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Isolated cells are characterized through multiple assays, including transcriptome sequencing to define LCSC gene expression and classify liver cancer subtypes tied to prognosis.",{"@graph":14,"@context":73},[15,34,56],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/research-report/","Research & Report",3,{"item":32,"name":10,"@type":21,"position":33},"https://docshare.wps.com/document/isolation-and-identification-of-patient-derived-liver-cancer-stem-cells-and-development-of-personalized-treatment-strategies/329444/",4,{"url":32,"name":10,"@type":35,"image":36,"author":41,"headline":10,"publisher":44,"fileFormat":47,"inLanguage":8,"description":12,"dateModified":48,"datePublished":49,"encodingFormat":47,"isAccessibleForFree":50,"interactionStatistic":51},"DigitalDocument",{"url":37,"@type":38,"width":39,"height":40},"https://docshare.wps.com/thumbnails/isolation-and-identification-of-patient-derived-liver-cancer-stem-cells-and-development-of-personalized-treatment-strategies/329444.png","ImageObject",300,407,{"name":42,"@type":43},"Quinn","Person",{"url":19,"name":45,"@type":46},"DocShare","Organization","application/pdf","2026-11-03","2026-09-21",true,{"@type":52,"interactionType":53,"userInteractionCount":55},"InteractionCounter",{"@type":54},"ViewAction",7,{"@type":57,"mainEntity":58},"FAQPage",[59,65,69],{"name":60,"@type":61,"acceptedAnswer":62},"What challenge does the study address regarding liver cancer stem cells?","Question",{"text":63,"@type":64},"Heterogeneity in molecular markers of LCSCs has hindered development of effective isolation methods.","Answer",{"name":66,"@type":61,"acceptedAnswer":67},"How were LCSCs isolated and cultured in this study?",{"text":68,"@type":64},"The study introduced an approach to isolate and culture LCSCs from human primary liver cancer using MEFs as feeder cells with a defined medium.",{"name":70,"@type":61,"acceptedAnswer":71},"What key findings support the existence and stability of the isolated LCSCs?",{"text":72,"@type":64},"Multiple cell strains were maintained long-term with colony growth on MEFs, cloned cells formed tumors in immunodeficient mice, and cryopreserved cells retained colony-growth features after recovery.","https://schema.org",{"og:url":32,"og:type":75,"og:title":10,"og:site_name":45,"og:description":12},"article",{"robots":77,"canonical":32},"index,follow",{"doc_id":79,"site_id":7},329444,1790006897,{"code":4,"msg":82,"data":83},"success",[84,88,92,96,101,106,110,114,119,122,126],{"id":22,"doc_module":4,"doc_module_name":25,"category_name":85,"show_sort_weight":86,"slug":87},"Story & Novel",90,"story-novel",{"id":26,"doc_module":4,"doc_module_name":25,"category_name":89,"show_sort_weight":90,"slug":91},"Literature",80,"literature",{"id":33,"doc_module":4,"doc_module_name":25,"category_name":93,"show_sort_weight":94,"slug":95},"Exam",70,"exam",{"id":97,"doc_module":4,"doc_module_name":25,"category_name":98,"show_sort_weight":99,"slug":100},5,"Comic",60,"comic",{"id":102,"doc_module":4,"doc_module_name":25,"category_name":103,"show_sort_weight":104,"slug":105},6,"Technology",50,"technology",{"id":55,"doc_module":4,"doc_module_name":25,"category_name":107,"show_sort_weight":108,"slug":109},"Healthcare",40,"healthcare",{"id":111,"doc_module":4,"doc_module_name":25,"category_name":29,"show_sort_weight":112,"slug":113},8,30,"research-report",{"id":115,"doc_module":4,"doc_module_name":25,"category_name":116,"show_sort_weight":117,"slug":118},9,"Religion & Spirituality",20,"religion-spirituality",{"id":117,"doc_module":4,"doc_module_name":25,"category_name":120,"show_sort_weight":117,"slug":121},"World Cup","world-cup",{"id":123,"doc_module":4,"doc_module_name":25,"category_name":124,"show_sort_weight":123,"slug":125},10,"Lifestyle","lifestyle",{"id":127,"doc_module":4,"doc_module_name":25,"category_name":128,"show_sort_weight":97,"slug":129},19,"General","general",{"code":4,"msg":82,"data":131},{"doc_id":79,"user_id":132,"nickname":42,"user_avatar":133,"doc_module":4,"category_id":111,"category_name":29,"doc_title":10,"doc_description":12,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":55,"is_deleted":4,"is_public":22,"is_downloadable":22,"audit_status":22,"page_count":139,"language":140,"language_code":8,"site_id":7,"html_lang":8,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":12,"update_tm":144,"read_time":145,"preview_page_count":4},962075114765,"https://ap-avatar.wpscdn.com/davatar_a8503ba1806abce46bf441b54a3ca4cd","Guo