[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-398691-105":59,"doc-detail-398691-en":131},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":124,"head_meta":126,"extra_data":128,"updated_unix":130},105,"en","isolation-and-identification-of-staphylococcus-aureus-using-standard-methods","Isolation and Identification of Staphylococcus Aureus Using Standard Methods","","A methicillin resistant Staphylococcus aureus (MRSA) screening approach evaluates isolates through standardized bacteriological identification workflows rather than testing for MRSA alone. Thirty-six MRSA isolates were obtained and subjected to Gram staining and culture on selective media. Golden yellow colonies on mannitol salt agar and clustered Gram-positive cocci were observed, while mannitol fermenting and characteristic lactose fermentation patterns further supported presumptive identification. Tube coagulase performance can be enhanced by follow-up tests including mannitol salt agar, DNase, and tube coagulase. No single phenotypic test guarantees reliable identification of S. aureus.",{"@graph":69,"@context":123},[70,84,106],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/isolation-and-identification-of-staphylococcus-aureus-using-standard-methods/398691/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/isolation-and-identification-of-staphylococcus-aureus-using-standard-methods/398691.png","ImageObject",300,407,{"name":92,"@type":93},"Adam","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-11-02","2026-09-27",true,{"@type":102,"interactionType":103,"userInteractionCount":105},"InteractionCounter",{"@type":104},"ViewAction",16,{"@type":107,"mainEntity":108},"FAQPage",[109,115,119],{"name":110,"@type":111,"acceptedAnswer":112},"What was the sample source for isolating Staphylococcus aureus in this study?","Question",{"text":113,"@type":114},"Clinical specimens were pus samples including ear pus, pus from wounds, post-operative infections, boils, pimples, and furuncles.","Answer",{"name":116,"@type":111,"acceptedAnswer":117},"How were the isolates initially screened and presumptively identified?",{"text":118,"@type":114},"Suspected colonies were Gram stained and cultured on mannitol salt agar, with growth characteristics and Gram-positive clustered cocci used as key observations.",{"name":120,"@type":111,"acceptedAnswer":121},"Why did the study use multiple follow-up tests beyond tube coagulase?",{"text":122,"@type":114},"The study reports that there is no single phenotypic test, including tube coagulase, that can guarantee reliable identification of Staphylococcus aureus; efficiency can be improved by sequel testing such as mannitol salt agar and DNase.","https://schema.org",{"og:url":83,"og:type":125,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":127,"canonical":83},"index,follow",{"doc_id":129,"site_id":62},398691,1790734375,{"code":4,"msg":5,"data":132},{"doc_id":129,"user_id":133,"nickname":92,"user_avatar":134,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":135,"file_id":136,"file_url":137,"file_type":138,"file_size":139,"view_count":105,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":29,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145,"preview_page_count":4},1374404737137,"https://ap-avatar.wpscdn.com/davatar_155a257f0dc6eb9ab79c44ca47cae57d","RESEARCH ARTICLE  \nISOLATION AND IDENTIFICATION OF STAPHYLOCOCCUS AUREUS USING  \nSTANDARD METHODS  \nJolly Chauhan and Avadhesh Kumar Koshal*  \nMotherhood University, Roorkee, Haridwar, Uttarakhand PIN 247661  \nEmail: [akkoshal@hotmail.com](akkoshal@hotmail.com)  \nReceived-04.01.2021, Revised-15.01.2021, Accepted-29.01.2021  \nAbstract: A methicillin resistant S. aureus (MRSA) screen tests solely for the presence of MRSA and no other microbes. This test is used to screen for MRSA in patient in hospitals. The first strain of penicillin resistant S. aureus was isolated in London, England hospitals. Staphylococcus is a versatile organism with several virulent characteristics and resistance mechanisms. Multidrug resistance is now the norm among the Gram-positive bacteria pneumococci, enterococci and staphylococci. S. aureus is perhaps the pathogen of greatest concern because of its intrinsic virulence, its ability to cause a diverse array