[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-640113-105":59,"doc-detail-640113-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","invision-his-tag-in-gel-stain-experienced-users-guide","InVision - His-tag In-gel Stain Experienced Users Guide","","Quick reference guide for experienced users of InVision™ His-tag In-gel Stain, describing protocol steps from electrophoresis through fixation, washing, staining, and immediate visualization. Provides key incubation conditions (room temperature, orbital shaking) and timing, plus imaging requirements using UV transilluminators or laser-based scanners with specified excitation/emission wavelengths (560/590 nm). Includes product description, storage guidance for stain and His-tagged protein standard, expected results, and troubleshooting. For research use only.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/invision-his-tag-in-gel-stain-experienced-users-guide/640113/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/invision-his-tag-in-gel-stain-experienced-users-guide/640113.png","ImageObject",300,407,{"name":92,"@type":93},"I feel Different","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-11-09","2026-10-22",true,{"@type":102,"interactionType":103,"userInteractionCount":24},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"How should incubation and shaking be performed during staining?","Question",{"text":112,"@type":113},"Perform all incubations at room temperature and on an orbital shaker set at 1 revolution per second.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"What imaging wavelengths are specified for InVision™ His-tag In-gel Stain?",{"text":117,"@type":113},"The maximum excitation wavelength is 560 nm and the maximum emission wavelength is 590 nm.",{"name":119,"@type":110,"acceptedAnswer":120},"How long does it take to complete staining of a mini-gel?",{"text":121,"@type":113},"Staining of a mini-gel is complete in less than 3 hours.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},640113,1793118288,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":24,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145,"preview_page_count":4},962093541761,"https://ap-avatar.wpscdn.com/davatar_29158cc5080c5b710cf443261637dec0","USER GUIDE  \nInVision ™ His-tag In-gel Stain  \nFor specific, sensitive staining of His-tagged fusion proteins  \nCatalog numbers LC6030, LC6033  \nRevision Date 19 March 2012  \nPublication part number 25-0671  \nMAN0000397  \nFor Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.  \nii  \nContents  \nInVision™ His-tag In-gel Stain Experienced Users Guide .................... v  \n[Contents and Storage ...............................................................................vi](Contents and Storage ...............................................................................vi)  \nIntroduction .........................................................................1  \nProduct Description................................................................................... 1  \nExperimental Overview ............................................................................ 3  \nMethods ...............................................................................4  \nUsing InVision™ His-tag In-gel Stain ...................................................... 4  \nVisualizing and Imaging the Gel ........................................................... 11  \nDownstream Applications...................................................................... 13  \nExpected Results ...................................................................................... 15  \nTroubleshooting ....................................................................................... 16  \nAppendix............................................................................18  \nAccessory Products.................................................................................. 18  \nTechnical Support .................................................................................... 19  \nPurchaser Notification ............................................................................ 21  \niv  \nInVision His-tag In-gel Stain Experienced  \n™  \nUsers Guide  \nIntroduction This quick reference sheet is included for experienced users of InVision™ His-tag In-gel Stain. If you are a first time user, follow the detailed protocol included in this manual.  \n\n| Step | Action |\n| --- | --- |\n| Perform\u003Cbr>Electrophoresis | Perform electrophoresis of His-tagged fusion proteins on a suitable gel. Proceed to staining. |\n| Staining Procedure | Perform all incubations at room temperature and on an orbital shaker set at 1 revolution per second. See page 4 for specific protocol based on the gel type.\u003Cbr>1. After electrophoresis, fix the gel for 1 hour in the appropriate Fixing Solution (page 6) based on the gel type. Decant the fixative.\u003Cbr>2. Wash the gel twice for 10 minutes each with ultrapure water to remove the fixative.\u003Cbr>3. Stain the gel with the ready-to-use solution of InVision™ His-tag In-gel Stain for 1 hour at room temperature. Decant the stain.\u003Cbr>4. Wash the gel twice for 10 minutes each with 20 mM phosphate buffer, pH 7.8. Proceed to visualizing the gel immediately. |\n| Visualize the Gel | Place the gel on a UV transilluminator equipped with a camera (capable of integration) or use a laser-based scanner to capture an image of the gel. The maximum excitation wavelength for InVision™ His-tag In-gel Stain is at 560 nm and maximum emission wavelength is at 590 nm.\u003Cbr>InVision™ His-tag In-gel Staining will result in fluorescent His-tagged fusion protein bands. |\n\nContents and Storage  \nProducts This manual is supplied with the following products:  \n\n| Product | Quantity | Catalog no. |\n| --- | --- | --- |\n| InVision™ His-tag In-gel Stain | 500 mL | LC6030 |\n| InVision™ His-tag In-gel Staining Kit | 500 mL InVision™ His-tag In-gel Stain\u003Cbr>125 μL BenchMark™\u003Cbr>His-tagged Protein Standard | LC6033 |\n\nContents InVision™ His-tag In-gel Stain is shipped at room  \nand Storage temperature in an amber bottle. Sufficient stain is supplied for staining 20 mini-gels.  \nUpon receipt, store InVision™ His-tag In-gel Stain at room temperature,","cbCaik3z28xIai0h","https://ap.wps.com/l/cbCaik3z28xIai0h","pdf",446252,32,"English","# Introduction\n## Product Description\n## Experimental Overview\n## Methods\n# Using InVision™ His-tag In-gel Stain\n## Performing Electrophoresis\n## Staining Procedure\n## Visualizing and Imaging the Gel\n# Downstream Applications\n# Expected Results\n# Troubleshooting\n# Appendix\n## Accessory Products\n## Technical Support\n## Purchaser Notification","[{\"question\":\"How should incubation and shaking be performed during staining?\",\"answer\":\"Perform all incubations at room temperature and on an orbital shaker set at 1 revolution per second.\"},{\"question\":\"What imaging wavelengths are specified for InVision™ His-tag In-gel Stain?\",\"answer\":\"The maximum excitation wavelength is 560 nm and the maximum emission wavelength is 590 nm.\"},{\"question\":\"How long does it take to complete staining of a mini-gel?\",\"answer\":\"Staining of a mini-gel is complete in less than 3 hours.\"}]","InVision - His-tag In-gel Stain Experienced Users Guide | PDF",1792707908,81]