteractionCounter",{"@type":68},"ViewAction",{"@type":70,"mainEntity":71},"FAQPage",[72,78,82],{"name":73,"@type":74,"acceptedAnswer":75},"How were the structures of the new polyprenylphenol compounds determined?","Question",{"text":76,"@type":77},"Structures were elucidated using HR-ESI-MS and 1D/2D NMR data. Absolute configuration for compound 8 was assigned by comparing experimental and calculated ECD results.","Answer",{"name":79,"@type":74,"acceptedAnswer":80},"Which compounds showed significant antitumor activity against HepG2 cells?",{"text":81,"@type":77},"Compounds 1 and 3–6 demonstrated significant antitumor activity compared with the positive control (cisplatin), with IC50 values ranging from 8.19 to 13.97 μM.",{"name":83,"@type":74,"acceptedAnswer":84},"How was the direct molecular target of compound 5 identified?",{"text":85,"@type":77},"DARTS assay combined with LC-MS/MS analysis identified HSP90AA1 as the direct target of compound 5, and molecular docking verified the interaction between compound 5 and HSP90AA1.","https://schema.org",{"og:url":52,"og:type":88,"og:title":13,"og:site_name":59,"og:description":14},"article",{"robots":90,"canonical":52},"index,follow",{"doc_id":7,"site_id":24},{"code":4,"msg":5,"data":93},[94,98,102,106,111,116,121,124,129,132,136],{"id":20,"doc_module":4,"doc_module_name":46,"category_name":95,"show_sort_weight":96,"slug":97},"Story & Novel",90,"story-novel",{"id":47,"doc_module":4,"doc_module_name":46,"category_name":99,"show_sort_weight":100,"slug":101},"Literature",80,"literature",{"id":53,"doc_module":4,"doc_module_name":46,"category_name":103,"show_sort_weight":104,"slug":105},"Exam",70,"exam",{"id":107,"doc_module":4,"doc_module_name":46,"category_name":108,"show_sort_weight":109,"slug":110},5,"Comic",60,"comic",{"id":112,"doc_module":4,"doc_module_name":46,"category_name":113,"show_sort_weight":114,"slug":115},6,"Technology",50,"technology",{"id":117,"doc_module":4,"doc_module_name":46,"category_name":118,"show_sort_weight":119,"slug":120},7,"Healthcare",40,"healthcare",{"id":11,"doc_module":4,"doc_module_name":46,"category_name":12,"show_sort_weight":122,"slug":123},30,"research-report",{"id":125,"doc_module":4,"doc_module_name":46,"category_name":126,"show_sort_weight":127,"slug":128},9,"Religion & Spirituality",20,"religion-spirituality",{"id":127,"doc_module":4,"doc_module_name":46,"category_name":130,"show_sort_weight":127,"slug":131},"World Cup","world-cup",{"id":133,"doc_module":4,"doc_module_name":46,"category_name":134,"show_sort_weight":133,"slug":135},10,"Lifestyle","lifestyle",{"id":137,"doc_module":4,"doc_module_name":46,"category_name":138,"show_sort_weight":107,"slug":139},19,"General","general"]