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The work evaluated Moleaer oxygen and ozone nanobubbles as an innovative chemical-free approach and examined the fate of cyanobacteria, cyanotoxins, and non-target eukaryotic algae following exposure. The report outlines required final-report sections, including project background and timeline, financial performance versus budget, schedule versus completion, activities completed, monitoring results and data interpretation, benefits realization, and supporting photo documentation, figures, tables, and attachments.",{"@graph":14,"@context":72},[15,34,55],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/research-report/","Research & 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nanobubble technology was evaluated in the INV017 project?","Question",{"text":62,"@type":63},"The project evaluated Moleaer oxygen and ozone nanobubbles as an innovative technology for mitigating and controlling harmful algal blooms.","Answer",{"name":65,"@type":60,"acceptedAnswer":66},"Where was the grant work conducted?",{"text":67,"@type":63},"The work took place at Pahokee Marina and Campground on Lake Okeechobee, in Pahokee, Florida, under the South Florida Water Management District.",{"name":69,"@type":60,"acceptedAnswer":70},"How did the project assess effectiveness and monitoring outcomes?",{"text":71,"@type":63},"Effectiveness was assessed through spatially patterned sampling, an in-situ monitoring setup with multiple sensors at treatment and control locations, and discrete samples analyzed by a NELAC-certified 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Florida Gulf Coast University |  |  |\n| Grantee Address: | 10501 FGCU Blvd. South, Fort Myers, FL 33965-6565 |  |  |\n| Grantee’s Grant Manager: | Donna Gilmore | Telephone No.: | (239) 590-7022 |\n| Reporting Period: | July 1, 2020 – October 15, 2023 |  |  |\n| Project Number and Title: | INV017 Harmful Algal Bloom Innovative Technology Project: Chemical-Free Harmful Algal Bloom Control |  |  |\n| This Task 5, Final Report includes:\u003Cbr>􀂃 Section 1: Project location and background, project description and timeline, grant award amount and anticipated benefits.\u003Cbr>􀂃 Section 2: Financial summary of actual costs versus the budget, along with any changes required to the budget. Include any match or locally pledged contributions provided, along with other related project work performed outside of this Agreement to identify the overall project cost.\u003Cbr>􀂃 Section 3: Discussion of project schedule versus actual completion, including changes required to the schedule, unexpected site conditions and adjustments, significant unexpected delays, and corrections, and/or other significant deviations from the original project plan.\u003Cbr>􀂃 Section 4: Summary of activities completed as well as those not completed and why, as well as a brief summary of any additional phases yet to be completed.\u003Cbr>􀂃 Section 5: Photo documentation of work performed (before, during and after), appropriate figures (site location, site plan[s]. etc.), appropriate tables summarizing data/information relevant to Grant Work Plan tasks, and appropriate attachments relevant to the project.\u003Cbr>􀂃 Section 6: Discussion of whether the anticipated benefits have been/will be realized (e.g., why a Best Management Practice (BMP) approach did or did not exceed the expected removal efficiency) .\u003Cbr>􀂃 Section 7: Summary of monitoring activities completed and any not completed and why, monitoring results, and an interpretation of data based on planned versus realized results.\u003Cbr>Section 1: Project location and background, project description and timeline, grant award amount and anticipated benefits.\u003Cbr>This project used nanobubble injection technology to address persistent and hard to treat freshwater harmful algae blooms (HABs) in Pahokee, Florida. The goal of this project was to comprehensively evaluate the use of Moleaer oxygen and ozone nanobubbles as an innovative technology for the mitigation and control of HABs and to evaluate the fate of cyanobacteria, their cyanotoxins, and the non-target eukaryotic algae after nanobubble exposure in the Lake Okeechobee Pahokee Marina.\u003Cbr>Nanobubbles are high-pressure nanosized bubbles that have several unique properties that distinguish them from larger bubbles. They are extremely small with an approximate diameter of 100nm which enables the generation of high concentrations of 10^7 or higher bubbles per mL. The high surface area from the bubble concentration contributes to hyper-efficient oxygenation. Further, they create a mild oxidative stress that has been suggested to impact the viability of cyanobacterial cells. Intrinsic to the property of the bubble itself and not the gas that it contains, nanobubbles have shown to generate reactive oxygen species upon bubble collapse resulting in an oxidation effect that degrades easily oxidizable organic materials, which may lead to the degradation of cyanobacteria and their toxins. |  |  |  |\n\nThe INV17 grant project took place within the South Florida Water Management District at Pahokee Marina and Campground [190 N Lake Ave, Pahokee, FL 33476 (26.825080, -80.667972)] located on the southeast corner of Lake Okeechobee as shown in Figure 1. The marina was chosen as an optimal location to conduct the grant work given its size, history of intense and routine blue-green algal blooms, and need for a novel remediation strategy to address the persistent algae blooms.  \nFigure 1: The location of Pah","cbCaigBNeTWzcLzN","https://ap.wps.com/l/cbCaigBNeTWzcLzN","pdf",13922210,74,"English","# Section 1: Project location and background, project description and timeline, grant award amount and anticipated benefits\n# Section 2: Financial summary and budget changes\n# Section 3: Schedule discussion versus actual completion\n# Section 4: Activities completed and not completed\n# Section 5: Photo documentation, figures, tables, and attachments\n# Section 6: Anticipated benefits realization (BMP performance)\n# Section 7: Monitoring activities, results, and data interpretation","[{\"question\":\"What nanobubble technology was evaluated in the INV017 project?\",\"answer\":\"The project evaluated Moleaer oxygen and ozone nanobubbles as an innovative technology for mitigating and controlling harmful algal blooms.\"},{\"question\":\"Where was the grant work conducted?\",\"answer\":\"The work took place at Pahokee Marina and Campground on Lake Okeechobee, in Pahokee, Florida, under the South Florida Water Management District.\"},{\"question\":\"How did the project assess effectiveness and monitoring outcomes?\",\"answer\":\"Effectiveness was assessed through spatially patterned sampling, an in-situ monitoring setup with multiple sensors at treatment and control locations, and discrete samples analyzed by a NELAC-certified laboratory.\"}]","INV017 Harmful Algal Bloom Innovative Technology Project - Chemical-Free Harmful Algal Bloom Control - Deliverable 5 Final Report | PDF",186]