[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"doc-seo-330155-105":3,"detail-sidebar-cat-0-en-105":81,"doc-detail-330155-en":130},{"code":4,"msg":5,"data":6},0,"ok",{"site_id":7,"language":8,"slug":9,"title":10,"keywords":11,"description":12,"schema_data":13,"social_meta":74,"head_meta":76,"extra_data":78,"updated_unix":80},105,"en","intraoperative-flow-cytometry-for-the-rapid-diagnosis-and-validation-of-surgical-clearance-of-non-melanoma-skin-cancer-a-prospective-clinical-feasibility-study","Intraoperative Flow Cytometry for the Rapid Diagnosis and Validation of Surgical Clearance of Non-Melanoma Skin Cancer - A Prospective Clinical Feasibility Study","","Non-melanoma skin cancer (NMSC) is the most prevalent cancer in humans, with a high global incidence. A prospective clinical feasibility study evaluates intraoperative flow cytometry (iFC) for instant diagnosis and complete surgical clearance of NMSC. Thirty recruited patients undergoing NMSC assessment were analyzed. iFC showed high sensitivity (95.2%) and specificity (87.1%), with accuracy 91.1% by ROC analysis. Tumors were mainly diploid, with some hypoploid cases, and iFC supported precise intraoperative characterization and margin evaluation.",{"@graph":14,"@context":73},[15,34,56],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/healthcare/","Healthcare",3,{"item":32,"name":10,"@type":21,"position":33},"https://docshare.wps.com/document/intraoperative-flow-cytometry-for-the-rapid-diagnosis-and-validation-of-surgical-clearance-of-non-melanoma-skin-cancer-a-prospective-clinical-feasibility-study/330155/",4,{"url":32,"name":10,"@type":35,"image":36,"author":41,"headline":10,"publisher":44,"fileFormat":47,"inLanguage":8,"description":12,"dateModified":48,"datePublished":49,"encodingFormat":47,"isAccessibleForFree":50,"interactionStatistic":51},"DigitalDocument",{"url":37,"@type":38,"width":39,"height":40},"https://docshare.wps.com/thumbnails/intraoperative-flow-cytometry-for-the-rapid-diagnosis-and-validation-of-surgical-clearance-of-non-melanoma-skin-cancer-a-prospective-clinical-feasibility-study/330155.png","ImageObject",300,407,{"name":42,"@type":43},"Patrick","Person",{"url":19,"name":45,"@type":46},"DocShare","Organization","application/pdf","2026-10-23","2026-09-21",true,{"@type":52,"interactionType":53,"userInteractionCount":55},"InteractionCounter",{"@type":54},"ViewAction",10,{"@type":57,"mainEntity":58},"FAQPage",[59,65,69],{"name":60,"@type":61,"acceptedAnswer":62},"What clinical purpose does intraoperative flow cytometry (iFC) serve in this study?","Question",{"text":63,"@type":64},"iFC is used for the instant diagnosis of non-melanoma skin cancer and for validating complete surgical clearance by evaluating surgical margins during operations.","Answer",{"name":66,"@type":61,"acceptedAnswer":67},"How many patients were included, and what diagnostic performance was reported?",{"text":68,"@type":64},"Thirty patients were recruited. iFC achieved sensitivity of 95.2%, specificity of 87.1%, and overall accuracy of 91.1% based on ROC analysis.",{"name":70,"@type":61,"acceptedAnswer":71},"What did the results show about tumor DNA ploidy and related metrics?",{"text":72,"@type":64},"Most tumors were diploid, while two cases were hypoploid. The study also reports differences in G0/G1 fractions between normal and tumor tissues and an increase in tumor index in cancer cells.","https://schema.org",{"og:url":32,"og:type":75,"og:title":10,"og:site_name":45,"og:description":12},"article",{"robots":77,"canonical":32},"index,follow",{"doc_id":79,"site_id":7},330155,1790151169,{"code":4,"msg":82,"data":83},"success",[84,88,92,96,101,106,110,115,120,123,126],{"id":22,"doc_module":4,"doc_module_name":25,"category_name":85,"show_sort_weight":86,"slug":87},"Story & Novel",90,"story-novel",{"id":26,"doc_module":4,"doc_module_name":25,"category_name":89,"show_sort_weight":90,"slug":91},"Literature",80,"literature",{"id":33,"doc_module":4,"doc_module_name":25,"category_name":93,"show_sort_weight":94,"slug":95},"Exam",70,"exam",{"id":97,"doc_module":4,"doc_module_name":25,"category_name":98,"show_sort_weight":99,"slug":100},5,"Comic",60,"comic",{"id":102,"doc_module":4,"doc_module_name":25,"category_name":103,"show_sort_weight":104,"slug":105},6,"Technology",50,"technology",{"id":107,"doc_module":4,"doc_module_name":25,"category_name":29,"show_sort_weight":108,"slug":109},7,40,"healthcare",{"id":111,"doc_module":4,"doc_module_name":25,"category_name":112,"show_sort_weight":113,"slug":114},8,"Research & Report",30,"research-report",{"id":116,"doc_module":4,"doc_module_name":25,"category_name":117,"show_sort_weight":118,"slug":119},9,"Religion & Spirituality",20,"religion-spirituality",{"id":118,"doc_module":4,"doc_module_name":25,"category_name":121,"show_sort_weight":118,"slug":122},"World Cup","world-cup",{"id":55,"doc_module":4,"doc_module_name":25,"category_name":124,"show_sort_weight":55,"slug":125},"Lifestyle","lifestyle",{"id":127,"doc_module":4,"doc_module_name":25,"category_name":128,"show_sort_weight":97,"slug":129},19,"General","general",{"code":4,"msg":82,"data":131},{"doc_id":79,"user_id":132,"nickname":42,"user_avatar":133,"doc_module":4,"category_id":107,"category_name":29,"doc_title":10,"doc_description":12,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":55,"is_deleted":4,"is_public":22,"is_downloadable":22,"audit_status":22,"page_count":139,"language":140,"language_code":8,"site_id":7,"html_lang":8,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":12,"update_tm":144,"read_time":113},549758146520,"https://ap-avatar.wpscdn.com/avatar/80002397d8c0411e94?_k=1775819394049821470","cancers   \nArticle  \nIntraoperative Flow Cytometry for the Rapid Diagnosis and Validation of Surgical Clearance of Non-Melanoma Skin Cancer:  \nA Prospective Clinical Feasibility Study  \nGeorgios Markopoulos 1, Evangeli Lampri 2, Ioulia Tragani 2, Nikolaos Kourkoumelis 3, Georgios Vartholomatos 1 and Konstantinos Seretis 4, *  \nCitation: Markopoulos, G.; Lampri, E.; Tragani, I.; Kourkoumelis, N.;  \nVartholomatos, G.; Seretis, K. Intraoperative Flow Cytometry for the Rapid Diagnosis and Validation of Surgical Clearance of Non-Melanoma Skin Cancer: A Prospective Clinical Feasibility Study. Cancers 2024, 16, 682 . [https://doi.org/10.3390/](https://doi.org/10.3390/)[ ](https://doi.org/10.3390/)cancers16040682  \nAcademic Editor: Ángele Juarranz  \nReceived: 4 January 2024  \nRevised: 20 January 2024  \nAccepted: 2 February 2024  \nPublished: 6 February 2024  \nCopyright: © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ([https://](https://)[ ](https://)[creativecommons.org/licenses/by/](creativecommons.org/licenses/by/)[ ](creativecommons.org/licenses/by/)[4.0/](4.0/)) .  \n1 Haematology Laboratory-Unit of Molecular Biology and Translational Flow Cytometry, University Hospital of Ioannina, 45110 Ioannina, Greece; [geomarkop@gmail.com](geomarkop@gmail.com) (G.M.); [gvarthol@gmail.com](gvarthol@gmail.com) (G.V.)  \n2 Department of Pathology, Medical School, University of Ioannina, 45110 Ioannina, Greece;  \n[elampri@uoi.gr](elampri@uoi.gr) (E.L.); [itragani@hotmail.com](itragani@hotmail.com) (I.T.)  \n3 Department of Medical Physics, Medical School, University of Ioannina, 45110 Ioannina, Greece; [nkourkou@uoi.gr](nkourkou@uoi.gr)  \n4 Department of Plastic Surgery, Medical School, University of Ioannina, 45110 Ioannina, Greece  \n* Correspondence: [drseretis@uoi.gr](drseretis@uoi.gr); Tel.: +30-2651099077  \nSimple Summary: There is escalating incidence of non-melanoma skin cancer (NMSC), which is the most prevalent cancer in humans. Our current research introduces intraoperative flow cytometry (iFC) as a diagnostic tool for NMSC and its surgical margins. Flow cytometry is instrumental in measuring and quantifying cell attributes. We conducted a pilot study involving patients diagnosed with NMSC. Our findings underscore the potential of iFC as an invaluable tool in characterizing NMSC and ensuring tumor-free resection margins. Given the technique’s proven efficacy in other cancer types, we believe iFC offers a promising avenue for enhancing complete tumor excision rates in NMSC treatment.  \nAbstract: Non-melanoma skin cancer (NMSC) is the most prevalent cancer in humans, with a high global incidence. We present a prospective clinical feasibility study on the use of intraoperative flow cytometry (iFC) for the instant diagnosis of NMSC and its complete surgical clearance. Flow cytometry, a laser-based technique, quantifies cell features, which has applications in cancer research. This study aim is to explore the potential applicability of iFC in detecting and characterizing NMSC and its surgical margins. In total, 30 patients who underwent diagnosis for NMSC were recruited. The method demonstrated high sensitivity (95.2%) and specificity (87.1%), with an accuracy of 91.1%, as confirmed with a receiver operating characteristic curve analysis. The results also indicated that most tumors were diploid, with two cases being hypoploid. The average G0/G1 fractions for normal and tumor tissue samples were 96.03 ± 0.30% and 88.03 ± 1.29%, respectively, with the tumor index escalating from 3.89 ± 0.30% to 11.95 ± 1.29% in cancerous cells. These findings underscore iFC’s capability for precise intraoperative NMSC characterization and margin evaluation, promising enhanced complete tumor excision rates. Given the technique’s successful application in various other malignancies, its implementation in NMSC diagnosis and ","cbCaitgXI71332Mu","https://ap.wps.com/l/cbCaitgXI71332Mu","pdf",1726962,12,"English","# Introduction\n## Background and incidence\n## Risk factors\n## Burden and clinical impact\n## Current treatment and surgical margin challenges","[{\"question\":\"What clinical purpose does intraoperative flow cytometry (iFC) serve in this study?\",\"answer\":\"iFC is used for the instant diagnosis of non-melanoma skin cancer and for validating complete surgical clearance by evaluating surgical margins during operations.\"},{\"question\":\"How many patients were included, and what diagnostic performance was reported?\",\"answer\":\"Thirty patients were recruited. iFC achieved sensitivity of 95.2%, specificity of 87.1%, and overall accuracy of 91.1% based on ROC analysis.\"},{\"question\":\"What did the results show about tumor DNA ploidy and related metrics?\",\"answer\":\"Most tumors were diploid, while two cases were hypoploid. The study also reports differences in G0/G1 fractions between normal and tumor tissues and an increase in tumor index in cancer cells.\"}]","Intraoperative Flow Cytometry for the Rapid Diagnosis and Validation of Surgical Clearance of Non-Melanoma Skin Cancer - A Prospective Clinical Feasibility Study | PDF",1789987439]