of life-threatening infections in humans and in various animal species, and its capacity to adapt to different environmental conditions. In the present study, a total of 36 isolates of MRSA were obtained. All the suspected colonies were gram stained and plated on manitol salt agar. Growth of golden yellow colonies of Staphylococcus aureus on manitol salt agar plate. On gram staining gram-positive cocci in clusters were observed on all the slides. On manitol salt agar, small yellow manitol fermenting colonies were obtained. On MacConkey agar small light pink lactose fermenting colonies were observed. In the present study, the efficiency of the tube coagulase test can be markedly improved by sequel testing of the isolates with Mannitol salt agar, DNase and Tube coagulase. There is no single phenotypic test (including tube coagulase) that can guarantee reliable results in the identification of Staphylococcus aureus.  \nKeywords: Gram-positive, MRSA, Resistant strain, Staphylococcus, Virulent  \nINTRODUCTION MATERIALS AND METHODS  \nStaphylococci are Gram-positive salt tolerant and spherical shaped bacteria. They are classified as  \ncoagulate positive (e.g Staph intermedius & Staphylococcus aureus) and coagulate negative (e.g S. epidermidis, S. heamolyticus, S. capitis, S. saprophyticus) (Funmilola A. Ayeni, B.T Odumosu. 2016) . The cocci are spherical, approximately 1µm in diameter, arranged in grape like clusters. They maybe found singly, in pairs or in short chains especially in liquid culture. They are non-motile and nonsporing and some strains possess microscopically visible capsules (Anathanarayan, 2002) . They grow readily on ordinary media within a temperature range of 10-42°C. Optimum temperature is 37°C and pH 7.4-7.6. On nutrient agar a typical 24hr S. aureus colonies are pigmented, smooth, entire, slightly raised, translucent and hemolytic on routine blood agar. Small colony variants (SCVs) of S. aureus produce colonies that are pinpoint in size, non hemolytic and non pigmented. In liquid medium, uniform turbidity is produced. Selective media used for isolating S. aureus contain 8-10% NaCl like saltmilk agar, ludlam’s medium containing lithium chloride and tellurite (Bannerman, 2003) . The Objectives of the present study are collection of clinical specimens of ear pus, pus from wounds and post operative infections, boils, pimples and furuncles. The Isolation and identification of Staphylococcus aureus isolates using standard techniques.  \nCollection and processing of clinical samples  \nThe clinical Microbiology specimen selected for this study were pus sample which included ear pus, pus from wounds and post operative infections, boils, pimples and furuncles.  \nThe specimens were collected at the hospital aseptically within 2 h of collection these were transported to the laboratory for further processing. Sheep blood agar and mannitol salt agar was used for inoculation.  \n(i) Nutrient Agar Media  \nFor the isolation of pure culture, Nutrient Agar Media (Hi-Media, Mumbai) was carefully weighed an","cbCaipL485ilEGwo","https://ap.wps.com/l/cbCaipL485ilEGwo","pdf",601571,"English","# Introduction\n# Materials and Methods\n## Collection and processing of clinical samples\n## Nutrient agar media\n## Mannitol salt agar media\n## Blood agar media\n## Coagulase product","[{\"question\":\"What was the sample source for isolating Staphylococcus aureus in this study?\",\"answer\":\"Clinical specimens were pus samples including ear pus, pus from wounds, post-operative infections, boils, pimples, and furuncles.\"},{\"question\":\"How were the isolates initially screened and presumptively identified?\",\"answer\":\"Suspected colonies were Gram stained and cultured on mannitol salt agar, with growth characteristics and Gram-positive clustered cocci used as key observations.\"},{\"question\":\"Why did the study use multiple follow-up tests beyond tube coagulase?\",\"answer\":\"The study reports that there is no single phenotypic test, including tube coagulase, that can guarantee reliable identification of Staphylococcus aureus; efficiency can be improved by sequel testing such as mannitol salt agar and DNase.\"}]","Isolation and Identification of Staphylococcus Aureus Using Standard Methods | PDF",1790488189,15]