et al. Journal of Translational Medicine (2024) 22:1036  \n[https://doi.org/10.1186/s12967-024-05870-9](https://doi.org/10.1186/s12967-024-05870-9)  \nJournal of Translational Medicine  \nRESEARCH Open Access  \nIsolation and identification of patient-derived  liver cancer stem cells and development of personalized treatment strategies  \nTingting Guo 1,2†, Shuai Zhang3†, Weiping Zeng2, Yan Liang2, Jinghe Xie4, ShouPei Liu 1,2, Yaqi Qiu2, Yingjie Fu 1,2, Yimeng Ou5, Keqiang Ma6, Bailin Wang7, Weil i Gu3,11* and Yuyou Duan 1,2,8,9,10*  \nAbstract  \nBackground Liver cancer stem cells (LCSCs) are thought to drive the metastasis and recurrence, however, the heterogeneity of molecular markers of LCSCs has hindered the development of effective methods to isolate them. Methods This study introduced an effective approach to isolate and culture LCSCs from human primary liver cancer (HPLC), leveraging mouse embryonic fibroblasts (MEFs) as feeder cells in conjunction with using defined medium. Isolated LCSCs were further characterized by multiple approaches. Transcriptome sequencing data analysis was conducted to identify highly expressed genes in LCSCs and classify different subtypes of liver cancers.  \nResults Total sixteen cell strains were directly isolated from 24 tissues of three types of HPLC without sorting, seven of which could be maintained long-term culture as colony growth on MEFs, which is unique characteristics of stem cells. Even 10 of cloned cells formed the tumors in immunodeficient mice, indicating that those cloned cells were tumorgenic. The histologies and gene expression pattern of human xenografts were very similar to those of HPLC where these cloned cells were isolated. Moreover, putative markers of LCSCs were further verified to all express in cloned cells, confirming that these cells were LCSCs. These cloned LCSCs could be cryopreserved, and still maintained the feature of colony growth on MEFs after the recovery. Compared to suspension culture as conventional approach to culture LCSCs, our approach much better maintained stemness of LCSCs for a long time. To date, these cloned cells could be cultured on MEFs over 12 passages. Moreover, bioinformatics analysis of sequencing data revealed the gene expression profiles in LCSCs, and liver cancers were classified into two subtypes C1 and C2 based on genes associated with the prognosis of LCSCs. Patients of the C2 subtype, which is closely related to the extracellular matrix, were found to be sensitive to treatments such as Cisplatin, Axitinib, JAK1 inhibitors, WNT-c59, Sorafenib, and RO-3306 .  \nConclusion In summary, this effective approach offers new insights into the molecular landscape of human liver cancers, and the identification of the C2 subtype and its unique response to the treatment pave the way for the creation of more effective, personalized therapeutic strategies.  \n†Tingting Guo and Shuai Zhang contributed equally to this work.  \n*Correspondence:  \nWeil i Gu [lili-6423@163.com](lili-6423@163.com)[ ](lili-6423@163.com)Yuyou Duan [yuyouduan@scut.edu.cn](yuyouduan@scut.edu.cn)  \nFull list of author information is available at the end of the article  \n© The Author(s) 2024. Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not p","cbCaij3gV4nz4IwY","https://ap.wps.com/l/cbCaij3gV4nz4IwY","pdf",4918946,21,"English","# Abstract\n## Background\n## Methods\n## Results\n## Conclusion\n# Introduction","[{\"question\":\"What challenge does the study address regarding liver cancer stem cells?\",\"answer\":\"Heterogeneity in molecular markers of LCSCs has hindered development of effective isolation methods.\"},{\"question\":\"How were LCSCs isolated and cultured in this study?\",\"answer\":\"The study introduced an approach to isolate and culture LCSCs from human primary liver cancer using MEFs as feeder cells with a defined medium.\"},{\"question\":\"What key findings support the existence and stability of the isolated LCSCs?\",\"answer\":\"Multiple cell strains were maintained long-term with colony growth on MEFs, cloned cells formed tumors in immunodeficient mice, and cryopreserved cells retained colony-growth features after recovery.\"}]","Isolation and identification of patient-derived liver cancer stem cells and development of personalized treatment strategies | PDF",1789984151,53]